Method for extracting chlorogenic acid

An extraction method, chlorogenic acid technology, applied in the field of chlorogenic acid extraction and purification technology, can solve the problems of unfavorable chlorogenic acid preservation, complicated post-processing, and difficulty in meeting optimal conditions, so as to shorten the production cycle and ensure extraction effect, the effect of saving energy consumption

Inactive Publication Date: 2010-05-12
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The temperature of the semi-bionic method is too high, which is likely to be unfavorable for the preservation of chlorogenic acid; the change of pH will inevitably require monitoring equipment, and the monitoring also has hysteresis, making it difficult for the reaction to meet the optimal conditions during industrial production; and a variety of solvents are used , which also complicates the post-processing

Method used

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  • Method for extracting chlorogenic acid

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Effect test

Embodiment 1

[0029] Get 10kg of fresh leaves of eucommia ulmoides (3.8% chlorogenic acid content), pressurize to 80MPa in the press, adjust the pH to 4 with sulfuric acid, pass through the NKA-II macroporous resin column with a column volume of 5L, and use 20L deionized water respectively Wash away impurities with 10L 18% ethanol, then elute chlorogenic acid with 20L 50% ethanol, collect the eluate, and finally remove macromolecular impurities through a hollow fiber ultrafiltration membrane with a molecular weight cut-off of 2000, and then use DK4040 type The nanofiltration membrane is concentrated, and the concentrated solution is spray-dried to finally obtain 352 g of chlorogenic acid finished product with a content of 90.5%.

Embodiment 2

[0031] Get 15kg of fresh leaves of Eucommia ulmoides (3.9% chlorogenic acid content), pressurize to 90MPa in the press, adjust the pH to 3.5 with phosphoric acid, pass through the NKA-9 macroporous resin column with a column volume of 5L, and use 25L deionized water and 10L of 20% ethanol was used to wash away impurities, and then 23L of 50% ethanol was used to elute chlorogenic acid, and the eluate was collected. Finally, the eluate was first passed through a hollow fiber ultrafiltration membrane with a molecular weight cut-off of 2000 to remove macromolecular impurities, and then the DL4040 nanometer filter was used to remove impurities. The filter membrane was concentrated, and the concentrated solution was spray-dried to finally obtain 545 g of finished chlorogenic acid with a content of 94.2%.

Embodiment 3

[0033] Get 20kg of fresh leaves of eucommia ulmoides (3.8% chlorogenic acid content), pressurize to 100MPa in an extruder, adjust the pH to 3 with hydrochloric acid, pass through the HZ-806 macroporous resin column with a column volume of 5L, and use 30L deionized water and 10L of 10% ethanol was used to wash away impurities, and then 25L of 50% ethanol was used to elute chlorogenic acid, and the eluate was collected. Finally, the eluate was first passed through a hollow fiber ultrafiltration membrane with a molecular weight cut-off of 2000 to remove macromolecular impurities, and then used JY-NF300 Concentrate with a type nanofiltration membrane, and the concentrated solution is spray-dried to finally obtain 673g of chlorogenic acid finished product with a content of 96.1%.

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Abstract

The invention relates to a method for separating and purifying chlorogenic acid, which is low in cost, large in yield, short in cycle and easy to industrialize. Process steps of the method include pressing, macroporous resin adsorption, membrane concentration and spray drying. The method adopted for producing chlorogenic acid has the advantages of simple process, little energy consumption and guarantee of extremely high purity.

Description

Technical field: [0001] The invention relates to an extraction and purification process of chlorogenic acid, in particular to a method for extracting chlorogenic acid from fresh Eucommia leaves. Background technique: [0002] Chlorogenic acid (Chlorogenic acid) system name is (1S, 3R, 4R, 5R)-3-[[3-(3,4-dihydroxyphenyl)-1-oxo-2-propenyl]oxy]- 1,4,5-Trihydroxycyclohexanecarboxylic acid, molecular formula C 16 h 18 o 9 , with a molecular weight of 354.30, is a depsipolic acid produced by caffeic acid (Caffeic acid) and quinic acid (Quinic acid, 1-hydroxyhexahydrogallic acid). A phenylpropanoid compound with the following structure: [0003] [0004] Chlorogenic acid becomes anhydrous compound at 1°C, melting point at 2°C, [α] 24D-3°C (c=2.8). The solubility in water at 2°C is 4%, and the solubility in hot water is even greater; it is easily soluble in ethanol and acetone, and very slightly soluble in ethyl acetate. Due to the instability of chlorogenic acid itself, hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56C07C67/48B01D61/58B01D15/08
Inventor 刘东锋张翼杨成东
Owner NANJING ZELANG MEDICAL TECH
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