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Klebsiella pneumoniae and its application in preparing 2,3-butanediol

A technology of Klebsiella and butanediol is applied in the field of bioengineering to achieve the effects of high product concentration and short fermentation period

Active Publication Date: 2009-06-17
上海肆芃科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, research and development of high-concentration 2,3-butanediol fermentation production methods and special bacteria have important practical significance. After searching, there is no use of Klebsiella pneumoniae to ferment high-yield 2,3-butanediol. patent coverage

Method used

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  • Klebsiella pneumoniae and its application in preparing 2,3-butanediol

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Experimental program
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Effect test

Embodiment 1

[0039] Example 1: Separation, mutagenesis screening and identification of Klebsiella pneumoniae SDM CCTCC M 208097

[0040] The composition of the medium used in this embodiment is as follows:

[0041] Nutrient liquid medium: glucose 140g / L, yeast powder 10g / L, peptone 10g / L, sodium chloride 5g / L, pH 6.

[0042] Nutrient agar medium: glucose 50g / L, yeast powder 10g / L, peptone 10g / L, sodium chloride 5g / L, pH 6.

[0043] Fermentation medium: glucose 140g / L, yeast powder 10g / L, peptone 10g / L, sodium chloride 5g / L, pH 6.

[0044] Nutrient broth medium: glucose 50g / L, yeast powder 10g / L, peptone 10g / L, sodium chloride 5g / L, pH 6.

[0045] The specific operation process of this embodiment is as follows:

[0046] 1. Separation and mutagenesis screening

[0047] The humus soil layer was obtained from the orchard, and every 1 g was added to a 300 mL Erlenmeyer flask (50 mL of sterilized nutrient liquid medium was housed), and placed on a shaker at a speed of 180 rpm for 48 hours at...

Embodiment 2

[0052] Embodiment 2: Utilize Klebsiella pneumoniae (Klebsiella pneumoniae) SDM CCTCC M 208097 to ferment and produce 2,3-butanediol in Erlenmeyer flask

[0053] The sequence of steps involved in applying the method is as follows:

[0054] (1) Strain selection: select Klebsiella pneumoniae (Klebsiellapneumoniae) SDM CCTCC M208097;

[0055] (2) Slant culture: inoculate the strains on the slant medium, and culture at 37°C for 12 hours;

[0056] (3) Seed cultivation: the bacterial strain cultivated in step (2) was placed in a 300mL Erlenmeyer flask with 50mL seed medium under aseptic conditions with an inoculation loop, and cultured on a shaker at 37°C for 10 hours. Rotating speed 150rpm, makes seed liquid;

[0057] (4) Fermentation culture: insert 10mL of the seed culture liquid of step (3) in the 500mL Erlenmeyer flask that 100mL fermentation medium is housed, under 37 ℃ of conditions, shake table culture 16 hours, finish fermentation, the rotating speed of this shake table is...

Embodiment 3

[0063] Embodiment 3: Utilize Klebsiella pneumoniae (Klebsiella pneumoniae) SDM CCTCC M 208097 to ferment and produce 2,3-butanediol in Erlenmeyer flask

[0064] The sequence of steps involved in applying the method is as follows:

[0065](1) Strain selection: select Klebsiella pneumoniae (Klebsiella pneumoniae) SDM CCTCC M 208097;

[0066] (2) Slant culture: inoculate the strains on the slant medium, and culture at 40°C for 14 hours;

[0067] (3) Seed cultivation: the bacterial strain cultivated in step (2) was placed in a 300mL Erlenmeyer flask containing 50mL of seed medium with an inoculation loop under aseptic conditions, and cultured on a shaker at 40°C for 11 hours. Rotating speed 150rpm, makes seed liquid;

[0068] (4) Fermentation culture: 10 mL of the seed culture solution of step (3) was inserted into a 500 mL Erlenmeyer flask containing 100 mL of fermentation medium, and cultured on a shaking table at 40° C. for 13 hours to end the fermentation. The rotation spee...

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Abstract

The invention discloses a Klebsiella pneumoniae SDM CCTCC M 208097 and applications in preparing 2, 3-butanediol. The Klebsiella pneumoniae SDM CCTCC M 208097 has features of wide nutritional requirement range, high outcome concentration and short production period or the like. The culture medium and the operation method are simple, the cost is low. The outcome of the 2, 3-butanediol prepared by a fermenter feeding batch fermentation can reach 120-160g / L, the conversion can reach 90-940f the theoretic conversion, the most production rate of the 2, 3-butanediol is 4. 05g / (L. h). The invention has very large commercial use prospect.

Description

technical field [0001] The invention relates to a strain of Klebsiella pneumoniae and its application, in particular to a strain of Klebsiella pneumoniae with high yield of 2,3-butanediol and its application in the preparation of 2,3-butanediol, belonging to The field of bioengineering technology. Background technique [0002] 2,3-butanediol (2,3-butanediol), also known as 2,3-dihydroxybutane, is a colorless and odorless liquid, widely used in medicine, chemical industry, food, fuel and aerospace, etc. Various fields (Xiu ZL, Zeng AP. Appl Microbiol Biotechnol, 2008, DOI 10.1007 / s00253-008-1387-4). 2,3-butanediol can be used as a precursor for the production of methyl ethyl ketone and 1,3-butadiene, which are widely used in the chemical industry. The combustion value of 2,3-butanediol is 27200kJ / kg, which is equivalent to that of ethanol (29055kJ / kg), and it is a fuel additive with high potential value. 2,3-Butanediol can be condensed with methyl ethyl ketone and hydrogen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/18C12R1/22
Inventor 马翠卿王爱龙许平
Owner 上海肆芃科技有限公司
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