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Micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and method for preparing the system

A microfluidic chip and gene diagnosis technology, applied in biochemical equipment and methods, material analysis and material analysis through electromagnetic means, can solve the problems of large sample volume, long separation time, and longer PCR process, and achieve Easy to operate, high separation efficiency, good screening effect

Inactive Publication Date: 2010-04-14
宁波基内生物技术有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, the large amount of sample consumed by capillary electrophoresis makes the PCR process longer, and the long separation time makes the result analysis not fast, and the sensitivity is not high enough. It takes more than 30 minutes to analyze a sample
Moreover, the viscosity of the gel is very high, making it difficult to fill the gel
It is easy to cause air bubbles in the column during gel electrophoresis, which makes the life of the capillary shorter

Method used

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  • Micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and method for preparing the system
  • Micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and method for preparing the system
  • Micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and method for preparing the system

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Embodiment 2

[0043] MES-Tris (80mM MES, 40mM Tris) was used as the background electrolyte, pH6.12, and 3.5% hydroxypropyl cellulose (HPC) was used as the sieving medium to form a sieving system. The ΦX174-HaeIII DNA standard fragment was successfully separated using this screening system, and the two bands of 271bp and 281bP were well separated ( figure 2 ). At the same time, using this system, the microfluidic chip gel-free sieving electrophoresis separation was performed on the PCR amplification product of the disease pseudohypertrophic muscular dystrophy. image 3 with Figure 4 They are the electrophoresis separation of human pseudohypertrophic muscular dystrophy negative samples and positive samples by using the screening system respectively. from image 3 It can be seen that the content of exon 47 in normal people is less than that of exon 6 and exon 13, and from Figure 4 It can be seen from the positive sample detection that the content of exon 47 is more than the content of e...

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Abstract

The invention discloses a micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and a method for preparing the system, and relates to the micro-fluidic chip electrophoresis technique. The system takes water as solvent, takes MES-Tris as background electrolyte, takes hydroxypropyl cellulose, hydroxypropylmethylcellulose, glycol cellulose, polyvinyl alcohol or polyoxyethylene and two to five compounds as sieving media, and takes glucose, glycerol, mannitol, or chitosan and two to four compounds as additives. The method for preparation comprises the following steps: firstly, preparing the background electrolyte, secondly, adding the sieving media, and thirdly, adding additives. The micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and the method for preparing the system have convenient and safe use, high separation efficiency and excellent effect, and is suitable for separating PCR products of clinical diseases, thereby making reference for diagnosing diseases.

Description

technical field [0001] The invention relates to microfluidic chip electrophoresis technology, in particular to a microfluidic chip electrophoresis gel-free screening system for gene diagnosis and a preparation method thereof. Background technique [0002] Capillary electrophoresis has been able to separate proteins and DNA fragments. Gel is a commonly used electrophoresis medium in capillary electrophoresis. Due to the high viscosity of the gel, it is resistant to convection, can reduce the diffusion of separated substances, and can well limit the broadening of the band. The separation effect is good, the separation degree is high, and the column The efficiency is extremely high, and it is widely used in scientific research and medical disease diagnosis, especially in the detection of PCR products in genetic diagnosis and DNA sequence determination. However, the large amount of sample consumed by capillary electrophoresis makes the PCR process longer, and the long separatio...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/447
Inventor 翁毅刘威
Owner 宁波基内生物技术有限公司
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