Method for preparing chitosan into water-soluble chitosan oligosaccharide

Abstract

The invention relates to a method for preparing chitosan into water-soluble chitosan oligosaccharide, which takes the chitosan as raw material and comprises the following steps of: treating the chitosan by rinsing and drying technique, and then soaking in material liquid prepared by purified water and food grade hydrogen peroxide; carrying out microwave radiation degradation treatment, and formingthe mixed liquid of low molecular weight chitosan oligosaccharide which can be dissolved in the material liquid and chitosan part of which is not dissolved; after that, filtering and obtaining the residue of the chitosan and the chitosan oligosaccharide solution; and finally, adding the chitosan oligosaccharide solution into absolute alcohol for alcohol precipitation, and then carrying out suction filtration and drying, thus obtaining the finished product of the water-soluble chitosan oligosaccharide. By adopting the microwave radiation degradation treatment technology and exciting energy bya physical method, the higher molecular weight chitosan is degraded into the low molecular weight water-soluble chitosan oligosaccharide with stable structure, no reaction by-product is generated in the degradation process, and no contaminant is produced; furthermore, compared with the preparation method in the prior art, the method has the advantages of advanced technique, simple working procedures, little production facility investment, high purity of the product, stable quality and the like, thus opening up wide market application prospect for preparing the chitosan oligosaccharide by taking the chitosan as raw material.

Description

technical field [0001] The invention relates to the technical field of physical degradation, in particular to a method for preparing chitosan into water-soluble chitosan oligosaccharide. Background technique [0002] Chitosan oligosaccharide, also known as chitosan oligosaccharide, is a natural high-activity animal cellulose product made from chitosan obtained after deacetylation of chitin through high-tech degradation and separation methods. Due to its unique pharmacological effects and the resulting broad market application prospects, it has attracted increasing attention, and has even been praised as the "sixth vital element of the human body" and "soft gold". [0003] Chitin is a pure natural polymer compound that widely exists in crustaceans such as shrimps and crabs, insects, and algae. Its annual biosynthetic resources are as high as 10 billion tons, of which the production of marine organisms is about 10 It is the second largest inexhaustible natural biological reso...

AI Technical Summary

Problems solved by technology

The disadvantage of the chemical degradation method is that it is easy to produce reaction by-products, which will eventually affect the purity of the product, so the product quality is difficult to control and the stability is poor; the enzymatic hydrolysis method uses certain enzymes to specifically crack and change the molecular structure to achieve the purpose of degradation. Since no other reaction by-products are generated during the entire degradation process, it has become a more commonly used method at present. Its disadvantages are that the process is relatively complicated, the degradation efficiency is low, and the product cost is high. At present, it is only in the stage of laboratory research and small batch trial production. There are still a series of technical problems to be solved for industrial scale production

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