Fish-in-net immobilized enzyme based on rubricyte carrier
A technology of fish-in-net and immobilized enzymes, which is applied in the direction of immobilization on or in inorganic carriers, immobilization on or in biological cells, etc., to achieve controllable pore size, avoid structure and function damage, and be easy to obtain Effect
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[0022] Example 1: Preparation of embedded galactosidase
[0023] 1) Add 4g of EDTA to 1Kg of fresh beef blood and stir evenly for 30 minutes to chelate calcium ions in the blood and prevent blood agglutination;
[0024] 2) Separate and wash the prepared blood through a hollow cellulose membrane filter with a molecular weight cut-off of 80KD, retain the liquid on the membrane, add isotonic fluid to the system during the washing process, and the composition of the isotonic fluid of red blood cells is 10mMNa 2 HPO 4 / KH 2 PO 4 , 145mM NaCl, 3.5mM KCI and 0.5mM MgCl 2 6H 2 O pH 8.0. Contains 5 mM glucose. During the washing process, the protein concentration in the submembrane solution was detected until the protein concentration was less than 0.01 mg / ml, and the protein concentration was measured by the Coomassie brilliant blue method.
[0025] 3) Add deionized water to the supernatant to reduce the ionic strength of the supernatant to about 10% of its original value, resul...
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