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Fast extraction and measurement method for plastid pigment in tobacco and tobacco products

A tobacco product and measurement method technology, applied in the field of tobacco chemical determination, can solve the problems of poor stability of carotenoid pigments, long extraction time, complicated pretreatment, etc., achieve shortened pretreatment time, high accuracy of measurement results, and easy operation Simple and easy-to-control effects

Inactive Publication Date: 2009-07-22
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chlorophyll and carotenoid pigments in plastid pigments are poorly stable, and require low temperature, dark, and fast extraction and measurement.
And the extraction of existing plastid pigment is commonly used vibration extraction method etc., and the vibration extraction method extraction needs longer time, easily causes the chlorophyll in the plastid pigment and carotenoid pigment oxidative decomposition; Determination often adopts spectrophotometry, High-efficiency thin-layer chromatography scanning method and liquid chromatography, the accuracy of spectrophotometry is relatively poor, high-efficiency thin-layer chromatography scanning method and traditional liquid chromatography sample pretreatment are complicated and inefficient, although solid-phase extraction can be used Simplifies sample preparation, but is more expensive to use

Method used

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  • Fast extraction and measurement method for plastid pigment in tobacco and tobacco products
  • Fast extraction and measurement method for plastid pigment in tobacco and tobacco products

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Take 500g of cured tobacco leaf sample according to the sample sampling standard, dry it at 40°C for 4h, crush it, pass it through a 40 mesh sieve, and the sample preparation is completed; accurately weigh 5.0000g of the prepared sample (accurate to 0.0001g), and pour it into 100ml Erlenmeyer flask, pour 40ml of 90% acetone (containing 0.1% dibutyl hydroxytoluene BHT) into the Erlenmeyer flask, ultrasonic extraction at room temperature for 20min, filter, and then use 10ml of 90% acetone (containing 0.1% dibutyl hydroxytoluene BHT) for the filter residue Hydroxyl toluene (BHT) was washed 2-3 times, the filtrate was combined, and the volume was adjusted to 100ml, and 2mL of the extract was taken, filtered with a 0.45μm microporous membrane, and the filtrate was analyzed by HPLC and quantified by the external standard method; wherein, the chromatographic column was SymmetryC18 Phase chromatographic column (3.9mm i.d. × 150mm, 4 μm) (Waters Company, USA); Mobile phase is: A,...

Embodiment 2

[0022] According to the sample sampling standard, take 500g of finished cigarette samples, dry them at 40°C for 4 hours, crush them, pass through a 40-mesh sieve, and the sample preparation is completed; accurately weigh 5.0000g of the prepared samples (accurate to 0.0001g), and pour them into a 100ml triangular bottle, pour 40ml of 90% acetone (containing 0.1% dibutylhydroxytoluene BHT) into the conical flask, ultrasonically extract at room temperature for 20min, filter, and then use 10ml of 90% acetone (containing 0.1% dibutyl hydroxytoluene BHT) to filter the residue Hydroxytoluene (BHT) was washed 2 to 3 times, the filtrate was combined, and the volume was adjusted to 100ml, and 2mL of the extract was taken, filtered with a 0.45 μm microporous membrane, and the filtrate was analyzed by HPLC and quantified by the external standard method; the chromatographic column was a SymmetryC18 reverse-phase chromatographic column (3.9mm i.d.×150mm, 4μm) (Waters, USA); mobile phase: A, ...

Embodiment 3

[0024] Take 3000g of fresh tobacco leaves from the field, freeze-dry them at -45°C, crush them, pass through a 40-mesh sieve, and the sample preparation is completed; accurately weigh 1.0000g of the prepared sample (accurate to 0.0001g), pour it into a 100ml conical flask, and use 40ml90 % acetone (containing 0.1% dibutyl hydroxytoluene BHT) was poured into a conical flask, ultrasonically extracted at room temperature for 20 min, filtered, and the filter residue was washed with 10 ml of 90% acetone (containing 0.1% dibutyl hydroxytoluene BHT) for 2 ~ 3 times, combine the filtrate, dilute to 100ml, take 2mL extract, filter with 0.45μm microporous membrane, carry out HPLC analysis on the filtrate, quantify by external standard method; chromatographic column is SymmetryC18 reversed-phase chromatographic column (3.9mm i.d.×150mm , 4 μm) (U.S. Waters Company); Mobile phase is: A, 80% acetonitrile: isopropanol=1:1, B, ultrapure water; Flow velocity is 0.5ml / min, gradient elution; Det...

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Abstract

The invention relates to a method for rapidly extracting and measuring plastid pigment in tobacco and tobacco products, comprising the following steps: (1) a method of ultrasonic extraction is adopted to rapidly extract the plastid pigment in tobacco and tobacco products; (2) after ultrasonic extraction, the content of all the components in the extracted plastid pigment is measured by high performance liquid chromatography. The invention adopts the high performance liquid chromatography to rapidly measure the extracted plastid pigment, and the content of all the components in the plastid pigment can be rapidly and exactly measured, thus greatly improving the measuring accuracy. By adopting the extracting and measuring method, the working efficiency is greatly improved, and the cost is also reduced. Therefore, the invention has good social and economic values, and is easy to popularize and apply.

Description

technical field [0001] The invention relates to a method for rapidly extracting and measuring plastid pigments in tobacco and tobacco products, in particular to a method for rapidly extracting plastid pigments in tobacco and tobacco products by ultrasonic extraction, and measuring the plastid pigments in tobacco The invention belongs to the field of tobacco chemical determination method. Background technique [0002] There are many components of plastid pigments in tobacco and tobacco products, mainly including chlorophyll a, chlorophyll b, β-carotene, lutein, neoxanthin, violaxanthin, etc. These components are the main components that affect the aroma quality and usability of flue-cured tobacco. If the tobacco leaves are completely and properly degraded in the process of brewing, processing and aging, the aroma of the tobacco leaves will be increased, the color will be bright, and the quality and usability of the tobacco leaves will be improved. The establishment of a rapi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
Inventor 李世勇韦凤杰田海英张东豫张弘韬刘蕙芳
Owner CHINA TOBACCO HENAN IND
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