Production technique for extracting and separating isoflavone from red clover

A technology of red clover and production process, which is applied in the field of extraction and separation of isoflavones, can solve the problems of low market acceptance, heavy operation workload, cumbersome operation, etc., and achieve the effect of low cost, high yield and less equipment

Inactive Publication Date: 2009-10-21
花垣恒远植物生化有限责任公司
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Problems solved by technology

[0003] There are two main production methods for extracting and separating isoflavones from the existing red clover: one is to obtain a product with an isoflavone content higher than 20% after alcohol extraction, concentration, water precipitation, degreasing, drying and refining, and the operation is cumbersome , the...

Method used

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Embodiment Construction

[0007] 1. Grind the raw material of the dry part of the red clover grass to 1mm particles, weigh 500 kg / can of the raw material, and dynamically extract it with 95% methanol at 50°C for three times, and the extraction time is 2, 1, and 1 hour respectively. The medicinal liquid is filtered and concentrated until it has no alcohol smell. Use the mixed solvent extraction of the medium polar hydrophobic solvent methyl formate and the weak polar solvent n-hexane in a ratio of 1:1 until the active ingredient is completely transferred to the mixed solvent phase. Impurities have low solubility. The mixed solvent phase after extraction is recovered to 1 / 10 of the raw material in a concentrating pot, and n-hexane is added for crystallization for 24 hours, and the precipitate is vacuum-dried to obtain a 20% isoflavone product.

[0008] 2. Grind the raw material of the dry material on the red clover grass to 2mm particles, weigh 500 kg / can of the raw material, and dynamically extract it ...

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Abstract

The invention relates to a production technique for extracting and separating isoflavone from red clover, comprising the following steps: the dry red clover raw material is crushed and extracted by high degree alcohol in a dynamic way; after being filtered, the extracted liquid medicine is decompressed and concentrated to have no alcohol smell and then extracted by the mixed solvent formed by moderate polar hydrophobic solvent A and weak polar solvent B until active ingredients are completely transfered into mixed solvent phase; the extracted mixed solvent phase is concentrated and recovered, and then added with the weak polar solvent B for crystallization; the precipitate which is separated is processed by vacuum drying, so that the isoflavone product can be obtained. Compared with the prior art, the yield of the isoflavone prepared by the method is improved by 20-30%, and the cost of the production can be reduced by 20-30%.

Description

technical field [0001] The invention relates to a production process for extracting and separating isoflavones from red clover. Background technique [0002] Red clover (Trifolium pratense L) is a leguminous plant, and its aerial part contains a variety of isoflavones, mainly formononetin, biochanin A, daidzein, Genistein, Sission, and Ononin all have estrogen-like effects. It has been clinically proven that they can prevent breast cancer, prostate cancer, and colon cancer, and improve osteoporosis and menopause symptom. It is a promising natural health food. [0003] There are two main production methods for extracting and separating isoflavones from the existing red clover: one is to obtain a product with an isoflavone content higher than 20% after alcohol extraction, concentration, water precipitation, degreasing, drying and refining, and the operation is cumbersome , the operation workload is large and the cost is high; the other is a product with an isoflavone conten...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P35/00A61P19/10A61P15/00
Inventor 胡灿华
Owner 花垣恒远植物生化有限责任公司
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