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Molecular Markers and Their Application in Breeding of Holstein Cattle for Resistance to Heat Stress

A technology of Holstein cattle and heat stress resistance, applied in the field of molecular genetics, can solve the problems of the application of heat shock protein 70, etc., achieve the effects of convenient and fast detection, early auxiliary selection, and low detection cost

Inactive Publication Date: 2011-12-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of heat shock protein 70 in the identification of heat stress resistance genes in Holstein cattle

Method used

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  • Molecular Markers and Their Application in Breeding of Holstein Cattle for Resistance to Heat Stress
  • Molecular Markers and Their Application in Breeding of Holstein Cattle for Resistance to Heat Stress
  • Molecular Markers and Their Application in Breeding of Holstein Cattle for Resistance to Heat Stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1 DNA extraction

[0030] Take the blood sample, tissue sample or milk sample (choose one of the three samples) of the dairy cow to be tested, according to Jin Dongyan, etc. [1] Wada rain [2] method to extract DNA from the sample.

[0031] 2 Apply the primers SEQ ID No.1 and SEQ ID No.2 screened by the present invention to carry out PCR-SSCP analysis on the extracted DNA and standard DNA, and carry out non-denaturing polyacrylamide electrophoresis to the amplified product, and analyze the electrophoretic pattern To carry out gene polymorphism analysis, the specific operation process is as follows:

[0032] Use the above primers to carry out PCR amplification on the PE 9600 amplification instrument: the PCR reaction volume is 12.5 μl, of which 10×buffer 1.25 μl, 25mM MgCl 2 0.8μl, 2.5mM dNTPs 1μl, Taq enzyme (5u / μl) 0.25μl, template DNA 100ng, 20μM primers 0.6μl each, add water to 12.5μl. The PCR reaction system was DNA pre-denatured at 94°C for 5 min, denatured at ...

Embodiment 2

[0038] Example 2: Type AA individuals have stable heredity.

[0039] According to follow-up studies, it has been proved that the parents of AA-type cows have both parents of the A gene (both are AA homozygous; or one is homozygous AA, and the other is AB or AC heterozygous; or both are AA homozygous; Both parties are AB or AC heterozygous), which proves that the AA gene can be inherited figure 2 .

Embodiment 3

[0040] Example 3: Relationship between different genotypes in the regulatory region of Holstein cattle HSP70 gene and heat stress resistance

[0041] 1. Gene frequency and genotype frequency of Holstein cattle heat stress resistance (HSP70 gene regulatory region) locus

[0042] According to the determination method described above in the content of the invention, Holstein cattle blood samples were collected, and the polymorphism of the genetic marker was detected. The results are shown in Table 1.

[0043] Table 1 Genotypes of Holstein Cattle

[0044]

[0045] 2. Changes in rectal temperature, respiratory rate and milk production of cows with different genotypes

[0046] Using SPSS11.5 software, the rectal temperature, respiratory rate and milk production of different genotypes of Holstein cattle were analyzed. The results are shown in Table 2 and Table 3.

[0047] Table 2 Rectal temperature and respiratory rate of each genotype of Holstein cattle

[0048]

[0049]

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Abstract

The invention belongs to the field of molecular genetics, and relates to molecular marker of heat stress resistant breeding of a Hostein cattle and application thereof. The molecular marker is SEQ ID NO.3. The molecular marker can be used to assistantly breed the Hostein cattle with the characteristic of heat stress resistance and higher milk yield.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to molecular markers and applications of Holstein cattle heat stress resistance breeding. Background technique [0002] Dairy cows can produce a series of physiological reactions in a high-temperature (high-humidity) environment, and the phenomenon of heat stress occurs, manifested as decreased appetite and reduced feed intake; increased body temperature, faster breathing; decreased absorption and utilization of nutrients; Endocrine dysfunction leads to a significant decrease in milk production, prolongation of the estrus cycle, and shortening of the estrus time, resulting in a decrease in the conception rate and stillbirth of the cow; the deterioration of the sperm quality of the bull and the decrease in fecundity; it can also reduce the immunoglobulin in the cow. Reduced resistance increases the incidence of obstetric diseases such as mastitis, endometritis, retained placenta, etc....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027C12N15/12C12Q1/68
CPCY02A40/70
Inventor 王根林崔群维闫玉琴蔡亚非
Owner NANJING AGRICULTURAL UNIVERSITY