Label-free biochemical detection method reinforced by utilizing local surface plasma

A plasma and localized surface technology, applied in the field of biochemical detection, can solve the problems of low sensitivity, high cost, long cycle, etc., and achieve the effect of high sensitivity, low cost and good bioaffinity

Inactive Publication Date: 2009-08-26
INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

[0004] The problem to be solved by the present invention is: to overcome the shortcomings of existing biochemical sensing technology that require special equipment, high cost, long cycle, and low sensitivity, and utilize LSRP (localized surface plasmon

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  • Label-free biochemical detection method reinforced by utilizing local surface plasma

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Embodiment 1

[0030] Embodiment 1 In this embodiment, glass is used as the substrate, and the diamond-shaped metal silver structure arranged in a quadrilateral is used as the detection chip; the non-labeled biochemical detection method enhanced by local surface plasmon of the present invention is used to test bacteria, and the specific reaction process adopted is Bind antibodies on the chip.

[0031] First, glass is used as the substrate to select the chip substrate, cleaned and treated for hydrophilicity; on the surface of the substrate, a diamond-shaped nano-silver structure with an area of ​​about 5 μm × 5 μm and a quadrilateral arrangement of 1 × 1 is produced by self-assembly of nanospheres. The characteristic size of the structure is 300nm; the nanostructure is metallized by silver plating to obtain a chip with the nanosilver structure on the surface of the substrate. Next, immobilize active groups on the surface of metal nanostructures (such as figure 2 shown in 3), and then throug...

Embodiment 2

[0033] In this embodiment, fused silica is used as the substrate, and the triangular metal gold structure arranged in hexagons is used as the detection chip; the local surface plasmon-enhanced unlabeled biochemical detection method of the present invention is used to test the toxin, and the specific reaction process adopted is Antigen binding on the chip.

[0034] First, fused silica is used as the substrate, cleaned and treated for hydrophilicity; on the surface of the substrate, a triangular nanostructure with a characteristic size of 50nm and hexagonal arrangement is produced by nanolithography; the nanostructure is metallized by gold plating to obtain A chip with a nano-gold structure on the surface of the substrate. Next, active groups are immobilized on the surface of the metal nanostructure, and then the antigen of the detected target bacterial molecule is immobilized on the active groups by a zero-distance coupling reagent. Through the above procedures, the chip activ...

Embodiment 3

[0036] In this embodiment, silicon is used as the substrate, and porous templates are produced by photolithography. The number of chip arrays is 150×150, and 150×150 protein antigens are integrated by the method of spotting, and the localized surface plasmon-enhanced Label-free biochemical detection methods test whether the analyte contains various types of proteins.

[0037]First, silicon is used as the substrate, cleaned and treated for hydrophilicity; nanostructure arrays with an array number of 150×150 are produced on the surface of the substrate by nanoimprinting, and the size of each subunit is 1000 μm×1000 μm; the nanostructure is metallized to obtain A chip with a nanometer metal structure on the surface of the substrate. The structure in each subunit can adopt different structures according to the different characteristics of the object to be tested. There are single-layer pentagrams, and there are upper and lower double-layer nano-silver structures composed of upper ...

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Abstract

A label-free biochemical detection method reinforced by utilizing local surface plasma is characterized in that the label-free biochemical detection method comprises the steps as follows: (1) preparing an LSPR detection chip according to a detected object; (2) selecting a specific biological molecule to activate the chip according to the detected object, and testing the spectrum reference value of the chip by a spectrum test system; (3) leading in a to-be-detected sample for detection, and obtaining a spectrum curve by test; and (4) judging whether the to-be-detected sample contains the detected object by analysing the spectral peak movement state of the spectrum, so as to quickly detect the detected object with high sensitivity and without a label, meanwhile, the array chip is used for realising high-efficiency and multi-channel detection. The method of the invention does not need complex equipment and does not use radioactive isotope, enzyme or fluorescence as a label, has the remarkable characteristics of low cost and high sensitivity, and can realise array chip. The method provides a simple and practical new method for fast detection of biochemical molecule.

Description

technical field [0001] The invention belongs to the field of biochemical detection and relates to a novel biochemical detection method, in particular to a high-sensitivity biochemical detection method utilizing localized surface plasmon resonance enhancement technology. Background technique [0002] Sensing technology is one of the three pillars of modern information technology and plays an important role in national security, scientific experiments, medical and health, and environmental monitoring. Biochemical sensing technology is an important branch of sensing technology, which is related to public safety, virus, bacteria detection, clinical medicine, environmental detection and other fields that are closely related to people's lives. Traditional biochemical sensing technologies usually require labels, are bulky, have low sensitivity (usually at the nanomolar level), cumbersome processes, and low efficiency. 90% of the workload is used for labeling, mainly using radioact...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/00G01N33/569G01N33/68
Inventor 邓启凌杜春雷罗先刚董小春杨欢
Owner INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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