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Application of bifidobacterium lactis in preventing urinary system stones

A technology for urinary system stones and Bifidobacterium lactis, which can be applied to urinary system diseases, medical preparations containing active ingredients, and medical raw materials derived from bacteria, etc. It can solve the problems that Bifidobacterium lactis has not been reported.

Inactive Publication Date: 2009-12-23
上海交通大学医学院附属第三人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) It can only selectively stimulate the growth and reproduction of beneficial bacteria (Bifidobacteria, etc.) in the intestines or activate metabolic functions
However, there is no report on the application of Bifidobacterium lactis in the prevention of urinary calculi

Method used

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  • Application of bifidobacterium lactis in preventing urinary system stones
  • Application of bifidobacterium lactis in preventing urinary system stones
  • Application of bifidobacterium lactis in preventing urinary system stones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Experiments on the ability of 10 kinds of edible lactic acid bacteria to decompose oxalic acid in vitro and their reproductive ability in milk culture solution

[0022] 1. Materials

[0023] (1) Experimental equipment:

[0024] 1. Instrument: 5% CO 2 Incubator (Forma, Model 3111), Autoclave (Shanghai Boxun Industrial Co., Ltd.), Weighing Balance, Electric Furnace, SmartSpec TM Plus spectrophotometer.

[0025] 2. Helber bacterial counting plate (Shanghai Yikuo Instrument Co., Ltd.)

[0026] 3. Turbidimetric Measuring Instrument (DENSIMAT, Meliai Company)

[0027] 4. Utensils: Erlenmeyer flask, graduated cylinder, straw, test tube (20ml), pipette, dropper.

[0028] 5. Others: optical microscope, lens cleaning paper, absorbent paper.

[0029] (2) Experimental reagents:

[0030] 1. Lactobacillus acidophilus (L.acidophilu KLDS 1.8701s), Lactobacillus paracasei (L.paracasei KLDS 1.0201), Enterococcus faecium (Enterococcaceae faecium KLDS6.0302), Bifidobacterium lactis ...

Embodiment 2

[0100] Making yoghurt for prevention of urinary calculi with Bifidobacterium lactis and its test

[0101] 1. Materials

[0102] (1) Experimental equipment:

[0103] 1. Instruments: water bath, refrigerator, 5% CO 2 Incubator, weighing balance, autoclave, stirrer, electric furnace.

[0104] 2. Utensils: Erlenmeyer flasks, graduated cylinders, straws, test tubes, pipettes, droppers.

[0105] 3. Helber bacterial counting plate: provided by Shanghai Yikuo Instrument Co., Ltd.

[0106] 4. Others: optical microscope, lens cleaning paper, absorbent paper, 150-mesh gauze, etc.

[0107] (2) Experimental reagents:

[0108] Dried and frozen pure strains of Bifidobacterium lactis: (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University)

[0109] 1. Skim milk: (Guangming brand skim milk powder, bought in supermarkets)

[0110] 2. Soft white sugar: (purchased in supermarkets, in line with GB317 "white sugar")

[0111] 3. Phenolphthalein, NaOH, etc....

Embodiment 3

[0149] Production and testing of yogurt for prevention of urinary calculus with Bifidobacterium lactis and Lactobacillus acidophilus

[0150] One, the material is the same as the material in Example 2. Among them, the dry and frozen pure strain of Lactobacillus acidophilus: (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University)

[0151] Two, method With the method in embodiment 2. Wherein the revival of strains: in the test tubes containing Bifidobacterium lactis and Lactobacillus acidophilus, 2ml of Bifidobacterium lactis and 2ml of Lactobacillus acidophilus.

[0152] 3. Results

[0153] 1. Sensory quality of 4 kinds of yoghurts made with different inoculum amounts of starter: See Table 4 and figure 2 .

[0154] Table 4 Effect of starter inoculum amount on sensory quality of yoghurt

[0155]

[0156] **: P<0.01, 3% vs 2%

[0157] *: P<0.05, 4% vs 2%, 3%, 5%; 5% vs 2%

[0158] When the inoculum amount was 4%, the sensory score wa...

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Abstract

The invention relates to application of bifidobacterium lactis. The technical scheme comprises application of the bifidobacterium lactis in preventing urinary system stones, and application of a composition of the bifidobacterium lactis and edible fungi, prebiotics or acceptable carriers in preventing the urinary system stones. The applications have the advantages of exploring new medical use and developing a new application field for the bifidobacterium lactis. The bifidobacterium lactis is safe and nontoxic, has strong pharmacological action, foreshows excellent medicinal prospect, and provides a urinary stone prevention good product which is very suitable for patients to take for a long term for clinic.

Description

technical field [0001] The present invention relates to the application of bifidobacterium lactis, more specifically, the present invention relates to the application of bifidobacterium lactis in preventing urinary calculus. Background technique [0002] Urolithiasis is one of the common urinary system diseases that have threatened human health for a long time, among which calcium oxalate stones account for 70% to 80%, and have a high recurrence rate. Therefore, its prevention is particularly important. [0003] The effect of oxalic acid on the supersaturation of calcium oxalate in urine is 23 times greater than that of calcium. Therefore, hyperoxaluria is an important risk factor for calcium oxalate stone formation. A new study found that 50% to 80% of urinary oxalic acid comes from diet, and the amount of oxalic acid absorbed by the intestine is an important factor affecting the excretion of urinary oxalic acid. If the absorption of intestinal oxalic acid can be reduced,...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K36/06A61K36/064A61P13/04A61K31/7016A61K31/702A61K31/715A61K35/745
Inventor 张士青赵树田顾欣李建涛
Owner 上海交通大学医学院附属第三人民医院
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