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Bacterial agitation leaching experimental facility and method for measuring oxygen mass transfer coefficients

An experimental device, stirring and leaching technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as inability to carry out online control and measurement, and achieve stability and operability performance, avoid water evaporation loss, and reduce the effect of operating steps

Inactive Publication Date: 2010-06-16
GENERAL RESEARCH INSTITUTE FOR NONFERROUS METALS BEIJNG
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Problems solved by technology

[0002] When studying bacterial growth and ore leaching characteristics in the field of biometallurgy, the shake flask experiment is generally started. This experiment is simple to operate, easy to control, and has good stability, but the disadvantage is that it cannot be controlled and measured online.
However, due to the high leaching rate, the oxygen consumption rate of bacteria is high, so the dissolved oxygen concentration will become the rate-limiting step of leaching, so it is necessary to study the oxygen supply and consumption and mass transfer phenomena in the leaching process

Method used

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  • Bacterial agitation leaching experimental facility and method for measuring oxygen mass transfer coefficients
  • Bacterial agitation leaching experimental facility and method for measuring oxygen mass transfer coefficients
  • Bacterial agitation leaching experimental facility and method for measuring oxygen mass transfer coefficients

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Embodiment 1

[0027] Medium thermophilic bacteria (please refer to Chinese patent, its application number is 200610078976.6) stirring and leaching chalcopyrite concentrate, the mineral particle size is less than 30μm, the pulp concentration is 5% (m / v), and the aeration volume is 0.5m 3 Under the experimental conditions of / h, adjust the pH value of the ore slurry to the optimum pH value of bacteria (1.70-2.00); put the domesticated and cultured leaching bacteria in the exponential growth phase into the stirring tank, the inoculation rate is average Between 5% and 10% (v / v); start aeration, set the temperature at the optimum growth temperature for bacteria, start heating and measure bacterial growth, pH, ORP and DO;

[0028] After the bacterial growth lag period, the dissolved oxygen concentration of the leaching system will be maintained within a certain range. At this time, the aeration will be stopped, and the aeration will be resumed after the dissolved oxygen concentration drops to a ce...

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Abstract

The invention provides a bacterial agitation leaching experimental facility and a method for measuring and calculating oxygen mass transfer coefficients, bacterial oxygen consumption rate and saturated dissolved oxygen concentration. The experimental facility is suitable for bacterial agitation leaching in particular for the bacterial leaching at a high temperature, can provide online regulation control and measurement of temperature, pH, ORP and DO, and simultaneously has a real-time recording function. In the invention, a dynamic method in a fermentation technical research is used for measuring and calculating the oxygen mass transfer coefficients, the bacterial oxygen consumption rate and the saturated dissolved oxygen concentration. The bacterial agitation leaching experimental facility has the advantages of not only providing a bacterial agitation leaching device capable of performing online regulation control and recording correlation parameters, but also being suitable for researching the oxygen supply and consumption in the process of high temperature bacterial agitation leach, thereby providing a strong support for rate-limiting factors of high temperature bacterial leaching.

Description

technical field [0001] The invention relates to a bacterial stirring leaching experimental device and its method for measuring the oxygen mass transfer coefficient, in particular to the experimental device for stirring and leaching chalcopyrite concentrate with thermophilic bacteria and the method for measuring the oxygen mass transfer coefficient and the bacterial oxygen consumption rate. And the determination of saturated dissolved oxygen concentration. Background technique [0002] When studying bacterial growth and ore leaching characteristics in the field of biometallurgy, the shake flask experiment is generally started. This experiment is simple to operate, easy to control, and has good stability, but the disadvantage is that it cannot be controlled and measured online. Bacterial leaching of chalcopyrite has always been a difficult problem. There will be obvious passivation phenomenon when leaching with normal temperature bacteria or mesophilic bacteria, which will aff...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/02G01N33/48
CPCC12M27/02C12M41/12C12M41/32C12M41/26C12M41/28
Inventor 刘兴宇陈勃伟黄松涛刘文彦
Owner GENERAL RESEARCH INSTITUTE FOR NONFERROUS METALS BEIJNG
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