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Hepatitis A virus antigen saliva fast detection test paper strip

A hepatitis A virus detection test strip technology, which is applied in measurement devices, resistance to vector-borne diseases, instruments, etc., can solve the problems of high detection cost and test strips not suitable for on-site detection, etc., and achieves high sensitivity and suitable for on-site detection. Detection and self-test, highly specific effect

Active Publication Date: 2009-12-30
杭州艾力康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a test strip for rapid detection of hepatitis A virus antigen saliva, the test strip can use saliva as a sample, which solves the problem that the existing test strip is not suitable for on-site detection and the detection cost is high

Method used

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  • Hepatitis A virus antigen saliva fast detection test paper strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of colloidal gold pads

[0022] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust to pH 8.0, add 0.75 mg of corresponding polyclonal antibody against hepatitis A virus antigen (Shanghai Lingchao Biotechnology Co., Ltd.) to 100 ml of colloidal gold solution, stir at room temperature for 1 hour, add bovine serum white that is 5% by weight Protein (BSA) was blocked for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 22cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0023] Coating of nitrocellulose membrane

[0024] Dissolve the second monoclonal antibody against hepatitis A virus antigen (Shangh...

Embodiment 2

[0029] Preparation of colloidal gold pads

[0030] Prepare a colloidal gold solution with a diameter of 35nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.2, add 1 mg of the corresponding first monoclonal antibody against hepatitis A virus antigen (Shanghai Lingchao Biotechnology Co., Ltd.) to the colloidal gold solution, stir at room temperature for 1 hour, add bovine serum white that is 5% by weight Protein (BSA) was blocked for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold diluent to 100ml, spread 50cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane, and dry it at 37°C for 30 minutes to make a colloidal gold pad.

[0031] Coating of nitrocellulose membrane

[0032] Dissolve the second monoclonal antibody against hepatitis A virus antigen...

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Abstract

The invention discloses a hepatitis A virus antigen saliva fast detection test paper strip which comprises a bottom plate that is sequentially stuck with a sample pad, an aurosol pad, a nitrocellulose membrane and a sample absorbing pad; wherein the aurosol pad is attached with polyclonal antibody or first monoclonal antibody of anti-hepatitis A virus antigen marked by aurosol; the nitrocellulosemembrane is coated with a detection line formed by second monoclonal antibody of the anti-hepatitis A virus antigen, and a quality control line formed by double anti-antibody resisting the polyclonalantibody or the first monoclonal antibody; the polyclonal antibody or the first monoclonal antibody is matched with the second monoclonal antibody in pairs. The test paper strip adopts aurosol labeling technique to detect the hepatitis A virus antigen ingredient in saliva, and can be used for judging whether the patient is infected by hepatitis B virus; furthermore, the test paper strip has simple operation, rapid reaction speed, high sensibility, strong specificity, being economical and practical and the like, so as to be suitable for field test and self-test.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a test strip for rapid detection of hepatitis A virus antigen in saliva. Background technique [0002] Hepatitis A (hepatitis A for short) is a kind of viral hepatitis caused by hepatitis A virus (HAV), which is mainly transmitted through the fecal-oral route. , Utensils and close contact with daily life enter the gastrointestinal tract and spread through the mouth. Hepatitis A virus has strong resistance to various external factors and can survive in the external environment for a long time. It can be transmitted through various contaminated items (hands, daily necessities, clothing, sheets, etc.), water and food, and can also be carried by flies. And spread. [0003] The source of hepatitis A infection is usually acute patients and subclinical infected patients. Patients are most contagious from the late incubation period to 10 days after onset. The fecal-oral...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/569G01N33/577G01N33/532G01N33/558
CPCY02A50/30
Inventor 郑隆泗李洪江
Owner 杭州艾力康医药科技有限公司
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