Uridine diphosphate-4-one-6-deoxyglucose heterogeneous reductase and coding gene thereof and application

A uridine diphosphate rhamnose and gene technology, applied in the fields of oxidoreductase, application, genetic engineering, etc., can solve the problem of losing the stomata opening and closing function of isolated Commelina leaves, and achieve great economic value and application prospects , fiber length increase, the effect of improving quality and yield

Inactive Publication Date: 2009-06-10
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2003, Jones et al. used arabinanase to hydrolyze pectin RG I to lose

Method used

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  • Uridine diphosphate-4-one-6-deoxyglucose heterogeneous reductase and coding gene thereof and application
  • Uridine diphosphate-4-one-6-deoxyglucose heterogeneous reductase and coding gene thereof and application
  • Uridine diphosphate-4-one-6-deoxyglucose heterogeneous reductase and coding gene thereof and application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1. Cloning of the gene encoding uridine diphosphate-4-keto-6-deoxyglucose isomeroreductase

[0046] 1. Cloning of GhUER1 gene

[0047] Select wild-type upland cotton variety Xuzhou 142 (WT) (National Mid-term Cotton Seed Bank of Cotton Research Institute, Chinese Academy of Agricultural Sciences) fibers 10 days after flowering, extract total RNA, and extract total RNA according to the RNA gentsTotal RNA Isolation System kit of Promega Company conduct. Take 5g of total RNA and reverse transcribe to obtain one strand of cDNA. The reaction system is as follows:

[0048] Total RNA 10 μL (5 μg), 0ligo (dT) (20 μmol / L) 1.5 μL, 10× buffer 2.5 μL, dNTPmix (2.5 mmol / L) 2 μL, sterile water 8 μL.

[0049] Add 1 μL (200U) SuperScript to the reaction system after bathing in water at 42°C for 1 min TM IIRT, mixed gently, incubated at 42°C for 50min; bathed in water at 70°C for 15min, terminated the reaction; obtained the first strand of cDNA.

[0050] Primers were design...

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Abstract

The invention discloses uridine diphosphate-4-ketone-6-deoxyglucose isomerous reductase, and encoding genes and application thereof. The uridine diphosphate-4-ketone-6-deoxyglucose isomerous reductase is a) or b): a), proteins which are formed by amino acid sequences shown in a sequence 2 in a sequence table; and b), proteins which are formed by the amino acid sequences shown in the sequence 2 in the sequence table subjected to substitution and/or deletion and/or addition of one or more than one amino acid, related to synthesis of uridine diphosphate rhamnose and derived from the a). The invention also discloses the encoding genes for the proteins. After the encoding genes of the uridine diphosphate-4-ketone-6-deoxyglucose isomerous reductase are transferred into cotton, the fiber length of the cotton is increased and the quality and the yield of cotton fibers are improved. The uridine diphosphate-4-ketone-6-deoxyglucose isomerous reductase and the encoding genes of the uridine diphosphate-4-ketone-6-deoxyglucose isomerous reductase have large economic value and application prospect.

Description

technical field [0001] The invention relates to uridine diphosphate-4-keto-6-deoxyglucose isomeroreductase, its coding gene and application. Background technique [0002] Cotton fiber is a unicellular structure differentiated from the outer skin cells of the ovule. Cotton fiber is an important raw material for the textile industry and has important economic value. Fiber quality determines its final length and strength. At the same time, cotton fiber cells are also an ideal system for studying important biological phenomena such as cell elongation, differentiation and cell wall synthesis. Therefore, the study of fiber elongation has important economic value and theoretical significance. [0003] The development process of cotton fiber cells is a process of supernormal cell elongation and supernormal cell wall thickening. The length of upland cotton is about 3.0cm, and the diameter of the upland cotton cell wall is 11-22μm, that is to say, the aspect ratio is 1000-3000. C...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N15/53C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 朱玉贤庞朝友逄宇王慧靳翔秦咏梅
Owner PEKING UNIV
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