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Method for improving reproductive speed of microbial strain

A technology of microbial strains and speed, applied in the field of microorganisms, can solve the problems of reducing the efficiency of decomposing organic matter and increasing microbial strains, so as to achieve the effect of increasing efficiency, increasing the speed of reproduction, and better decomposing organic waste

Active Publication Date: 2013-01-02
TANTZ ENVIRONMENTAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a microbial strain that can rapidly multiply in a short time in order to solve the problems of inhibiting microbial activity and reducing the efficiency of decomposing organic matter in the prior art when directly adding microbial strains to organic waste for biodegradation. , a method for significantly increasing the number of microbial strains to increase the reproduction rate of microbial strains

Method used

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  • Method for improving reproductive speed of microbial strain
  • Method for improving reproductive speed of microbial strain
  • Method for improving reproductive speed of microbial strain

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1 Microbial strains

[0039]The microbial strain selection of the present embodiment has the bacterial classification that has registration number in American Type Culture Center (American TypeCulture Centre, ATCC) and the bacterial classification on Canadian Civil Substance List (Canadian Domestic Substance List, DSL), with To ensure its safety, it specifically includes the following strains: Bacillus amyloliquefaciens ATCC14579, Bacillus cereus ATCC14579, Bacillus circulans ATCC9500, Bacillus licheniformis ATCC 12713, Bacillus megaterium ATCC 14581, Bacillus polymyxa ATCC 842, Bacillus subtilis ATCC 6051, Pseudomonas fluorescens ATCC 13525 and Pseudomonas putida ( Pseudomonas Putida) ATCC 12633.

[0040] The microbial strains of the present embodiment take wheat bran and seaweed as carriers, and the total number of colonies is 1×10 8 ~1×10 10 cfu / g.

Embodiment 2

[0041] Example 2 Propagation of microbial strains under the conditions of containing Yucca extract and not containing Yucca extract respectively

[0042] In this embodiment, the yucca extract and humic acid are mixed together with the microbial strains in Example 1 for aerobic treatment. The yucca extract (wherein the steroid saponin mass percentage is 38.0%) is commercially available.

[0043] The specific operation steps of this embodiment are as follows:

[0044] (1) Weigh 10 mL of Yucca extract (wherein the steroid saponin is 3.8 g) and 3 g of humic acid are added to a 1000 mL beaker, then 990 mL of deionized water is added, and stirred evenly;

[0045] (2) Add the microbial strain of 10g embodiment 1 in the above-mentioned mixed solution, fully stir, and record as experimental group;

[0046] (3) Get another 1000mL beaker, add 1000mL deionized water to the inside, then add the microbial strain of 10g embodiment 1, fully stir, and record as the control group;

[0047] (4...

Embodiment 3

[0055] Embodiment 3 The reproduction of microbial strains under aerobic / anaerobic conditions respectively

[0056] The present embodiment is divided into experimental group and control group, and experimental group adopts the experimental group prepared in embodiment 2, and its aerobic treatment is also with embodiment 2, and the preparation of control group is as follows:

[0057] (1) Weigh 10 mL of Yucca extract (wherein the steroid saponin is 3.8 g) and 3 g of humic acid are added to a 1000 mL beaker, then 990 mL of deionized water is added, and stirred evenly;

[0058] (2) Add the microbial strain of 10g embodiment 1 in above-mentioned mixed solution, fully stir, and record as control group;

[0059] (3) After the control group was stirred evenly, no aerobic treatment was carried out, and in the 24 hours when the experimental group was aerated, the control group remained in a static state;

[0060] (4) During the 24 hours when the experimental group was aerated, samples w...

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Abstract

The invention discloses a method for improving reproductive speed of microbial strains. In the method, steroid saponin is added to and well mixed with the microbial strains for aerobic treatment. A preferred embodiment of the invention comprises the following steps: adding yucca extracting solution and humic acid to the microbial strains, then fully mixing under aerobic conditions (aeration / stirring) for 3-5h, and directly adding the microbial strains to a facility for organic waste disposal after the aerobic treatment, or directly to organic wastes for organic waste decomposition. In the method, the steroid saponin and the humic acid are added to the microbial strains, which increases growth and reproductive speed of the microbial strains during the aerobic treatment, increases total bacterial count and enhances organic waste decomposition efficiency, and ensures that the treated strains can be directly used without significant decrease in metabolic activities such as activation, reproduction and the like. In addition, the method requires low-cost raw materials and simple operation, and is favorable for large-scale popularization.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for increasing the propagation speed of microorganisms. Background technique [0002] Microbial technology is an applied technology that combines the principles of biology and engineering. It extracts microbial strains from soil or water in nature, passes various rigorous tests in the laboratory to determine their ability to decompose various organic matter, and then selects strains with practical uses for cultivation and propagation, and reaches commercial scale. application to solve a specific problem. Each strain can produce one or several kinds of biological enzymes that degrade a certain type of organic matter, and different types of strains work together to effectively decompose complex organic matter into simple small molecules, and finally into inorganic Toxic and harmless products such as water and carbon dioxide. [0003] There have been a large numbe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12R1/00
Inventor 吴小明许瑛华刘宏韦勇强王朝霞陈文雄潘志添
Owner TANTZ ENVIRONMENTAL TECH
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