Preparation method and composition of clerodendranthus spicatus extract
The technology of extract and composition is applied in the field of preparation method and composition of kidney tea extract, which can solve the problems of loss of beneficial components of kidney tea, and achieve the effect of improving anti-inflammatory
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Embodiment 1
[0024] 1. Cleaning and drying process: take the kidney tea raw material, remove the sundries, wash with water, and dry in the sun.
[0025] 2. Crushing process: crush the sun-dried kidney tea into 40-80 mesh particles.
[0026] 3. Pretreatment process: Add water and cellulosic mold to the crushed kidney tea granules, react at 30-35°C for 5 hours, then raise the temperature to 50-60°C, react for 2 hours, cool to room temperature, and then Ultrasonic extraction of 25,000 Hz ultrasonic waves for 20 minutes, filtering, and the weight ratio of kidney tea and water is 1:4, and the weight / volume (kg / L) ratio of kidney tea and composite cellulase is 1:0.02 .
[0027] The compound cellulosic mold is a mixture of Trichoderma sp CGMCC 3.3068 and Trichoderma koningii CGMCC 3.2774, and the volume ratio of the expansion medium is 1:0.5.
[0028] 4. The post-treatment process is: extract the filtrate with ether for 3 times, combine the ether extracts, and evaporate to dryness at 50°C to ob...
Embodiment 2
[0032] 1. Cleaning and drying process: take the kidney tea raw material, remove the sundries, wash with water, and dry in the sun.
[0033] 2. Crushing process: crush the sun-dried kidney tea into 100-200 mesh particles.
[0034] 3. Pretreatment process: add water and cellulosic mold to the crushed kidney tea granules, react at 35-40°C for 2 hours, then raise the temperature to 50-60°C, react for 2 hours, cool to room temperature, and then Ultrasonic extraction of 25,000 Hz ultrasonic waves for 20 minutes, filtering, and the weight ratio of kidney tea and water is 1:10, and the weight / volume (kg / L) ratio of kidney tea and composite cellulase is 1:0.1 .
[0035] The compound cellulosic mold is a mixture of Trichoderma sp CGMCC 3.3068 and Trichoderma koningii CGMCC 3.2774, and the volume ratio of the expansion medium is 1:0.8.
[0036] 4. The post-treatment process is as follows: extract the filtrate three times with ether, combine the ether extracts, and evaporate to dryness ...
Embodiment 3
[0042] Except that butyl acetate is increased in ethyl acetate, and the volume ratio of ethyl acetate and butyl acetate is 10:0.5, all the other are the same as in Example 3, and the obtained extract C yield is calculated as 1.9% based on kidney tea raw material.
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