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Method for extracting antiviral ingredients and barbadosnut phenol from barbadosnut branches and leaves and the application thereof

A technology of jatropha phenol and branches and leaves is applied in the application field of anti-influenza A3 virus and type I, II herpes simplex virus, and can solve problems such as drugs that have not seen jatropha branches and leaves.

Active Publication Date: 2010-01-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Judging from the above literature reports, although Jatropha curcas has a wide range of medicinal value, the reported medicinal value is limited to some folk customary usages, and no systematic pharmaceutical research has been carried out. Medicines developed from Jatropha curcas branches and leaves, and there is no research report on extracting phenolic chemical components from Jatropha curcas branches and leaves to resist influenza A type 3 virus and type I and II herpes simplex virus

Method used

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  • Method for extracting antiviral ingredients and barbadosnut phenol from barbadosnut branches and leaves and the application thereof
  • Method for extracting antiviral ingredients and barbadosnut phenol from barbadosnut branches and leaves and the application thereof
  • Method for extracting antiviral ingredients and barbadosnut phenol from barbadosnut branches and leaves and the application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] First dry and pulverize the branches and leaves of Jatropha curcas, then extract with distilled water 15 times the weight of the dried branches and leaves at a temperature of 70°C for 24 hours, repeat the extraction twice, filter, and combine the filtrates; put the water filtrate at a temperature of 50°C, Concentrate under reduced pressure under the condition of a pressure of 0.075 MPa to contain 2 g / ml of crude drug to obtain the antiviral component concentrate.

[0073] Add dilute hydrochloric acid to the concentrated solution of the antiviral component to adjust the pH to 4.0, and then use D-101 macroporous resin column chromatography to separate, that is, wash the resin with distilled water first, and then use ethanol solution with a volume concentration of 30-85%. Gradient elution, and collect the 45-65% ethanol eluate; the collected ethanol eluate was concentrated under reduced pressure at 30°C and a pressure of 0.095MPa for 1 hour, and then freeze-dried at -85°C f...

Embodiment 2

[0075] First dry and crush the branches and leaves of Jatropha curcas, then extract with deionized water 10 times the weight of the dried branches and leaves at a temperature of 75°C for 20 hours, and filter; Concentrate under reduced pressure until the crude drug is 2g / ml to obtain the antiviral component concentrate.

[0076] Add dilute hydrochloric acid to the antiviral component concentrated solution to adjust the pH to 4.5, and then use HPD-600 macroporous resin column chromatography to separate, that is, first wash the resin with deionized water, and then use ethanol with a volume concentration of 30-85% The solution is subjected to gradient elution, and the 45-65% ethanol eluate is collected; the collected ethanol eluate is concentrated under reduced pressure at 35°C and a pressure of 0.090MPa for 1.5 hours, and then freeze-dried at -84°C for 48h , to obtain the crude extract of jatrophol.

Embodiment 3

[0078] First dry and pulverize the branches and leaves of Jatropha curcas, then extract them with distilled water 18 times the weight of the dried branches and leaves at a temperature of 80°C for 18 hours, repeat the extraction 3 times, filter, and combine the filtrates; put the water filtrate at a temperature of 70°C, Concentrate under reduced pressure under the condition of a pressure of 0.090 MPa to contain 2 g / ml of crude drug to obtain the antiviral component concentrate.

[0079] Add dilute hydrochloric acid to the antiviral component concentrated solution to adjust the pH to 5.5, and then use HPD-450 macroporous resin column chromatography to separate, that is, first wash the resin with deionized water, and then use ethanol with a volume concentration of 30-85% The solution is subjected to gradient elution, and the 45-65% ethanol eluate is collected; the collected ethanol eluate is concentrated under reduced pressure at 45°C and a pressure of 0.090MPa for 2.0 hours, and ...

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Abstract

The invention discloses a method for extracting antiviral ingredients and barbadosnut phenol from barbadosnut branches and leaves, comprising the following steps: the barbadosnut branches and leaves are dried and smashed, and then are extracted with distilled water, or deionized water or ethanol with 30-85 percent of volume concentration, and the extraction is repeated for 1-3 times, and then the filtration is carried out, and the filtrates are mixed, and then the filtrates are decompressed and concentrated to obtain concentrated solution with antiviral ingredients; gradient elution separation is carried out to the obtained concentrated solution with a macroporous resin column chromatography way, and the ethanol eluate is collected; after being decompressed and concentrated, the collected ethanol eluate is frozen and dried to obtain barbadosnut phenol crude extracts at the temperature of less than or equal to below 80 DEG C; the barbadosnut phenol crude extracts are dissolved in methanol solution, and the gradient elution separation is carried out in a preparative liquid chromatograph with 30-70 percent of volume concentration, and the collected eluate is frozen and dried to obtain barbadosnut phenol at the temperature of less than or equal to below 80 DEG C. the invention also discloses the application of the antiviral ingredients and barbadosnut phenol which are extracted from the barbadosnut branches and leaves and are used as anti-flu A 3 type virus and I and II type herpes simplex virus drugs.

Description

technical field [0001] The invention belongs to the technical field of extracting antiviral components and jatrophol from Jatropha curcas, a plant of Euphorbiaceae, in particular to a method for extracting antiviral components and jatrophol from branches and leaves of Jatropha curcas and their use as Anti-influenza A type 3 virus (A3) and I, II type herpes simplex virus (HSV-I, HSV-II) application. Background technique [0002] Jatropha curcas (Jatropha curcas) is a plant belonging to the Euphorbiaceae family and is widely distributed in tropical and subtropical regions. In my country, Yunnan, Guangdong, Guangxi, Guizhou, Fujian, Sichuan and other provinces are rich in resources. Jatropha curcas is a traditional medicinal plant. According to the "Dictionary of Traditional Chinese Medicine": "cures bruises, swelling, fractures, wounds, skin itching, eczema, and acute gastroenteritis." "Color Atlas of Commonly Used Chinese Herbal Medicines" records: "clearing heat, relieving...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/47A61K31/122A61P31/22A61P31/16C07C49/747A61K127/00A61K135/00
Inventor 唐琳陈放颜钫雷蕾刘娟
Owner SICHUAN UNIV
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