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Method for producing Trichoderma viride by fermenting peanut shells and waste potato starch liquid

A potato starch, Trichoderma viride technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., to achieve the effect of low production cost

Inactive Publication Date: 2010-02-17
HENAN INST OF ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This patent uses the waste liquid produced in the production process of peanut shells and potato starch to produce Trichoderma viride, so that the waste liquid of peanut shells and potato starch can be recycled. This patent method has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Trichoderma viride biopesticide preparation

[0015] Preparation of seed medium: Weigh 200g of potatoes, chop them, boil in 1L of tap water for 30 minutes, filter to remove residue, add 20g of glucose, and dilute to 1L to obtain potato medium. Divide into five 1L Erlenmeyer flasks and sterilize at 121°C for 20mm.

[0016] Fermentation medium preparation: the potato starch waste liquid is adjusted to pH 6.0-7.0 with sodium hydroxide solution.

[0017] Sterilization of peanut shells: After mixing peanut shells and potato starch waste liquid at a ratio of 1:1 (W / W), sterilize at 121° C. for 20 minutes.

[0018] Preparation of liquid seeds: Inoculate Trichoderma viride into PDA liquid medium, shake the flask at 200 r / min at 27° C., shake the flask at a constant temperature for 24 hours, and obtain liquid seeds.

[0019] Liquid fermentation: put the above-mentioned fermentation medium into a 10L fermenter, sterilize at 121°C for 15 minutes, inoculate liquid s...

Embodiment 2

[0021] Embodiment 2: To mung bean damp-off control effect

[0022] The Trichoderma viride inoculum that was stored for three months after preparation was fully mixed with the fine soil of fields where mung beans had been planted for many years and mung bean blight was serious in proportion to the mass ratio of 1:20, and placed in plastic flowerpots (8crn), compared with Mix peanut shells with soil. The emergence rate and disease severity were counted 20 days after sowing, the relative control effect was calculated, and the emergence and disease severity were counted. The experiment was repeated 3 times. It was found that the incidence of blight of mung bean treated with Trichoderma viride was reduced by 70% compared with the control.

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Abstract

The invention belongs to the technical field of biological pesticides and relates to a production method of a microbial pesticide. Peanut shells and waste potato starch liquid are fermented to produceTrichoderma viride and prepare an anti-biological inoculant, microbes produced by the production method can effectively control a plurality of fungal diseases and bacterial diseases of plants, and the production method has the advantages of low production cost, simple operation, being capable of effectively utilizing the peanut shells and the waste potato starch liquid as resources, causing no harm and reducing environmental pollution.

Description

technical field [0001] The invention relates to resource utilization of waste liquid produced in the production process of peanut shells and potato starch. Specifically, the waste liquid and peanut shells in the potato processing process are used to produce Trichoderma viride, which belongs to the technical field of biological pesticides. technical background [0002] Trichoderma belongs to the subphylum Deuteromycotina, order Pyrosporium, and Pylopodiumaceae. It is currently recognized as a promising biocontrol fungus, which can prevent and control various diseases on various crops. Trichoderma has attracted much attention due to its abundant resources, long-lasting effect, unique mode of action, safety to non-target organisms, and non-polluting environment. At present, it has been found that Trichoderma has multiple biological control effects, as Chinese patent (authorized announcement number: CN 100340655C) discloses the method that can utilize Trichoderma viride strain L...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12N1/14C12R1/885
Inventor 张建云崔树军谷立坤廉有轩金维平
Owner HENAN INST OF ENG
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