Fluorescence measuring systems for application fiber coupler
A fiber optic coupler and fluorescence measurement technology, applied in the field of fluorescence measurement systems, can solve the problems of difficult debugging, complex design, processing, monitoring, etc., and achieve the effect of simple and cheap manufacturing process and price, convenient assembly and adjustment, and good spatial location
Inactive Publication Date: 2010-02-10
GUANGZHOU FENGHUA BIOENG
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[0003] The light transmission of this fluorescence measurement system is essentially a lens-mirror system, which is not only complicated in design and processing, but also difficult to assemble and adjust the coaxiality of the excitation light path and the fluorescence receiving light path; especially the key components - The dichroic mirror needs to reflect the excitation light and transmit the fluorescence. Not only the coating process is complicated, but also it is difficult to debug in the system
In addition, this fluorescence measurement system also fails to monitor the intensity of the excitation light. The energy fluctuation of the excitation light source is not automatically corrected by the feedback system, which affects the data stability of the measurement results.
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[0019] Specific embodiments of the present invention are intended to be illustrated by taking a fluorescence measuring instrument for quantitatively measuring the level of phenylalanine (Phe) in blood using a Y-type fiber coupler and an N-type fiber coupler as an example. Measuring the level of phenylalanine (Phe) in blood is based on the ninhydrin reaction of phenylalanine in blood samples at a suitable pH value and temperature to form a fluorescent complex, adding dipeptide L-leucine- L-alanine enhanced this response. Copper reagent was added to stabilize the fluorescent complex and increase the fluorescence intensity. The fluorescence wavelength that this fluorescent compound produces under the light excitation of wavelength 390nm is 485nm, and its intensity is directly proportional to the concentration of phenylalanine (Phe), therefore, can apply the fluorescence of application Y-type optical fiber coupler that the present invention proposes measuring system( Figure 1 S...
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The invention discloses two fluorescence measuring systems for application fiber coupler. One is a fluorescence measuring system for an application Y type fiber coupler. Compared with the prior lens-reflector type fluorescence measuring system, the fluorescence measuring system for the application Y type fiber coupler has the advantages of easy design, easy arrangement of space position and convenient assembly regulation. In addition, the making technology and the price of the Y type fiber coupler are simpler and cheaper than the making technology and the price of a dichroic mirror. The otheris a fluorescence measuring system for an application N type fiber coupler. Compared with the prior lens-reflector type fluorescence measuring system, the system has the advantages of easy design, easy arrangement of space position and convenient assembly regulation because the N type fiber coupler has the property for synchronously transmitting exciting light signals, fluorescence signals and three-way optical signals for monitoring light source intensity. The making technology and the price of the N type fiber coupler are simpler and cheaper than the making technology and the price of the dichroic mirror. In addition, the fluorescence measuring system also has the function for monitoring the intensity fluctuation of the exciting light. The two fluorescence measuring systems can be applied to a special fluorescence measurer for quantitively measuring phenyl alanine (Phe) level in blood samples.
Description
technical field [0001] The invention relates to a fluorescence measurement system, and also relates to a method for realizing the fluorescence measurement system by using an optical fiber coupler. Background technique [0002] Existing sample "top excitation, top receiving" fluorescence measurement optical system such as figure 1 As shown: the excitation light of this fluorescence measurement system is emitted by the light source 1, passes through the condenser lens 2, passes through the excitation filter 3, is reflected by the dichroic mirror 4, and then acts on the sample cell 6 through the objective lens 5; The fluorescence produced by the sample in the sample cell after being excited by light passes through the objective lens 5, passes through the dichroic mirror 4 and the fluorescence filter 7, and then acts on the photosensitive surface of the photoelectric receiving device 9 through the receiving light lens 8 , so as to realize photoelectric signal conversion and com...
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Login to View More IPC IPC(8): G01J3/443G02B6/26G01N21/64
Inventor 朱延彬马俊富田振
Owner GUANGZHOU FENGHUA BIOENG