Method for extracting antioxidant active substance in tasteless preserved soybean
An anti-oxidative activity, light tempeh technology, applied in cosmetics, antidote, food science and other directions, can solve the problems such as no research report on the extraction process of light tempeh antioxidant substances, and achieve the maintenance of safety, process flow and simple equipment. Effect
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[0016] The present invention is further described in detail through the following examples, but the technical content described in this example is illustrative rather than limiting, and should not be used to limit the protection scope of the present invention.
[0017] The steps of the method for extracting antioxidant active substances from light tempeh involved in the present invention are as follows:
[0018] (1) Pulverize the light fermented soya bean and pass through a 50-mesh sieve to form a light fermented soya bean coarse powder;
[0019] (2) Weigh 100 grams of light tempeh coarse powder, add 1500 mL of 75% ethanol solution to the light tempeh powder, extract at 75°C for 3 hours, repeat the extraction 3 times, combine the extracts and concentrate to 200 mL.
[0020] (3) After purification, the polyamide column is used for purification. The polyamide adopts 100-200 mesh and adopts a dynamic loading method; monitor the effluent with UV (ultraviolet chromatography) at 273...
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