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High-performance liquid chromatography analysis method for oryzanol

A high-performance liquid chromatography, oryzanol technology, applied in the direction of analysis materials, material separation, measuring devices, etc., can solve the problems of low separation, poor separation, affecting the quality of oryzanol, etc.

Active Publication Date: 2009-07-15
BEIJING CENTURY BIOCOM PHARMA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology has certain defects in the detection and analysis of oryzanol component content, often only four or five components can be detected, and the separation degree is not high, which affects the quality of oryzanol
For example, in the review literature: The influence of crude rice bran oil refining on the content of oryzanol (Sichuan Grain and Oil Science and Technology: 2002.76: 13-16), the oryzanol liquid phase analysis spectrum obtained by the existing high performance liquid phase analysis method is given, which only About five substances can be distinguished, and the resolution of the latter three is obviously poor

Method used

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  • High-performance liquid chromatography analysis method for oryzanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The selection of embodiment 1 chromatographic conditions

[0048] 1.1 Detection of absorption wavelength

[0049] Continuously scan the oryzanol raw material sample, cycloartenol ferulate reference substance, and 24-methylene cycloartenol ferulate reference substance within the wavelength of 400nm-200nm with an ultraviolet spectrophotometer, and observe the figure 1 , 2 3. It can be found that the maximum absorption wavelength is 325nm, so the present invention selects the detection wavelength as 325nm.

[0050] 1.2 Sample concentration

[0051] In order to take into account the determination of main components and impurity content, an appropriate sample concentration must be selected. If the concentration is too small, it is not conducive to the peak of impurities, and if it is too large, it will affect the main peak beyond the range of the bee line. After comprehensive measurement and with reference to the existing experimental data, the sample solution was select...

Embodiment 2

[0054] Embodiment 2 Configuration of reference substance solution

[0055]Precisely weigh an appropriate amount of cycloarneol ferulic acid ester reference substance and 24-methylene cycloarneol ferulic acid ester reference substance, and add mobile phase A solution to make cycloartenol ferulic acid per ml Ester reference substance and 0.5mg solution of 24-methylenecycloartenol ferulic acid ester, accurately measure the appropriate amount of the solution, add mobile phase A solution to make each ml containing cycloarneol ferulic acid Each 25, 50, 125, 250, and 500 μg solution of the ester reference substance and 24-methylenecycloartenyl ferulic acid ester was taken respectively and 10 μl of each solution was injected into the liquid chromatograph, and the peak areas under each concentration were recorded. And make a linear regression with the peak area A to the concentration C (μg / ml).

Embodiment 3

[0056] Embodiment 3 Determination of Standard Curve

[0057] The experiment was carried out on the basis of Example 2, and the peak area was plotted against the concentration.

[0058] The obtained test data of cycloartenol ferulic acid ester reference substance is as follows

[0059] Table 3 Liquid phase analysis data of cycloartenol ferulate

[0060]

[0061] The experimental data obtained by the 24-methylenecycloartenyl ferulic acid ester reference substance is as follows

[0062] Table 4 24-methylenecycloartenol ferulic acid ester liquid phase analysis data

[0063]

[0064] From the above results, it can be seen that the peak area A of cycloartenol ferulate has a linear relationship with the concentration C in the concentration range of 26.3-525.0 μg / ml, and the concentration of 24-methylene cycloartenol ferulate In the concentration range of 21.3~475.0μg / ml, the peak area A and the concentration C have a linear relationship. Figure 4 , 5 .

[0065] Based on ...

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Abstract

The invention discloses a high-efficiency HPLC analysis method of oryzanol, belonging to the analytical chemistry field. The contents of cycloartenyl ferulate and 24-methylene-cycloartenyl ferulate in the oryzanol, oryzanol extraction separation and purification product, oryzanol medicine can be quickly and accurately analyzed using the HPLC analysis method and at the same time the contents of the other impurities can be analyzed. The high-efficiency HPLC analysis method can also be used for the oryzanol medicine quality research such as oryzanol medicine stability, breakage or the like.

Description

technical field [0001] The invention discloses a high performance liquid chromatography analysis method for oryzanol, which belongs to the field of analytical chemistry. Background technique [0002] Oryzanol is a natural mixture composed of cycloartenol-based ferulic acid esters and sterol-based ferulic acid esters. The appearance is white to light yellow crystalline powder, odorless. Oryzanol mainly exists in hair bran oil and its oil feet. In rice bran oil oryzanol, the content of cycloartenol ferulate is about 70-80%. After long-term research, researchers have found that oryzanol has a variety of physiological functions. Mainly include: lowering blood lipids, resisting the absorption of cholesterol, lowering serum cholesterol, preventing lipid oxidation and preventing cardiovascular diseases. In addition, oryzanol can also alleviate various physical disorders and autonomic nervous disorders in women after menopause, and improve the dysfunction of the base of the dience...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34
Inventor 何乐张金海郝守祝
Owner BEIJING CENTURY BIOCOM PHARMA TECH
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