Method for identifying pleurotus citrinopilestus by using molecular marker technology

A technology of Pleurotus aureus and molecular markers, which is applied to biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of little research on the identification of Pleurotus aureus species, and achieve short detection time, easy judgment, and high sensitivity. high effect

Inactive Publication Date: 2009-08-19
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0006] Although scholars have done more research on the cultivation, nutritional components and efficacy of Pleurotus aureus, there are few studies on the identification of Pleurotus a

Method used

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  • Method for identifying pleurotus citrinopilestus by using molecular marker technology
  • Method for identifying pleurotus citrinopilestus by using molecular marker technology
  • Method for identifying pleurotus citrinopilestus by using molecular marker technology

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Embodiment 1

[0022] Example 1: A method for identifying Pleurotus aureus in Jiayu by using molecular marker technology

[0023] A method for identifying Pleurotus aureus in Jiayu by molecular marker technology, comprising the following steps:

[0024] 1. Mycelia culture and collection

[0025] (1) If the preservation time of the Pleurotus aureus strain for testing is less than 6 months, the mycelia culture shall be carried out as follows;

[0026] 1. Use an inoculation rake to crush the PDA medium containing Pleurotus aureus mycelia, and transfer it to a 250ml Erlenmeyer flask containing 100ml PDA liquid medium;

[0027] 2. Place on a shaker at 25°C with a rotation speed of 90-130r / min and cultivate for 7-10 days;

[0028] 3. Filter the cultured mycelia with gauze, rinse with distilled water, and dry with filter paper;

[0029] 4. Weigh 0.5-1.3 g of mycelium, wrap it with filter paper, and store it at -20°C for later use.

[0030] (2) If the Pleurotus aureus strain to be tested has bee...

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Abstract

A method which uses a molecular marker technology for identifying Pleurotus citrinopileatus comprises the cultivation and collection of mycelium, the extraction of genome DNA, the establishment of the detection of a SCAR-PCR molecular marker, and the detection of a SCAR-PCR product. The method has the advantages of high sensitivity, less usage of needed DNA, and short detection time, high accuracy, easy judgment, good reproducibility, and the like, compared with conventional morphology detection, antagonistic test, and fruiting test. The method provides an effective strain identification method for the use of Pleurotus citrinopileatus germplasm resources and the registration work of new varieties.

Description

technical field [0001] The invention relates to a method for identifying microorganisms, in particular to a method for identifying Pleurotus aureus by using molecular marker technology, and belongs to the field of biotechnology. Background technique [0002] Pleurotus citrinopileatus Singer, also known as Pleurotus citrinopileatus Singer, is a unique edible and medicinal fungus in Northeast China. Pleurotus chinensis not only has delicious taste, strong fragrance and rich nutritional value, but also has medicinal value of nourishing and strengthening the body. It can be used to treat impotence and dysentery due to kidney deficiency. Pleurotus aureus contains polysaccharides, rich amino acids, proteins, vitamins, minerals and other nutrients. It is a high-nutrition, low-calorie food and is known as "vegetarian meat". Extracted polysaccharides have inhibitory effect on human colon poorly differentiated adenocarcinoma cells. Through the analysis of its chemical composition, i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 熊芳温志强谢宝贵刘新锐吴小平
Owner FUJIAN AGRI & FORESTRY UNIV
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