Mosquito killing Bacillus sphaericus leafing agent and preparation method thereof

A Bacillus sphaericus and mosquito-killing technology, which is applied in the field of preparation of biological preparations, can solve the problems of high energy consumption, high cost, and large potency loss of Bacillus sphaericus, and achieve low potency loss rate and low transportation cost , the effect of small energy consumption

Inactive Publication Date: 2009-10-14
武汉天惠生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, adopting the spray drying method to produce mosquito-killing Bacillus sphaericus not only consumes a lot of energy and costs, but also loses a lot of potency, and the loss rate reaches more than 50%.

Method used

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  • Mosquito killing Bacillus sphaericus leafing agent and preparation method thereof
  • Mosquito killing Bacillus sphaericus leafing agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] After the mosquito-killing Bacillus sphaericus is successfully fermented, hydrochloric acid is added to adjust the pH to 5.0, and the slag is removed through a three-legged centrifuge (the screen mesh is 60 mesh, and the centrifuge speed is 1000 rpm). Take 75kg of fermentation filtrate, the power is 45w, the membrane area is 0.3m 2 , concentrated in an inorganic ceramic membrane module with a membrane pore size of 100nm, the operating temperature is 35°C, and the operating time is 6 hours and 25 minutes. After concentration, 66.3kg of clear liquid is discharged, and the bacteria slurry is released, and the bacteria remaining in the membrane and the pipeline are rinsed with a little water. Slurry, collect 8.6kg of bacterial slurry, and adjust the pH value to 4.8 with dilute hydrochloric acid simultaneously. Add the bacterium slurry to 0.67kg of dry corn cob particles with a particle size of 1-2mm in a weight ratio of 7.8%, fully mix the two in a tank mixer, and then diss...

Embodiment 2

[0035] After the mosquito-killing Bacillus sphaericus is successfully fermented, hydrochloric acid is added to adjust the pH to 5.0, and the slag is removed through a three-legged centrifuge (the screen mesh is 40 mesh, and the centrifuge speed is 1000 rpm). Take 75kg of fermentation filtrate, the power is 45w, the membrane area is 0.3m2 , concentrated in an inorganic ceramic membrane module with a membrane pore size of 100nm, the operating temperature is 36°C, and the operating time is 6 hours and 20 minutes. After concentration, 66.6kg of clear liquid is discharged, and the bacterial slurry is released, and the bacterial slurry remaining in the membrane and the pipeline is washed with a little water. , collect 8.5 kg of bacterial slurry, and adjust the pH value to 4.8 with dilute hydrochloric acid. Add 2.0124 kg of perlite particles to the bacterium slurry by weight ratio of 23.4%, fully mix the two in a tank mixer, then dissolve 68.8 g of coumarin in 1.4 L of clear water to ...

Embodiment 3

[0038] After the mosquito-killing Bacillus sphaericus is successfully fermented, hydrochloric acid is added to adjust the pH to 5.0, and the slag is removed through a three-legged centrifuge (the screen mesh is 60 mesh, and the centrifuge speed is 1000 rpm). Take 75kg of fermentation filtrate, the power is 45w, the membrane area is 0.3m 2 , concentrated in an inorganic ceramic membrane module with a membrane pore size of 100nm, the operating temperature is 37°C, and the operating time is 6 hours and 18 minutes. After concentration, 66.5kg of clear liquid is discharged, and the bacterial slurry is released, and the bacterial slurry remaining in the membrane and the pipeline is washed with a little water. , collect 8.6kg of bacterial slurry, and adjust the pH value to 4.8 with dilute hydrochloric acid. Add the bacterium slurry to 0.7432kg of dry corn cob particles with a particle size of 1-2mm at a weight ratio of 9.0%, fully mix the two in a tank mixer, and then dissolve 68.8g ...

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Abstract

The invention provides a mosquito killing Bacillus sphaericus leafing agent which is prepared by the following steps: firstly, concentrating mosquito killing Bacillus sphaericus ferment filtrate by a film concentration method; then, adsorbing the mosquito killing Bacillus sphaericus ferment filtrate by a porous carrier substance, mixing the mosquito killing Bacillus sphaericus ferment filtrate and sustained release agent and drying the mosquito killing Bacillus sphaericus ferment filtrate into a master batch at 40 DEG C-50DEG C; and finally, coating hydrophobing agent. The mosquito killing Bacillus sphaericus leafing agent is prepared by a low temperature production process, the loss rate (namely valence loss rate) of active components is only about 10 percent, and the solid recovery rate reaches about 95 percent. The technology ensures low valence loss rate, low energy consumption and low transportation cost in the production process. By measurement, the leafing agent has the effect on continuously killing Culex fatigans for more than 28 days (indoor measurement) by putting the 4mg / L leafing agent into water at one time.

Description

technical field [0001] The invention relates to a preparation method of a biological agent, in particular to a mosquito-killing Bacillus sphaericus (abbreviated as Bs) floating agent and a preparation method thereof. It belongs to the production process and formulation technology of microbial insecticides in biological pesticides. Background technique [0002] Bacillus sphaericus (Bs for short) is a beneficial microorganism that widely exists in the natural environment. A protein parasporium produced by its metabolism has a very high killing activity on mosquito larvae. Mosquitocidal Bacillus sphaericus and Bacillus thuringiensis israelia species (i.e. B.t.i) are identified by the World Health Organization (WHO) as two beneficial bacteria for biological control of mosquitoes. Several B.s strains are used in industrial production and production at home and abroad Mosquito control, such as the Bs2362 strain and C3-41 strain recommended by the World Health Organization to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P7/00
Inventor 林开春陈小龙李芒夏海洋肖凤平张用梅
Owner 武汉天惠生物工程有限公司
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