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Standardized high-yield culture technique based on biological characteristics of asafetida mushroom

A kind of technology of asafoetida and biology, applied in the field of high-yield cultivation of edible fungi, can solve the problems of restricting the healthy development of the asafoetida industry, increasing the risk of the asafoetida mushroom, and high pollution rate.

Inactive Publication Date: 2010-02-17
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Asafoetida is a low-temperature type of edible fungus. Its artificial cultivation has very strict requirements on temperature, and its cultivation is difficult.
In addition, there are still some prominent practical problems in the current artificial cultivation of Ferulic mushrooms, such as long production cycle, high pollution rate in the bag making process, and serious pests and diseases in the fruiting process, which greatly increases the risk of mushroom farmers investing in planting Ferulic mushrooms, seriously Restricting the Healthy Development of Ferulae Ferulae Industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Optimization of culture parameters of Pleurotus ferulai mycelium

[0017] The optimization of temperature and light parameters adopts the plate method. Use a Φ10mm sterile puncher to perforate and cultivate 7-day outer marginal mycelium on the plate medium. Use a sterile inoculating spatula to inoculate the colony on the Φ90mm plate medium. The center is placed in a light constant temperature incubator for cultivation. The experiment has 8 temperatures (15℃, 20℃, 25℃, 27℃, 29℃, 31℃, 33℃, 35℃) and light (dark light, 300lx, 400lx, 500lx, 600lx, 700lx, 800lx, 900lx) Treatment, each treatment is set 5 times to observe the influence of different temperature and light on the growth of Pleurotus ferulai.

[0018] The optimization of nutrient factors and pH value adopts the fermentation method, scrapes a certain amount of new-born mycelium from the outer margin that has been cultured on a plate medium for 7 days with a sterile inoculating hook, and inoculates it in a 250...

Embodiment 2

[0024] Example 2. Optimization of management parameters of Pleurotus ferulai

[0025] For cottonseed hulls, bran, corn flour, KH 2 PO 4 Four nutritional factors for L 9 (3) 4 (See Table 1) Orthogonal screening. Other nutritional factors: 1.0% white granulated sugar, 0.4% gypsum powder fixed, the rest is sterile water, pH value is 8-10 before sterilization. Inoculate the liquid strains in polypropylene bags (17cm×33cm) with different solid medium formulations and a filling amount of 1000g / bag according to 10% of the inoculation amount, sterilize at 121℃ for 3h, and place them in a light incubator after inoculation. Incubate at 25°C for 45 to 50 days. Each treatment was repeated 5 times to observe the effects of different combinations on the yield and quality of Pleurotus ferulai.

[0026] Table 1 Experimental factors and levels

[0027]

[0028] For day and night temperature, air relative humidity, light, CO 2 Concentration of four fruiting management parameters for L 9 (3) 4 (See ...

Embodiment 3

[0032] Example 3. Standardized cultivation techniques based on the biological characteristics of Pleurotus ferulai

[0033] Based on the biological characteristics of Pleurotus ostreatus, the standardized cultivation techniques for applying liquid seed production in the factory production of mushroom bags include inclined cultivation of mother seeds, seed bottle cultivation of original seeds, fermentation tank cultivation of cultivated species, and mushroom cultivation of mushroom bags. Several stages of fruiting culture and harvesting and processing. After culturing in a seed bottle and a fermenter, the fermentation broth is inoculated into the fruiting bag culture material and cultured at a constant temperature under the optimal culture parameters of the mycelium. After the mycelium is physiologically mature, the bacteria are scratched and the fruiting water is injected to carry out fruiting. Cultivation, post-harvest processing.

[0034] (1) Slope culture: scrape a small amount...

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Abstract

The invention provides biological characteristics which significantly influence the growth development of mycelium of asafetida mushroom and the yield and the quality of a son solid of the asafetida mushroom, wherein the biological characteristics comprise temperature, nutrition, humidity, CO2 concentration, illumination, pH value and the like. The invention also provides a standardized culture technique based on the biological characteristics of the asafetida mushroom, which applies liquid seed production to bacteria bag factory production. The technique comprises the steps as follows: bevelculture of mother seeds, seed bottle culture of raw seeds, fermenting tin culture of planting seeds, spawn running culture in mushroom producing bags, fruiting culture and processing after picking. Optimally, the step of fruiting bag production also comprises the step of liquid seed production, wherein a culture medium of the liquid seeds comprises the components as follows: 1.0% of wheat flour, 0.5% of cane sugar, 0.2% of yeast ointment, 1.5% of bran (cooking juice), 0.2% of monopotassium phosphate, 0.15% of bitter salt and the balance of water. The technique is practically tested and verified by Sinkiang Ion Beam Bio-engineering Center (Ltd.) and the result shows that the technique is a high-yield culture technique suitable for the asafetida mushroom. The biologic conversion ratio of dual-tide mushroom picking ranges from 80% to 100%.

Description

Technical field [0001] The invention relates to a high-yield cultivation technology of edible fungi, in particular to a standardized high-yield cultivation technology based on the biological characteristics of the Pleurotus ferulae and applying liquid seed production in the factory production of mushroom bags. Background technique [0002] Pleurotus ferulae Lanzi (Pleurotus ferulae Lanzi), also known as Pleurotus ferulae, Pleurotus ferulae, Pleurotus ostreatus, is a kind of rare edible fungus with extremely high quality that occurs in the inland regions of Southern Europe, North Africa, and Central Asia in late spring and early summer. Wild Asafoetide mushrooms in China are only distributed on Asafoetide beaches in desert areas of Xinjiang (Mulei, Tuoli, Altay, Yili, Tacheng, etc.) because they grow on Asafoetide (Ferula.asafoetide). It is named after the root. Pleurotus ferulai is a kind of high-protein, low-fat, rich in fungal polysaccharides, dietary fiber, vitamins and a vari...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 陈恒雷万红贵张军吕杰吕长武
Owner XINJIANG UNIVERSITY
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