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Polypeptide for regulating activity of FGFR3 and screening method and application thereof

An active, amino acid technology, applied in the field of peptides, can solve the problems such as unseen FGF, and achieve the effect of simple preparation, controllable quality and good application value

Inactive Publication Date: 2011-10-05
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although 23 FGFs have been discovered so far, there is no FGF that only specifically binds to FGFR3

Method used

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  • Polypeptide for regulating activity of FGFR3 and screening method and application thereof
  • Polypeptide for regulating activity of FGFR3 and screening method and application thereof
  • Polypeptide for regulating activity of FGFR3 and screening method and application thereof

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Embodiment Construction

[0021] The invention adopts phage display technology to screen the polypeptide regulating FGFR3 activity. Phage display technology is to insert the gene sequence of foreign protein or polypeptide into the appropriate position of the phage coat protein structural gene, so that the foreign gene is expressed with the expression of the coat protein, and at the same time, the foreign protein or polypeptide is displayed with the reassembly of the phage Biotechnology to the surface of phages. Some foreign proteins or polypeptides expressed on the surface of phage can specifically bind to target molecules. After the target molecule is coated on a solid-phase carrier, the phage display peptide library is screened, and the phage that can specifically bind to the target molecule can be isolated from many phages, and the amino acid sequence of the foreign protein or polypeptide can be obtained by screening obtained by DNA sequencing of phages.

[0022] The screening of phage display pep...

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Abstract

The invention discloses a polypeptide for regulating activity of FGR3 and screening method and application thereof, the polypeptide is composed of an amino acid sequence shown as Val-Thr-Ser-Pro-Thr-Thr-Leu-Pro-Gln-Leu-Met-Ser. The screening method is that, repeated co-incubation of a bacteriophague 12 peptide library and standard plate of enzymes coated with FGFR3, bacteriophague capable of binding specifically with the FGFR3 are selected, amplifying the bacteriophague in vitro and sequencing, amino acid sequence of the exogenic polypeptide shown on the bacteriophague is deduced; synthesizingpolypeptides artificially; verifying combination function of the polypeptides and the FGFR3, testing regulation function of the polypeptide to activity of the FGFR3. The popypeptides can be used forpreparing medicine for preventing and treating related diseases of FGFR3 function.

Description

technical field [0001] The invention relates to a polypeptide, in particular to a polypeptide regulating the activity of FGFR3 (fibroblast growth factor receptor 3), and also relates to a screening method and application of the polypeptide. Background technique [0002] FGFR (fibroblast growth factor receptor) is a kind of transmembrane tyrosine kinase receptor with autophosphorylation activity, combined with its corresponding ligand FGF (fibroblast growth factor), in the process of tissue and organ development and damage repair play an important role in. [0003] Four kinds of FGFRs have been found so far, namely FGFR1, FGFR2, FGFR3 and FGFR4. Among them, FGFR3 is a negative regulatory molecule that mediates endochondral ossification and plays an important role in bone development. Previous studies have shown that FGFR3 gain-of-function gene mutations inhibit the proliferation and differentiation of chondrocytes in the epiphyseal growth plate, causing a variety of skeleta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08G01N33/566A61K38/10A61P19/02A61P19/10A61P19/08
Inventor 陈林余瑛黄炜杜晓兰戚华兵金旻苏楠赵玲杨京
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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