New thymosin chitosan microsphere type oral medicinal preparation and preparation method thereof

A technology of chitosan microspheres and pharmaceutical preparations, which is applied in the direction of drug combinations, pharmaceutical formulas, and medical preparations of non-active ingredients. explanation, low-cost effect

Inactive Publication Date: 2010-06-02
铁岭鹿宝堂药业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the use of tablets will cause a lot of unnecessary waste
[0009] Although some progress has been made

Method used

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  • New thymosin chitosan microsphere type oral medicinal preparation and preparation method thereof
  • New thymosin chitosan microsphere type oral medicinal preparation and preparation method thereof
  • New thymosin chitosan microsphere type oral medicinal preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Extraction of pig thymosin by low pH value method

[0044] Raw material: pig thymus, source: commercially available

[0045] The preparation method is as follows:

[0046] 1) Remove the fat from the pig thymus and wash it with distilled water. Take 90 g of thymus and grind it with distilled water at a ratio of 1:3 (w / v, 90 g / 270 ml).

[0047] 2) Adjust the pH value to 3.5 with 4mol / L HCl.

[0048] 3) Repeated freezing and thawing at -20°C for 3 times, 24 hours each time.

[0049] 4) After thawing at room temperature, centrifuge at 7000rpm at 4°C for 30min, and keep the supernatant.

[0050] 5) Use 4mol / L NaOH to adjust the pH value to 6.8±0.2.

[0051] 6) Heat in a water bath at 80°C for 5 minutes, and immediately cool at -20°C for 12 hours.

[0052] 7) After melting, centrifuge at 7000rpm at 4°C for 30min, and keep the supernatant.

[0053] 8) Filter with four layers of filter paper and then filter with a 0.22 μm filter membrane to sterilize.

[0054] 9)...

Embodiment 2

[0057] Example 2 A new porcine thymosin chitosan microsphere oral pharmaceutical preparation

[0058] The preparation method is as follows:

[0059] 1) Weigh 1g of chitosan fine powder, dissolve it in 2% (w / v) acetic acid solution, dilute to 100ml, make a chitosan acetic acid solution with a concentration of 1% by mass to volume, and fully swell overnight .

[0060] 2) Take NaCl and 35 mg of porcine thymosin obtained according to the method of Example 1 according to Table 1, add it to 3.5 ml of chitosan acetic acid solution, stir until completely dissolved, and use it as the water phase.

[0061] Get 7ml oil phase according to table 1.

[0062] 3) Add the water phase to the oil phase drop by drop (the dropping speed is maintained at 1 drop / second), and stir with a magnetic stirrer at a certain stirring speed for 30 minutes to form a stable W / O emulsion.

[0063] 4) Add 25% glutaraldehyde dropwise to the W / O emulsion, stir with magnetic force, the stirring time is as shown i...

Embodiment 3

[0073] Embodiment 3, a kind of new porcine thymosin chitosan microsphere oral pharmaceutical preparation

[0074] The preparation method is as follows:

[0075] 1) Take by weighing 1g, 2g, 3g chitosan fine powder respectively, dissolve in the acetic acid solution of 2% (v / v), dilute to 100ml, make according to mass volume ratio, concentration is respectively 1%, 2%, 3% chitosan acetic acid solution, fully swell overnight.

[0076] 2) a certain amount of NaCl and a certain amount of pig thymosin obtained by the method of embodiment 1 are added in the 7ml chitosan acetic acid solution, NaCl and thymosin are 1: 1 by weight, the weight of chitosan and thymosin is such as table 2, Stir until completely dissolved, as the water phase.

[0077] Get the liquid paraffin containing 7% Span80 as the oil phase; the volume ratio of the oil phase and the water phase is shown in Table 2.

[0078] 3) The water phase was added dropwise to the oil phase (the dropping speed was maintained at 1...

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Abstract

The invention relates to a new thymosin chitosan microsphere type oral medicinal preparation and a preparation method thereof. The technical scheme is that the new thymosin chitosan microsphere type oil medicinal preparation is obtained by utilizing chitosan to microencapsulating thymosin. The preparation method comprises the following steps of: dissolving the chitosan in an acetic acid solution to form a chitosan and acetic acid solution; adding inorganic salt and the thymosin in the chitosan and acetic acid solution and stirring to fully dissolve the inorganic salt and the thymosin to be used as a water phase; using liquid paraffin containing an emulsifier as an oil phase; dropwise adding the water phase into the oil phase and stirring; dropwise adding a cross-linking agent, stirring, and then carrying out cross-linking solidification; centrifuging, washing and drying the obtained product. The thymosin chitosan microsphere type oil medicinal preparation realizes the slow medicament release in bodies with thymosin so as to provide foundation for the treatment of the clinical thymosin and auxiliary medicament administration.

Description

technical field [0001] The invention belongs to the field of food and pharmacy, and in particular relates to a novel thymosin chitosan microsphere oral pharmaceutical preparation and a preparation method thereof. Background technique [0002] Thymosin is a polypeptide hormone secreted by thymus tissue epithelial cells. In 1966, Golden extracted it from calf thymus, and since then named this biologically active substance thymosin. Its molecular weight is concentrated below 10,000 Da, and it is a complex of several polypeptides. Since thymosin has obvious immunomodulatory effects, tumor adjuvant therapy, and hepatitis adjuvant therapy in clinical applications, thymosin, whether extracted from animals or synthesized by molecular methods, has attracted more and more attention in the field of medicine and health care. . [0003] However, thymosin, as a small biologically active peptide, has a very short half-life (about 30s) in vivo, so the research on its dosage form is becom...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K38/22A61K47/36A61P37/02A61P35/00A61P1/16
Inventor 胡风庆战荣荣
Owner 铁岭鹿宝堂药业集团有限公司
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