PCR detection method for quickly detecting large coptotermes formosunus

A detection method and rapid technology, applied in the field of PCR detection, can solve the problems of low reliability, low probability of winged adults, and poor accuracy, and achieve the effects of ensuring public health safety, maintaining ecological balance, and protecting production safety.
CN101665828BInactive Publication Date: 2012-07-18中华人民共和国珠海出入境检验检疫局
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
中华人民共和国珠海出入境检验检疫局
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a PCR detection method for quickly detecting large coptotermes formosunus, which comprises conventional PCR detection and real-time fluorescence PCR detection. The PCR detection method comprises the following steps: preparing a DNA template for a sample to be detected, designing a specific primer and / or a probe, establishing a PCR reaction system for PCR amplification, andobserving, recording and photographing on a gel imaging system. With the advantage of high speed, stability, reliability and sensitivity, the PCR detection method for quickly detecting large coptotermes formosunus is applied to the detection of different-grade samples of large coptotermes formosunus, and lays a foundation for China's coptotermes formosunus class in molecular classification and the rapid identification of sibling species. The result of study can be directly applied to the quarantine identification of large coptotermes formosunus in inward and outward plant quarantine, and has important significance to the production safety protection of agriculture and forestry, biological balance maintenance, public health safety insurance in China.
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Description

technical field

[0001] The invention relates to the field of molecular diagnosis of biological species, in particular to a PCR detection method for rapid detection of termites. Background technique

[0002] Termites belong to the order Isoptera of the order Insecta, and there are more than 2860 species in 7 families, 286 genera, and they are a class of worldwide important pests. There are more than 400 kinds of termites recorded in my country, and there are many kinds of termites with great harm, especially the genus Coptotermes Wasmann (1896). One of the most threatening termite species to spread and spread. This termite has strong adaptability, wide distribution, and rapid reproduction. It can not only harm a variety of trees, eat hard wood and even cellulose-containing wood processed products such as paper, cotton cloth, leather products, etc., but also harm the plastic protection of buried communication cables. Nesting and agroforestry crops. The danger of this termite...

Claims

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