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Use of 1,2,3,4,6-penta-O-galloyl-b-D-glucose in preparation of anti-flu drugs

A technology of galloyl group and glucose, applied in the field of food or cosmetic preparation of anti-influenza virus drugs, can solve problems such as drug resistance and influenza virus mutation, and achieve the effect of strong antiviral activity

Inactive Publication Date: 2010-01-06
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are four types of anti-influenza drugs in clinical use: ion channel blockers and NA inhibitors. However, due to the wide application of existing drugs in clinical practice, influenza viruses have mutated, and these drugs have been affected. varying degrees of drug resistance

Method used

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  • Use of 1,2,3,4,6-penta-O-galloyl-b-D-glucose in preparation of anti-flu drugs
  • Use of 1,2,3,4,6-penta-O-galloyl-b-D-glucose in preparation of anti-flu drugs
  • Use of 1,2,3,4,6-penta-O-galloyl-b-D-glucose in preparation of anti-flu drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Detection of cytotoxicity and anti-influenza virus activity of PGG

[0038] (1) The cytotoxicity detection method of PGG is as follows: 3×10 4 MDCK cells / well were inoculated in a 96-well plate, and cultured in MEM medium containing 10% (v / v) fetal bovine serum (containing penicillin 100 U / ml and streptomycin 100 μg / ml) for 24 hours. , remove the medium by suction, add 200 μl of MEM medium (penicillin 100U) containing vitamins with a volume percentage concentration of 1% (v / v) containing serially diluted concentrations (0.39 μg / ml-25 μg / ml, 2-fold dilution) PGG / ml, streptomycin 100μg / ml), at 37°C, 5% CO 2 After culturing in the incubator for 72 hours, the WST-1 method was used for detection. Three replicate wells were set up for each experimental group, and the experiment was repeated four times.

[0039] WST-1 method: Add 10 μl of WST-1 solution to each well of the 96-well plate after 72 hours of culture, at 37 ° C, 5% CO 2 After culturing in the incuba...

Embodiment 2

[0044] Example 2: Inhibitory Effect of Different PGG Concentrations on Influenza Virus Replication in MDCK Cells

[0045] Experimental method: put 8×10 5 MDCK cells / well were inoculated in a 12-well plate, and cultured in MEM medium (penicillin 100 U / ml, streptomycin 100 μg / ml) containing 10% (v / v) fetal bovine serum by volume for 24 hours. After washing the cells twice with phosphate buffer (PBS(-)), add influenza virus A / WSN / 33 (H1N1 ) 200 μl (virus infection dose is MOI=0.001). at 37°C, 5% CO 2 After infection in the incubator for 1 hour, the virus infection solution was sucked and discarded, the cells were washed twice with phosphate buffer, and PGG containing different concentrations (1.56 μg / ml-12.5 μg / ml, 2-fold dilution) was added with a volume percentage concentration of 1% (v / v) vitamin MEM medium (containing 100 U / ml of penicillin and 100 μg / ml of streptomycin), continued culturing for 48 hours and recovered the culture supernatant. The virus shed in the culture...

Embodiment 3

[0049] Example 3: PGG inhibits the replication of influenza virus in MDCK cells

[0050] Experimental method: put 8×10 5 MDCK cells / well were inoculated in a 12-well plate, and cultured for 24 hours in MEM medium (containing penicillin 100 U / ml and streptomycin 100 μg / ml) containing fetal bovine serum (10% (v / v) by volume concentration); After washing the cells twice with PBS (-), add 200 μl of influenza virus A / WSN / 33 (H1N1) diluted with serum-free MEM medium (containing penicillin 100 U / ml, streptomycin 100 μg / ml) to each well (virus infection Doses are at MOI=0.001). at 37°C, 5% CO 2 After infection in the incubator for 1 hour, draw and discard the virus infection solution, wash the cells twice with PBS(-), add 2ml of PGG containing different concentrations (1.56μg / ml~12.5μg / ml, 2 times dilution) containing volume percentage concentration The MEM culture medium (containing penicillin 100U / ml, streptomycin 100 μg / ml) that is 1% vitamin; Experimental group (add containing ...

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Abstract

The invention provides a use of 1,2,3,4,6-penta-O-galloyl-b-D-glucose in the preparation of anti-flu drugs, foods or cosmetics. The 1,2,3,4,6-penta-O-galloyl-b-D-glucose not only has very strong anti-viral activity, but also has activity of inactivating virus, and the activity concentration is out of the range of cytotoxicity, so that the 1,2,3,4,6-penta-O-galloyl-b-D-glucose can directly inactivate the virus, inactivate the contacted virus, lead the virus to lose infection ability or play a therapeutic role to cells infected by the virus, be used for developing drugs, be applied in the food field, such as beverages and the like or the cosmetics field, and be also widely applied in health care products which can prevent and treat the virus.

Description

technical field [0001] The invention belongs to the field of medicines, and particularly relates to the use of 1,2,3,4,6-penta-O-galloyl-b-D-glucose as preparation of anti-influenza virus medicine, food or cosmetics. Background technique [0002] Influenza virus is the pathogen of the largest respiratory infectious disease infected by humans. Due to the strong and frequent antigen mutation ability of influenza virus, there are small-scale epidemics in various countries around the world every year, resulting in the death of infected children and the elderly with weak immunity. remains one of the greatest public health threats facing humanity. In recent years, the prevalence of highly pathogenic avian influenza H5N1 has not only brought a devastating blow to the chicken industry, but also can infect people in contact with a high fatality rate of more than 50%, which has sounded the alarm for us. Human-to-human transmission will pose a huge threat to human survival. While peo...

Claims

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Application Information

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IPC IPC(8): A61K31/7024A61P31/16A23L1/30A23L2/52A61K8/60
Inventor 北里海雄王一飞张颖君刘格钱垂文熊盛
Owner JINAN UNIVERSITY
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