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Artificial culture method of Kyushu cordyceps sinensis and culture medium thereof

A culture medium and the technology of Kyushu worm, which is applied in the field of pharmacognosy, can solve the problems of restricting the large-scale production of Kyushu Cordyceps, the long cultivation period of the raw material Bean hornworm, and the cumbersome operation process, so as to achieve good comprehensive benefits and ecological benefits, and save energy consumption , The effect of simple production process

Inactive Publication Date: 2012-10-17
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Cordyceps obtained by this method has high nutritional value and medicinal ingredients, due to the long cultivation cycle of the raw material Scutellaria beanus and the cumbersome operation process, its price is relatively high, and considering the safety of crops, Scutellaria beanus Moth larvae, commonly known as bean dan, are one of the main pests of soybeans, and large-scale cultivation will pose potential agricultural hazards
Therefore, the existing culture method of Cordyceps Kyushu limits the large-scale production of Cordyceps Kyushu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Strains: Kyushu Cordyceps strains come from the China Forestry Microbial Strains Preservation Management Center and are stored on the slant of PDA medium in test tubes.

[0047] 2. Strain activation:

[0048] Take 20g of sucrose, 5g of peptone, 5g of yeast extract, 0.5g of magnesium sulfate, 0.5g of potassium dihydrogen phosphate, 15g of agar powder, and the rest of water to prepare 1L of medium, with natural pH, and 200ml of medium in a 500ml Erlenmeyer flask. Sterilize at 121°C for 30 minutes, cool to 45°C, pour into a sterilized plate, take the plate and cool it to room temperature, inoculate a piece of Cordyceps Kyushu strain (the size of a soybean grain) stored on the slope of the PDA medium in the center of the plate, wrap Cover with parafilm and incubate at 20°C for 20 days;

[0049] 3. Vaccination:

[0050] Prepare induction medium: glycerol 5g, peptone 25g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.5g, agar 20g, water 949g, pH natural; steri...

Embodiment 2

[0054] 1. Strains: Kyushu Cordyceps strains come from the China Forestry Microbial Strains Preservation Management Center and are stored on the slant of PDA medium in test tubes.

[0055] 2. Strain activation:

[0056] Take 40g of sucrose, 10g of peptone, 10g of yeast extract, 1g of magnesium sulfate, 1g of potassium dihydrogen phosphate, 20g of agar powder, and the rest of water to prepare 1L of medium, with natural pH, and 200ml of medium in a 500ml Erlenmeyer flask. Sterilize at 121°C for 30 minutes, cool to 55°C, pour into a sterilized plate, take the plate and cool it to room temperature, inoculate a piece of Cordyceps Kyushu strain (the size of a soybean grain) stored on the slope of the PDA medium in the center of the plate, wrap Cover with parafilm and incubate at 30°C for 7 days;

[0057] 3. Vaccination:

[0058] Prepare induction medium: 200g glycerol, 15g peptone, 1g potassium dihydrogen phosphate, 0.3g magnesium sulfate, 15g agar, 768.7g water, natural pH; steril...

Embodiment 3

[0062] 1. Strains: Kyushu Cordyceps strains come from the China Forestry Microbial Strains Preservation Management Center and are stored on the slant of PDA medium in test tubes.

[0063] 2. Strain activation:

[0064] Take 30g of sucrose, 7g of peptone, 8g of yeast extract, 0.5g of magnesium sulfate, 0.8g of potassium dihydrogen phosphate, 17g of agar powder, and the rest of water to prepare 1L of medium, with natural pH, and 200ml of medium in a 500ml Erlenmeyer flask. Sterilize at 121°C for 30 minutes, cool to 50°C, pour into a sterilized plate, take the plate and cool it to room temperature, inoculate a piece of Cordyceps Kyushu strain (the size of a soybean grain) stored on the slope of the PDA medium in the center of the plate, wrap Cover with parafilm and incubate at 25°C for 14 days;

[0065] 3. Vaccination:

[0066] Prepare induction medium: 80g mannitol, 20g peptone, 0.6g potassium dihydrogen phosphate, 0.4g magnesium sulfate, 18g agar, 881g water, natural pH; ster...

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Abstract

The invention discloses an artificial culture method of Kyushu cordyceps sinensis and a culture medium thereof. The artificial culture method comprises the following steps: preserving a Kyushu cordyceps sinensis strain on the inclined surface of a PDA culture medium of a test tube; sterilizing and cooling a bottled activating culture medium, and inoculating the Kyushu cordyceps sinensis strain saved on the inclined surface of the PDA culture medium to culture for 7-20 days at a temperature of between 20 and 30 DEC; sterilizing and cooling an induced culture medium to the room temperature and inoculating the cultured Kyushu cordyceps sinensis strain under aseptic condition; putting inoculated Kyushu cordyceps into a thermotank for culturing by scattered illumination; and after picking up sub-sinensis, ventilating and drying at a temperature of between 40 and 60 DEG C to obtain the Kyushu cordyceps sinensis. The invention has simple operation, short culture period, high conversion rate,low cost and no pollution and wastes, continuous and volume production and correct materials selection; and the formula has scientificity, innovation, and low price and is suitable to be popularized.

Description

Technical field: [0001] The invention belongs to the technical field of pharmacognosy, and relates to a method and a culture medium for inducing the formation of the fruiting body of the Cordyceps Kyushu, and an inducer for inducing the differentiation of the fruiting bodies of the Cordyceps Kyushu under artificial culture conditions. Background technique: [0002] Kyushu Cordyceps (Cordyceps kyushuensis Kobayasi) is a kind of medicinal fungus, because it is often found in Mengshan Mountain, Shandong Province, it is also called Mengshan Cordyceps, which belongs to Eumycophyta, Ascomycetes, Ergot Fungi ( Clavicipitales), fungi of the family Clavicipitaceae. The host of Cordyceps Kyushu is the larvae of bean horn moth (Clanisbilineata Walker), and its anamorph is Skyushuensis Verficillium Kyushue. [0003] Research has found that Cordyceps Kyushu has the functions of relieving cough and reducing phlegm, nourishing and strengthening, anti-oxidation, anti-aging, and anti-tumor....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00A01G1/04
Inventor 程显好金鑫刘林德杜华东图力古尔
Owner LUDONG UNIVERSITY
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