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Method for detecting Al in drinking water or cells based on functionalized gold nanoprobe colour comparison

A functional and drinking water technology, applied in the direction of biological testing, material inspection products, test sample preparation, etc., can solve the problems of detectors with less Al3+, etc., and achieve simple and intuitive detection methods, simple and intuitive detection, and color rendering stable effect

Inactive Publication Date: 2009-11-11
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many ion detectors have been developed, but few have been applied to Al 3+ detector
So far, no functionalized AuNP-based detection of Al in water or in cells has been reported. 3+ report

Method used

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  • Method for detecting Al in drinking water or cells based on functionalized gold nanoprobe colour comparison
  • Method for detecting Al in drinking water or cells based on functionalized gold nanoprobe colour comparison
  • Method for detecting Al in drinking water or cells based on functionalized gold nanoprobe colour comparison

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 provides a method to detect Al in water system based on functionalized nanoparticles 3+ Methods. First, according to the Frens-Turkevich method, sodium citrate was used to reduce chloroauric acid to prepare gold particles with a particle size of 13 nm: the pentapeptide CALNN with a concentration of 1 mg / ml was added to the aqueous solution of gold particles with a particle size of 13 nm to make the pentapeptide CALNN The final concentration in the above mixed solution is 0.1mg / ml, let it stand at room temperature for 1 hour, centrifuge at 13000rpm and discard the supernatant; add the centrifuged precipitate to deionized water, then centrifuge at 13000rpm and discard the supernatant; Finally, the centrifuged precipitate was dissolved in deionized water to obtain a functionalized gold nanoparticle probe solution;

[0034] Add 20 μl, 12nmol / l functionalized gold nanoparticle probe solution to 180 μl of 16 kinds of metal ions of different concentrations: Na +...

Embodiment 2

[0037] Example 2 The synthesis method of p-GNPs is the same as Example 1. First, according to the Frens-Turkevich method, sodium citrate was used to reduce chloroauric acid to prepare gold particles with a particle size of 13 nm: the pentapeptide CALNN with a concentration of 1 mg / ml was added to the aqueous solution of gold particles with a particle size of 13 nm to make the pentapeptide CALNN The final concentration in the above mixed solution is 0.1mg / ml, let it stand at room temperature for 1 hour, centrifuge at 13000rpm and discard the supernatant; add the centrifuged precipitate to deionized water, then centrifuge at 13000rpm and discard the supernatant; Finally, the centrifuged precipitate was dissolved in deionized water to obtain a functionalized gold nanoparticle probe solution;

[0038] Acidify a drinking water sample to pH 3 with 6mol / l HCl to convert Al in drinking water to Al 3+form; then adjust the pH to neutral with 6mol / l NaOH. Add 20 μl of 12 nmol / l gold na...

Embodiment 3

[0039] Example 3 provides a method for detecting Al in cells based on functionalized nanoparticles 3+ Methods

[0040] First, according to the Frens-Turkevich method, sodium citrate was used to reduce chloroauric acid to prepare gold particles with a particle size of 13 nm: the pentapeptide CALNN with a concentration of 1 mg / ml was added to the aqueous solution of gold particles with a particle size of 13 nm to make the pentapeptide CALNN The final concentration in the above mixed solution is 0.1mg / ml, let it stand at room temperature for 1 hour, centrifuge at 13000rpm and discard the supernatant; add the centrifuged precipitate to deionized water, then centrifuge at 13000rpm and discard the supernatant; Finally, the centrifuged precipitate was dissolved in deionized water to obtain a functionalized gold nanoparticle probe solution;

[0041] First, the cervical cancer (Hela) cells were placed in the cell culture medium (DMEM) at a volume ratio of 5% CO 2 and 95% air cell cul...

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Abstract

The invention relates to a method for detecting Al in drinking water or cells based on functionalized gold nanoprobe colour comparison. The method uses functionalized gold nano-particles modified by polypeptide as a detection probe and detects Al in drinking water or cells with high sensitivity and selectivity. The detection limit of the drinking water is 0.1mumol / l and the linear range for detecting Al in cells is (1-10) mumol / l. The detection method is simple and quick, high in sensitivity and high in selectivity and can realize open hole detection.

Description

technical field [0001] The invention relates to the colorimetric detection of Al in drinking water or cells based on functionalized gold nanoparticles 3+ Methods. technical background [0002] The distance-dependent optical properties and large specific surface area of ​​metal nanoparticles make them useful as important colorimetric materials. The molar extinction coefficient of their surface plasmon resonance absorption is more than 100 times that of organic dyes. Taking advantage of their size- and shape-dependent optical properties, nanoparticle-based molecular recognition methods offer sensitivity and selectivity comparable to fluorescence methods (Chemical Reviews, Chem. Rev., 2005, 105, 1547-1562; Chemical Reviews, Chem. Rev., 2004, 104, 293-346). At present, sensors based on metal nanoparticles have been applied in many aspects such as DNA, protein, antigen-antibody immune reaction and metal ion detection (Journal of American Chemical Society, J.Am.Chem.Soc., 2003,...

Claims

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Application Information

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IPC IPC(8): G01N31/22G01N21/85G01N1/28G01N33/52B22F9/24
Inventor 王振新李晓坤孙琳琳王金娥
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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