Medical device with cell-regeneration reinforcing function and manufacture method thereof
A technology for medical equipment and sutures, applied in medical science, surgery, surgical instruments, etc., can solve the problems of complex design and difficult commercialization of technology
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Embodiment 1
[0059] Embodiment 1 separates and purifies dulcitol from dulcis
[0060] Separate the leaves, stems and roots of euonymus, wash with water, chop finely, and dry in a cool place until there is no change in weight. Each part of 1 kg of dried euonymus was added to 1 liter of ethanol, extracted at 60° C. for 8 hours, and filtered with gauze. The ethanol was then completely evaporated using a rotary vacuum evaporator (Heidolph WB2000, Germany), thereby obtaining fractions of extracts.
[0061] 200 ml of distilled water was added to the extracts of each fraction and fractionated using diethyl ether. Then, the fractionated aqueous layer was concentrated and fractionated using ethyl acetate to obtain an organic solvent layer. The organic solvent layer was treated by freeze-drying, thereby obtaining the extracted powder of each fraction.
[0062] The extracted powder of each part was dissolved in 100 ml of distilled water, and then separated by silica gel column chromatography, usin...
Embodiment 2-7
[0065] Examples 2-7: Preparation of Sutures with Different Collagen Contents
[0066] Bovine-derived collagen was dissolved in pure water at 50° C. to prepare collagen coating solutions having 30, 40, 50, 60, 70 and 80% by weight of collagen, respectively. The spools wound with polydioxanone monofilaments were soaked in the collagen coating solution for 30 min, stirred and dried at 22 °C to obtain collagen-coated polydioxanone sutures.
[0067] The dulcitol of Example 1 was dissolved in pure water at 50° C. to prepare a dulcitol coating solution having 10% by weight of dulcitol. The spools of wound polydioxanone monofilaments coated with collagen were soaked in dulcitol coating solution for 30 min, stirred and dried at 22 °C to obtain collagen and dulcitol-coated polydioxanone monofilaments. Oxanone sutures.
Embodiment 8-12
[0068] Examples 8-12: Preparation of sutures with different dulcitol contents
[0069] A collagen coating solution having 50% by weight of collagen was prepared by dissolving bovine-derived collagen in pure water at 50°C. The bobbins wound with polydioxanone monofilaments were soaked in the collagen coating solution for 30 minutes, stirred and dried at 22° C. to obtain collagen-coated polydioxanone sutures.
[0070] The dulcitol of Example 1 was dissolved in pure water at 50° C. to prepare dulcitol coating solutions having 3, 5, 10, 15 and 20% by weight of dulcitol, respectively. A spool of wound polydioxanone suture coated with collagen was soaked in dulcitol coating solution for 30 min, stirred and dried at 22 °C to obtain collagen and dulcitol-coated polydioxan Alkanone sutures.
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