Flavone active site of Vernonia cinerea as well as preparation method and use thereof
An active part, the technology of nightshade, applied in the field of natural medicinal chemistry
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Embodiment 1
[0011] Embodiment 1: the preparation method of flavonoid active site
[0012] Take by weighing 5.0Kg of the whole herb of Ye Xiangniu as a raw material, dry and pulverize it and extract 3 times with an ethanol solution with a volume percentage concentration of 70%, and the quality of the ethanol solution used for the three times is successively 10 times, 8 times that of the whole herb of Ye Xiangniu. times and 8 times, each extraction for 2 hours, the three extracts were filtered with gauze and combined; the combined extracts were concentrated under reduced pressure at a temperature lower than 80°C to recover ethanol until there was no alcohol smell, and ethanol extraction was obtained substance; the ethanol extract was diluted with distilled water to a density of 1.10-1.20g / cm 3 , followed by extraction with analytically pure petroleum ether with a boiling range of 60-90°C for 5 times, analytically pure ethyl acetate for 5 times, and analytically pure water for saturated n-bu...
Embodiment 2
[0013] Example 2: Structural Identification of Flavonoid Active Sites
[0014] 40g of the flavonoid active fraction of Yexiangniu, mix the sample with silica gel (0.063-0.200mm, 250g), and chromatographically separate on a silica gel column (0.060-0.200mm), and use dichloromethane-methanol (100:0-100:30) gradient Elution, each 100mL as a fraction, until 105 parts in total; dichloromethane-methanol (100:8) part was separated by silica gel column (0.040-0.063mm) and Sephadex LH-20 column chromatography to obtain compound I (15mg) , II (15mg); Dichloromethane-methanol (100:10) was partly separated by silica gel column (0.040-0.063mm) and SephadexLH-20 column chromatography to obtain compound III (18mg), VI (20mg); Dichloromethane - Methanol (100:20) was separated by repeated column chromatography on silica gel (0.040-0.063mm) and Sephadex LH-20 column chromatography to obtain compounds IV (20 mg), V (15 mg), and VII (25 mg).
[0015] Table 1 13 C-NMR (100MHz, DMSO-d 6, δppm) d...
Embodiment 3
[0017] Example 3: Preliminary research data on the anti-senile dementia effect of flavonoid active fractions
[0018] The ethyl acetate extract, the elution site of the macroporous resin, and compounds I-VII were screened for nerve cell activity. The results showed that both the ethyl acetate extract and the elution site of the macroporous resin had strong NGF-inducing activity, and compound I -VII has certain NGF-inducing activity.
[0019] 1. Principle and method
[0020] The NGF-inducing activity of the monomeric compound was detected by the in vitro model test of nerve cells and PC-12 cells.
[0021] (1) PC 12 cell line is a cell line differentiated from rat adrenal medullary pheochromocytoma, which has the general characteristics of neuroendocrine cells, and is now widely used in neurophysiological and neuropharmacological research.
[0022] When cultured with very low concentration of NGF, PC 12 cells can be induced to differentiate into NGF-dependent dopaminergic neur...
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