Aspergillus fumigatus and application thereof

Abstract

The invention discloses an aspergillus fumigatus which can produce podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside, and application thereof. The aspergillus fumigatus TEQA CGMCC No. 3115 has the function of producing podophyllotoxin, epipodophyllotoxin biglucoside and flavonoids astragaloside which have the physiological property the same as that of Chinese podophyllum root. Theaspergillus fumigatus can be used for preparing podophyllotoxin and astragaloside. The method for preparing podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside has the advantages of simple fermentation conditions, easy bacterium culture and short production period, with potential application for industrialized mass production. Besides, the aspergillus fumigatus provides a new way forresource development of podophyllotoxin and has important meaning to protect ecological diversity of plants.

Description

A strain of Aspergillus fumigatus and its application technical field The invention relates to a strain of Aspergillus fumigatus and its application. Background technique Podophyllotoxin (podophyllotoxin) mainly exists in the Berberidaceae Podophyllum and its related plants. It is a class of natural compounds with a wide range of biological activities, including estrogen-like, anti-estrogen-like, anti-cancer, anti-viral , antifungal, sedative, inhibiting plant growth and antioxidant effects. In particular, it has strong anticancer activity, and its mechanism of action is to inhibit the depolymerization of tubulin to prevent cell division and inhibit the activity of DNA topoisomerase. The glycoside derivatives of podophyllotoxin, etoposide and teniposide, have low toxicity and have good curative effects on various tumor diseases such as small cell lung cancer and lymphoma, so they have become broad-spectrum anti-tumor drugs for clinical application. At present, podophyll...

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Problems solved by technology

Because the plant growth environment is complex, it is difficult to simulate, and its growth cycle is long, it is difficult to carry out large-scale artificial cultivation, and tissue culture technology is not suitable for podophyllum plants; in addition, the chemical structure of podophyllotoxins is complex, and chemical synthesis Although it can be carried out, the cost is too high, and the construction of engineered bacteria by means of genetic engineering cannot be carried out due to the large number of enzymes involved in the formation of such substances.

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