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Method for extracting purified plantarenaloside from herba lysimachiae, product of plantarenaloside and application of method

A technology of psyllium ether and calculus, which is applied in the field of extraction and purification of psyllium glycosides

Inactive Publication Date: 2010-04-14
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technological method provided by the present invention solves the problem of extracting and purifying psyllium glucoside from calcaneus, and the obtained product has high purity, strong activity, obvious anti-inflammatory and analgesic activity and in vitro stone-dissolving effect, and can be used as a drug in drug testing. It can be used as a chemical standard or reference substance, and can also be used as a pharmaceutical raw material for preparations

Method used

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  • Method for extracting purified plantarenaloside from herba lysimachiae, product of plantarenaloside and application of method

Examples

Experimental program
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Effect test

Embodiment 1

[0077] Embodiment 1 of the present invention: Take 1 kg of calculus herbal medicine, pulverize it, add 10 times the weight of water to decoct 3 times, each time for 1 hour, filter, combine the filtrate, concentrate under reduced pressure at 60 ° C to an extract with a relative density of 1.15 , on AB-8 macroporous resin (2kg), washed with 1.5 times column volume, followed by elution with 4 times column volume 40% ethanol, collected eluent, reclaimed ethanol to obtain crude psyllid glycoside. The crude product was repeatedly subjected to silica gel (200-300 mesh) column chromatography, and the eluent was chloroform:methanol:water system (8:1:0.1), the solvent was recovered under reduced pressure, and the pure plantagoside glycoside was obtained. (Purity greater than 98%).

Embodiment 2

[0078] Example 2 of the present invention: take 1 kg of calculus herbal material, add 15 times the weight of water to decoct twice, each time for 2 hours, filter, combine the filtrate, concentrate under reduced pressure at 80 ° C to an extract with a relative density of 1.25, and add HPD-400 macroporous resin (3kg), was washed with 1 column volume of water, followed by elution with 3 column volumes of 70% ethanol, the eluate was collected, and the ethanol was recovered to obtain the crude plantagoside. The crude product was subjected to reverse-phase column chromatography repeatedly, using chloroform:methanol:water system (8:1:0.1) as the eluent, the solvent was recovered under reduced pressure, and spray-dried to obtain pure plantagoside (purity greater than 98%).

Embodiment 3

[0079] Example 3 of the present invention: take 1 kg of calculus herbal material, add 8 times the weight of water to decoct 4 times, each time for 0.5 hours, filter, combine the filtrate, concentrate under reduced pressure at 50 ° C to an extract with a relative density of 1.10, and add D101 macroporous resin (1.5 kg), washed with 2 times column volume with water, followed by elution with 6 times column volume of 80% ethanol, collected the eluate, recovered ethanol, and obtained crude plantagoside. The crude product was subjected to gel column chromatography repeatedly, using chloroform:methanol:water system (6:1.5:0.2) as the eluent, the solvent was recovered under reduced pressure, and freeze-dried to obtain pure plantagoside (purity greater than 98%).

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Abstract

The invention discloses a method for extracting purified plantarenaloside from herba lysimachiae, a product of plantarenaloside and application of the method. The method comprises the steps of extracting and filtering dried or fresh entire plants, overground parts or underground parts of herba lysimachiae by conventional technologies, concentrating the filter liquor into extractum through reducing pressure, adding macroporous resin into the extractum, using ethylalcohol to elute, collecting alcohol eluant, recovering the solvent to get crude plantarenaloside, repetitively implementing silica gel column chromatography, reversed phase chromatography or gel column chromatography for the crude plantarenaloside, and recovering and drying the solvent to get the purified plantarenaloside. The purified plantarenaloside prepared by the method has the advantages of high degree of purity and strong pharmacological activity, can be used as chemical standard product or reference product of herba lysimachiae or the extract of the herba lysimachiae in medicine detecting, and can be used as medicine material for preparation. The invention has the advantages of low cost, and is applied to large-scale production.

Description

Technical field: [0001] The invention relates to a method for extracting and purifying psyllium glycosides from the traditional Chinese medicinal herb Caulis, as well as its products and applications, which belong to the technical field of traditional Chinese medicines. Background technique: [0002] Incarvillea argute (Royle) Royle, also known as Maozicao, is a plant of the genus Incarvillea L in the genus Incarvillea. Yanshanhong, rock morning glory; perennial herb, up to 1.5m. The rhizomes are woody and strong, and they are born in hot and dry valleys and hillside bushes. The dry or fresh whole herb of Lithiasis is used for medicinal purposes, with bitter, slightly pungent and flat nature (Editorial Board of State Administration of Traditional Chinese Medicine, Chinese Materia Medica [M], Shanghai Science and Technology Press, 1997, (7): 425) . The folks of the Yi people call the stone grass "Wabuyou" and change it to "Hepatitis Grass". "Buyou" research, Chinese herba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08G01N33/15A61K31/7048A61P29/00A61P1/16
Inventor 杨小生马琳夏勇兵梁冰郝小江
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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