Progestational hormone progesterone measuring method in cosmetics
A method for measuring progesterone and a technology for progesterone, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of inaccurate qualitative and micro-quantification, and achieve the effect of reliable implementation and easy implementation.
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Embodiment 1
[0043] Example 1, the precision and recovery detection of progesterone progesterone in solution cosmetics:
[0044] The sample to be tested is a solution sample. Accurately weigh 1.000g~2.000g (±0.01g) of the fully mixed sample into a 10mL stoppered colorimetric tube, distill off volatile organic solvents such as ethanol on a water bath, and use Dilute methanol to 10mL, mix well, pass through a 0.22μm filter membrane, and use it as a sample treatment solution for later use.
[0045] Progesterone standard stock solution: Weigh progesterone 0.01g (accurate to 0.0001g) in a 100ml volumetric flask, dissolve with a small amount of methanol, and finally dilute to the mark with methanol. This solution is used as progesterone standard stock solution. 1mL of this solution contains 0.1mg of progesterone, and then diluted to 10.0, 50.0, 100, 200, 500ng / mL standard solution.
[0046] The detection conditions are:
[0047] Liquid phase conditions:
[0048] Chromatographic column: ACQUIT...
Embodiment 2
[0076] Embodiment 2, the detection of progesterone progesterone precision and recovery rate in creamy, milky cosmetics:
[0077] The sample to be tested is a creamy or milky sample. Accurately weigh 1.000g-2.000g (±0.01g) of the fully mixed sample into a 100mL conical flask, distill off ethanol and other volatile organic solvents on a water bath, add Saturated sodium chloride solution 50mL, 2% sulfuric acid 2.0mL, fully shake to dissolve, transfer to 250mL separatory funnel, extract three times with 30mL cyclohexane, centrifuge if necessary, combine cyclohexane, remove cyclohexane by vacuum distillation , dissolve the residue in methanol, transfer to a 10mL stoppered colorimetric tube, and dilute to the mark with methanol. After fully mixing, pass through a 0.22 μm filter membrane and use it as a sample treatment solution for later use.
[0078] Weigh 0.01g of progesterone (accurate to 0.0001g) in a 100ml volumetric flask, dissolve with a small amount of methanol, and finally d...
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