Fish-in-net immobilized enzyme based on rubricyte carrier

Abstract

The invention relates to a biological/inorganic catalytic reactor with high activity and high stability, namely a fish-in-net immobilized enzyme based on a hematid vector, wherein the catalytic core of the reactor is a biological catalytic material wrapped by hematid; vector hematid is prepared through a method of hypotonicity; a biological material with catalyzing effect is introduced to the vector hematid through a diffusion method; the vector is re-wrapped for subsequent immobilized operation; and the biological-inorganic catalytic reactor is obtained by the immobilized operation, and has a shell made of an inorganic gridding material and the catalytic center made of the biological material wrapped by a hematid membrane. The biological material in the reactor has higher activity and stability, greatly reduces the influence of various organic and inorganic materials in immobilized process on the structure and the activity of the biological material. The reactor can normally construct and execute catalyzing function when the molecular dimension (diameter) of the loaded biological material with catalyzing effect is smaller than 50 nanometers.

Description

technical field [0001] The invention belongs to the fields of inorganic chemistry, physical chemistry and biochemistry, and particularly relates to a biological / inorganic catalytic reactor with high activity and high stability——fish-in-net immobilized enzyme based on red blood cell carrier, the catalytic core of the reactor It is a biocatalytic material encapsulated by red blood cells, and it is an extension and expansion of the "fish-in-net" preparation method. Background technique [0002] Compared with other catalytic materials, biocatalytic materials have the advantages of high efficiency, high specificity and mild reaction conditions. The above advantages make biocatalytic materials have a wide range of application requirements in production and life; however, compared with other catalytic materials, biocatalytic At the same time, the material also has the disadvantages of poor mechanical strength and easy loss of catalytic activity due to environmental factors, and bec...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to use the "fish-in-net" embedding technology based on erythrocyte carrier technology to prepare immobilized biocatalyst - fish-in-net immobilized enzyme based on erythrocyte carrier, the biocatalyst The feature is that the red blood cell carrier is prepared by low dialysis technology, and the target biocatalytic material is encapsulated into the red blood cell carrier through material diffusion. Using the technical method described in our patent 200510017112.9, the surface of the red blood cell is covered with an inorganic shell with a channel structure. Preserve or use a mild denaturant to remove the red blood cell membrane according to the needs of the reaction system, thereby forming a reactor with biocatalytic materials as the catalytic core and an inorganic material with pores as the outer shell. This reactor is suitable for industrial production and application