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Method for producing xylo-oligosaccharide by utilizing cotton seed hulls

A technology of xylooligosaccharides and cottonseed hulls, which is applied in the field of xylooligosaccharides production, can solve the problems of no bifidobacteria value-added factor functionality, complex composition, and weak functionality, etc., to shorten the processing time and shorten the processing time , the effect of low production cost

Inactive Publication Date: 2009-10-28
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dilute acid hydrolysis products are oligosaccharides with a degree of polymerization of 2 to 15. The composition is very complex and the functionality is not strong, because the most functional xylooligosaccharides are xylobiose and xylotriose, xylotetraose to xyloheptose There is also functionality, xylo-oligosaccharides with a degree of polymerization greater than 7 no longer have the functionality of bifidobacteria growth factors

Method used

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  • Method for producing xylo-oligosaccharide by utilizing cotton seed hulls
  • Method for producing xylo-oligosaccharide by utilizing cotton seed hulls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: Weigh a certain amount of cottonseed hulls, soak in 0.2% (m / v) sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreat at room temperature for 24 hours, and rinse with water for 3 times, which can effectively remove the pigment substances in the raw materials .

[0029] Step 2: The wet cottonseed hulls are then treated with high-pressure steam at 180°C for 15 minutes.

[0030] Step 3: Add 1.0% (m / m) xylanase after cooling to 50°C with water (based on the dry weight of cottonseed hulls, xylanase is the commercial enzyme Novozymes Pentopan Mono BG), at a temperature of 45-50°C Under the condition of pH 4.0-pH 5.5, the enzyme was hydrolyzed for 36 hours under the condition of slow stirring or shaking.

[0031] Step 4: After the enzymolysis solution is boiled for 10 minutes to kill the enzyme, it is then filtered (filtered by a plate and frame filter) and vacuum concentrated (three-effect or four-effect vacuum concentration) to a sugar concentration o...

Embodiment 2

[0033] Step 1: Weigh a certain amount of cottonseed hulls, soak in 0.5% (m / v) sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreat at room temperature for 20 hours, and rinse with water for 5 times, which can effectively remove the pigment substances in the raw materials .

[0034] Step 2: The wet cottonseed hulls are then treated with high-pressure steam at 190°C for 7 minutes.

[0035] Step 3: Add 2.5% (m / m) xylanase after cooling to 50°C with water (based on the dry weight of cottonseed hulls, xylanase is the commercial enzyme Novozymes Pentopan Mono BG), at a temperature of 45-50°C And under the condition of pH4.0-pH5.5, after 24 hours of enzymatic hydrolysis under the condition of slow stirring or shaking.

[0036] Step 4: Boil the enzymatic solution for 10 minutes to kill enzymes, then filter and vacuum concentrate to a sugar concentration above 75% (m / m) to obtain xylooligosaccharide syrup.

Embodiment 3

[0038] Step 1: Weigh a certain amount of cottonseed hulls, soak in 0.3% (m / v) sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreat at room temperature for 24 hours, and rinse with water for 3 times, which can effectively remove the pigment substances in the raw materials .

[0039] Step 2: The wet cottonseed hulls are then treated with high-pressure steam at 185°C for 10 minutes.

[0040] Step 3: Add 1.0% (m / m) xylanase after cooling to 50°C with water (based on the dry weight of cottonseed hulls, xylanase is the commercial enzyme Novozymes Pentopan Mono BG), at a temperature of 45-50°C And under the condition of pH4.0-pH5.5, after 36 hours of enzymatic hydrolysis under the condition of slow stirring or shaking.

[0041] Step 4: Boil the enzymatic solution for 10 minutes to kill enzymes, then filter and vacuum concentrate to a sugar concentration above 75% (m / m) to obtain xylooligosaccharide syrup.

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Abstract

The invention discloses a method for producing xylo-oligosaccharide by utilizing cotton seed hulls, which adopts the cotton seed hulls as raw materials and comprises the steps: the cotton seed hulls are dipped in a dilute sulphuric acid solution according to a solid-to-liquid ratio of 1 to 10, pretreated at room temperature for 20-24 hours, treated in high pressure steam at temperature of 180-190 DEG C for 7-15 minutes, cooled down by water to 50 DEG C and then added with 1.0-2.5% xylanase for enzymolysis for 24-36 hours in slow stirring or vibration manner, after being boiled for 10 minutes to remove enzyme, enzymatic hydrolyzate is filtered and concentrated in a vacuum manner till sugar concentration is more than 75 percent to obtain the xylo-oligosaccharide syrup. The method is characterized in that the cotton seed hulls with more xylose content of xylan is adopted as raw materials; pretreatment is conducted to effectively remove coloring matters; high pressure processing can greatly shorten time; the technological process of xylo-oligosaccharide production is simple and practical, with relatively low production cost; and the production technique and method of xylo-oligosaccharide have good industrialized application prospect.

Description

technical field [0001] The invention relates to a production method of xylooligosaccharides, in particular to a method for producing xylooligosaccharides using cottonseed hulls as raw materials. Background technique [0002] my country is a big cotton producing country. In 2007, the country's cotton planting area was 82.65 million mu, and the cottonseed output was about 12 million tons, ranking first in the world. Based on this, it is estimated that the annual output of cottonseed hulls in my country is about 5.4 million tons. From the perspective of the utilization of cottonseed hulls in my country, except for a small amount of them used for the cultivation of edible fungi and the production of boards, a large amount of cottonseed hulls are discarded, and their biomass utilization rate is extremely low. The content of xylan in cottonseed hull is about 28%, which is abundant. Compared with other xylan-rich raw materials such as corncobs, cottonseed hulls have the characte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 丁长河洪丰陈复生李里特王罗琳
Owner HENAN UNIVERSITY OF TECHNOLOGY
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