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Application of carbon complementary device for cultivating microalgae in a large-scale way

A technology for large-scale cultivation and carbon supplementation, which can be used in microorganism-based methods, enzymology/microbiology devices, biochemical cleaning devices, etc., and can solve the problems of large power consumption and complex equipment for oxygen analysis.

Active Publication Date: 2010-06-09
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to overcome the problems of high oxygen desorption power consumption and complex equipment for directly adding carbon dioxide in the closed photobioreactor when microalgae are cultivated in a closed photobioreactor, and to provide a method for cultivating microalgae on a large scale. The new application of the carbon replenishment device in the closed culture of microalgae can effectively add carbon dioxide to the microalgae culture solution in the closed photobioreactor, and / or analyze the dissolved oxygen produced in the culture solution without causing oxygen Accumulation, i.e. carbon replenishment and / or deoxygenation

Method used

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  • Application of carbon complementary device for cultivating microalgae in a large-scale way
  • Application of carbon complementary device for cultivating microalgae in a large-scale way
  • Application of carbon complementary device for cultivating microalgae in a large-scale way

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Spirulina was cultured in a closed-type photobioreactor. The algal species were from the Institute of Process Engineering, Chinese Academy of Sciences, the species was Spirulina Platensis, the medium was Zarrouk medium, and the initial concentration of sodium bicarbonate in the culture medium was 0.1mol / L.

[0054] See figure 2 and image 3 . In high 100cm, wide 80cm, thickness is 20cm, and material is the well-type container 4 of plexiglass plate that is 2cm thick and is not lower than the liquid level in the container, and the vertical dividing plate 5 that has 20 centimeters gaps with the bottom of the container, The partition is a plastic plate with a thickness of 1.0 cm, located in the middle of the thickness direction of the well-type container, and the width matches the well-type container; 8 glass sand core gas distributors 6 of model G2 are installed at the bottom of the well-type container, and the gas The distributor is located directly below the bulkhead...

Embodiment 2

[0061] Spirulina was cultured in a closed-type photobioreactor. The algal species were from the Institute of Process Engineering, Chinese Academy of Sciences, the species was Spirulina Platensis, the medium was Zarrouk medium, and the initial concentration of sodium bicarbonate in the culture medium was 0.1mol / L.

[0062] See figure 2 and Figure 4 . In high 100cm, wide 40cm, thickness is 20cm, and material is the well-type container 4 of 2cm thick plexiglass plate and is equipped with not lower than the liquid level in the container, and the vertical dividing plate 5 that has 20 centimeters gaps with the bottom of the container, The partition is a plastic plate with a thickness of 1.0 cm, located in the middle of the thickness direction of the well-type container, and the width matches the well-type container; four glass sand core gas distributors 6 of model G2 are installed on the bottom of the well-type container, and the gas The distributor is located on the downstream...

Embodiment 3

[0069] Spirulina was cultivated in a tubular photobioreactor, and the plate photobioreactor in Example 2 was changed into a tubular photobioreactor.

[0070] The cultivation system is composed of 1 group of tubular photoreactors 12 and 2 trap-type carbon replenishing devices of Example 2. The two trap-type carbon replenishment devices are connected in parallel and connected with both ends of the tubular photoreactor group to form a loop, such as Figure 4 shown.

[0071] A group of tubular photoreactors consists of 40 tubular photoreactor units connected in parallel. Specifications of the tubular photobiological reaction unit: the inner diameter of the tube is 3cm, the outer diameter is 4cm, and the length is 25m. Every 10 tubes form a group side by side, and the two ends are connected by manifolds, a total of 4 groups. Tube material is glass. Four sets of tubular photobioreactors are connected in parallel. placed horizontally.

[0072] Others are with embodiment 2.

[...

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Abstract

The invention belongs to the field of microalgae cultivation and in particular relates to an application of a carbon complementary device for cultivating microalgae in a large-scale way by adopting closed microalgae cultivation; the application is that carbon dioxide is supplied in a high-efficiency way in the closed microalgae cultivation and / or dissolved oxygen generated in the nutrient solution is resolved; the carbon complementary device is a trap type carbon complementary device which is used for supplying carbon dioxide in the closed microalgae cultivation for carbon supplement and / or the dissolved oxygen generated in the nutrient solution is resolved. In the invention, carbon dioxide can be supplemented to the nutrient solution, so as to maintain the requirements of algae cells to a carbon source; in addition, dissolved oxygen in a photo reactor unit can be timely and effectively removed, so as to prevent overhigh dissolved oxygen concentration from inhibiting the growth of thealgae cells; the invention has high carbon dioxide absorption utilization rate, can effectively reduce the cultivation cost of the microalgae, well control the flowing rate of the nutrient solution, effectively reduce the power consumption of the fluid circulation and strengthen the mixing effect in the photo reactor unit.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to the use of a carbon replenishment device for large-scale cultivation of microalgae in the closed cultivation of microalgae. The dissolved oxygen generated in the liquid is analyzed. technical background [0002] Microalgae has high application value and has become an important source of materials in the fields of human food, medicine, dyes, and fine chemicals. With the depletion of fossil energy such as petroleum and coal, the biorefinery based on biomass has attracted people's attention. As an important renewable energy source, microalgae can provide biomass such as oil, starch, and cellulose, and has potential application value in the field of biorefinery. [0003] The culture methods of microalgae can be divided into closed type and open type. The open type refers to the use of open pool culture devices, such as track pools and circular shallow pools. It has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/04C12N1/12C12R1/89
CPCC12M21/02C12M41/34C12M41/32
Inventor 丛威苏贞峰薛升长杨成砚
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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