Antimicrobial micelles for food applications
An antimicrobial agent and food technology, applied in the field of food-grade micelles, can solve problems such as changes in food taste
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[0051] The emulsion is prepared by mixing a food-grade surfactant in an aqueous medium to form micelles, and then adding an antimicrobial agent to the emulsion of micelles. Preferably, the antimicrobial is added with agitation over a period of time, usually about 10-15 minutes.
[0052] Preferably the antimicrobial agent is selected from clove oil, bay oil, cinnamon oil, oregano oil, thyme oil, caraway oil, dill oil, coriander oil, lemon oil, orange peel oil, mustard oil or any combination of these. More preferred is clove oil, oregano oil, mustard oil or any combination of these.
[0053] The amount of antimicrobial substance to be added can easily be determined by measuring turbidity. In fact, antimicrobial agents are dispersed in water by encapsulation in micelles. When turbidity occurs in the aqueous solution, it means that the antimicrobial agent that is poorly soluble or insoluble in water is no longer incorporated into the micelles. Typically, the concentration of an...
Embodiment 1a
[0070] Example 1a: In vitro test
[0071] Media used:
[0072] -M.R.S.-Broth, Merck, 1.10661
[0073] -2% Viridian bile salt (broth), Oxoid company, CM0031
[0074] - Peptone from pancreatically digested casein, Merck, 1.07213
[0075] -D(+)-glucose, Merck, 1.08337
[0076] Agar used:
[0077] -M.R.S. Agar, Oxoid Corporation, CM 0316
[0078] - Tryptone Bile X-Glucuronide Agar (TBX), Merck, 1.16122 Emulsifier used:
[0079] -Surfynol 495W, Air Products Nederland BV, S-485W
[0080] -Admul T 80K, Kerry Bio-Science, 5Z10754 Essential oil composition used:
[0081] - Eugenol, Fluka, 41600
[0082] - Carvacrol, Fruka Corporation, 22051
[0083] -R(+)-limonene, Veruca, 62119
[0084] Microorganisms used:
[0085] -Lactobacillus brucelli DSM 20174 418 * / #
[0086] - Escherichia coli SLV 082 504 *
[0087] All emulsions were prepared with distilled deionized water.
[0088] "Empty" micelles are prepared by mixing an emulsifier and water.
[0089] The goal is to enca...
Embodiment 1b-1d
[0108] The antimicrobial properties of micelles encapsulating essential oils, particularly clove oil, oregano oil and mustard oil, were tested using the same in vitro conditions as above.
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