Method for producing Trichoderma viride by fermenting peanut shells and waste potato starch liquid

A potato starch, Trichoderma viride technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., to achieve the effect of low production cost

Inactive Publication Date: 2010-02-17
HENAN INST OF ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This patent uses the waste liquid produced in the production process of peanut shells and potato starch to produce Trichoderma viri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example 1: Preparation of Trichoderma viride biological pesticide

[0015] Seed medium preparation: Weigh 200g potatoes, chop them, boil them in 1L tap water for 30 minutes, filter to remove residues, add 20g glucose, and dilute the volume to 1L to obtain potato medium. Divide into 5 1L Erlenmeyer flasks, sterilize at 121℃ for 20mm.

[0016] Fermentation medium preparation: the potato starch waste liquid is adjusted to pH 6.0-7.0 with sodium hydroxide solution.

[0017] Sterilization of peanut shells: After mixing the peanut shells and potato starch waste liquid according to 1:1 (W / W), sterilize at 121°C for 20 minutes.

[0018] Liquid seed preparation: inoculate Trichoderma viride into the PDA liquid medium, at 27°C, shake flask 200r / min, shake culture on a constant temperature shaker for 24 hours to obtain liquid seeds.

[0019] Liquid fermentation: Put the above fermentation medium into a 10L fermentor, sterilize at 121°C for 15 minutes, inoculate liquid seeds at a 5% inoculu...

Example Embodiment

[0021] Example 2: Control effect on mung bean wilt

[0022] After preparation, the green Trichoderma inoculum stored for three months is fully mixed with the fine soil of the field where mung bean wilt has been planted for many years at a mass ratio of 1:20, and placed in a plastic flower pot (8crn). The control is Mix peanut shells with soil. Twenty days after sowing, the emergence rate and disease severity were counted, the relative control effect was calculated, the emergence situation and disease severity were counted, and the experiment was repeated 3 times. It was found that the incidence of mung bean wilt disease treated by Trichoderma viride was reduced by 70% compared with the control.

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Abstract

The invention belongs to the technical field of biological pesticides and relates to a production method of a microbial pesticide. Peanut shells and waste potato starch liquid are fermented to produceTrichoderma viride and prepare an anti-biological inoculant, microbes produced by the production method can effectively control a plurality of fungal diseases and bacterial diseases of plants, and the production method has the advantages of low production cost, simple operation, being capable of effectively utilizing the peanut shells and the waste potato starch liquid as resources, causing no harm and reducing environmental pollution.

Description

technical field [0001] The invention relates to resource utilization of waste liquid produced in the production process of peanut shells and potato starch. Specifically, the waste liquid and peanut shells in the potato processing process are used to produce Trichoderma viride, which belongs to the technical field of biological pesticides. technical background [0002] Trichoderma belongs to the subphylum Deuteromycotina, order Pyrosporium, and Pylopodiumaceae. It is currently recognized as a promising biocontrol fungus, which can prevent and control various diseases on various crops. Trichoderma has attracted much attention due to its abundant resources, long-lasting effect, unique mode of action, safety to non-target organisms, and non-polluting environment. At present, it has been found that Trichoderma has multiple biological control effects, as Chinese patent (authorized announcement number: CN 100340655C) discloses the method that can utilize Trichoderma viride strain L...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12N1/14C12R1/885
Inventor 张建云崔树军谷立坤廉有轩金维平
Owner HENAN INST OF ENG
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