Method for confirming oxygen consuming content and oxygen consumption rate of oil pool microorganisms

A technology of oxygen consumption rate and microorganisms, which is applied in the fields of biochemical equipment and methods, determination/inspection of microorganisms, measuring devices, etc., to achieve the effect of rapid analysis and wide application range

Active Publication Date: 2009-11-11
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no reliable experimental method to determine that air injection para

Method used

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  • Method for confirming oxygen consuming content and oxygen consumption rate of oil pool microorganisms
  • Method for confirming oxygen consuming content and oxygen consumption rate of oil pool microorganisms
  • Method for confirming oxygen consuming content and oxygen consumption rate of oil pool microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In order to evaluate the oxygen consumption rate of the starch-based system and the N / P system (ammonium salt) in the process of activating the original microorganisms in the oil reservoir, this method is used to evaluate the oxygen consumption rate of the microorganisms in the oil reservoir. Under simulated reservoir temperature and pressure conditions (50°C and 10MPa), add water and air (gas-liquid ratio 4:1) from oil wells containing 5% by weight of nutrients to the culture container, and after 20 days of culture, take the gas Its components were quantified by gas chromatography to judge the utilization of oxygen in the process of microbial consumption of nutrients.

[0029] image 3 is the effect of N / P nutrients on O in the sampled gas during different periods of oxygen consumption 2 and CO 2 The change curve of composition ratio. It can be seen from the figure that under the condition of oxygen supplementation, the oxygen consumption of the original microorgani...

Embodiment 2

[0033] In order to study the inhibitory effect of nitrate on sulfate-reducing bacteria and optimize the nutritional system of microbial oil displacement, the method of the present invention is used to inject two nutritional systems into the culture container respectively for 30 days of cultivation. These two culture solutions are composed of: (1) 400ml of formation water, 2.0% of nutrient, cultivated in airtight anaerobic condition; (2) 400ml of formation water, 2.0% of nutrient, 0.5% of sodium nitrate, cultivated in airtight anaerobic condition. The above cultivations were carried out at a high temperature of 50°C.

[0034] During the cultivation process, regular samples were taken to analyze the concentration changes of nitrate-reducing bacteria and sulfate-reducing bacteria. Figure 5 .

[0035] Figure 5It is the change of sulfate-reducing bacteria concentration in formation water during cultivation at 50°C under the condition of presence and absence of nitrate after add...

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Abstract

The invention relates to a method for confirming oxygen consuming content and oxygen consumption rate of oil pool microorganisms, comprising the following steps: (1) injecting oil well produced water or oil well injected water containing 0.5-5 percent of nutrient components into a culture vessel by a displacement pump; (2) supplementing the culture vessel with oxygen or air, wherein the volume of the air flow is 1-5 times of that of the water sample; (3) culturing the water sample by simulating the oil pool temperature of 45-80 DEG C and the pressure of 10-20MPa for 5-30 days; (4) taking the water sample for analyzing density counting and community structure of flora; and (5) taking air sample for gas chromatography and calculating the consuming content of the oxygen by combing with the flora counting and analyzing result. The method can confirm the amount of oxygen needed by oil pool microorganisms for effective growth and propagation in different conditions to further calculate the total air needed to be injected in the oil pool, can provide effective research means for the optimization of nutrient systems and comments on origin microorganism oil-displacing effect and also can provide a basis for the design of on-the-spot injection proposals.

Description

technical field [0001] The invention relates to a method for determining oxygen consumption and oxygen consumption rate of microorganisms in oil reservoir environment by using high temperature and high pressure culture and gas chromatography analysis. Background technique [0002] Microbial flooding is an enhanced oil recovery technology with the characteristics of low cost, environmental friendliness and convenient construction. The original microbial flooding technology is to inject nutrients into the reservoir to stimulate the growth and metabolism of microorganisms in the reservoir itself, and their metabolites and bacteria will have complex physical, chemical and chemical interactions with formation rocks, oil and water. Biological reaction, thereby changing some physical and chemical properties of rock, oil and water, and finally improving the flow performance of crude oil to enhance oil recovery. In the oil reservoir environment, according to the different requiremen...

Claims

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Application Information

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IPC IPC(8): C12Q1/06G01N30/02
Inventor 程海鹰马先平冯庆贤倪方天梁建春柳敏滕克孟
Owner PETROCHINA CO LTD
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