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Method for preparing nuciferine from lotus leaf

A technology of nuciferine and lotus leaf, which is applied in the field of preparing nuciferine to achieve the effects of large processing capacity, reduced dosage and less impurities

Inactive Publication Date: 2010-06-30
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method for the preparation of high-content nuciferine, such as "preparation of high-performance liquid chromatography for separation and purification of nuciferine" published by Liu Jingjing, the document discloses the method for preparing nuciferine by high-performance liquid phase. The product obtained by this method has high purity, but Only suitable for laboratory preparation

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  • Method for preparing nuciferine from lotus leaf

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Embodiment 1

[0030] Take lotus leaf, cut into shreds, take 10kg, add 100L 0.5% sulfuric acid solution, heat to 60°C and soak dynamically for 3 hours, filter, add 80L 0.5% sulfuric acid solution to the filter residue for extraction for 2 hours, filter, add 60L0.5% sulfuric acid solution to the filter residue % sulfuric acid solution for extraction for 1 hour, filter, combine the extracts, add 10% ammonia solution to adjust the pH to 9, concentrate to a density of 1.2, add chloroform to reflux for dissolution, and release the chloroform solution to decolorize through an activated carbon column to obtain a decolorized solution. Mix the sample with alumina, recover chloroform, then take 1500g alumina, pack it into a column, elute with a mixed solution of chloroform-methanol-1% ammonia water (28:8:1), monitor by thin layer, and finish washing until no nuciferine is found. Take off, collect the nuciferine fraction, recover the solvent to 1 / 3-1 / 4, place it for crystallization, and crystallize for ...

Embodiment 2

[0032] Take lotus leaves, cut them into shreds, take 10kg, add 100L of 0.5% hydrochloric acid solution, heat to 8°C and soak dynamically for 3 hours, filter, add 80L of 0.5% hydrochloric acid solution to the filter residue for extraction for 2 hours, filter, add 60L of 0.5% hydrochloric acid solution to the filter residue Extract with % hydrochloric acid solution for 1 hour, filter, combine the extracts, add 10% ammonia solution to adjust the pH to 12, concentrate to a density of 1.4, add chloroform to reflux for dissolution, and release the chloroform solution to decolorize through an activated carbon column to obtain a decolorized solution. Alumina was mixed and sampled, chloroform was recovered, and 1600g of alumina was taken again, packed into a column, eluted with a mixed solution of chloroform-methanol-1% ammonia water (28:8:1), and monitored by TLC until no nuciferine was removed. Take off, collect the nuciferine fraction, recover the solvent to 1 / 3-1 / 4, place to crystal...

Embodiment 3

[0034] Take lotus leaves, cut them into shreds, take 10kg, add 100L of 0.5% sulfuric acid solution, heat to 7°C and soak dynamically for 3 hours, filter, add 80L of 0.5% sulfuric acid solution to the filter residue for extraction for 2 hours, filter, add 60L of 0.5% sulfuric acid solution to the filter residue % sulfuric acid solution for extraction for 1 hour, filter, combine the extracts, add 10% ammonia solution to adjust the pH to 10, concentrate to a density of 1.32, add chloroform to reflux for dissolution, release the chloroform solution and decolorize it through an activated carbon column to obtain a decolorized solution, which is mixed with 100g120-200 Mix the sample with alumina, recover chloroform, then take 2000g alumina, pack it into a column, elute with a mixed solution of chloroform-methanol-1% ammonia water (28:8:1), monitor by TLC, and finish washing until no nuciferine is found. Take off, collect the nuciferine fraction, recover the solvent to 1 / 3-1 / 4, leave t...

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Abstract

The invention relates to a method for preparing nuciferine from lotus leaf; the process takes lotus leaf medical material as raw material and includes the steps that: the lotus leaf medical material is shredded, and is soaked in acid and extracted for 2-3 times, ammonia water is used for adjusting pH, and then the steps of concentration, chloroform dissolving, active carbon column decoloration, alumina column separation and chloroform-ethanol-ammonia water mixed solution elution are conducted to collect nuciferine fractions and recover reagents, and then the end products are obtained after placement crystallization, chloroform and ethanol recrystallization. The process is suitable for scaling up.

Description

Technical field: [0001] The invention relates to a method for preparing nuciferine from lotus leaves, in particular to a method for extracting and purifying by acid-dissolving and alkali-precipitating, and preparing nuciferine by alumina. Background technique: [0002] Nuciferine is a kind of aporphine alkaloid, hydrophobic weak base. [0003] Molecular formula: C19H21NO2. Molecular weight: 295.38 [0004] Molecular Structure: [0005] [0006] Properties: Nuciferine is a white needle-like crystal with a melting point of 161-164°C. [0007] Nuciferine is the main lipid-lowering active ingredient in lotus leaves, which has medicinal and dietary effects such as anti-free radicals, inhibition of hypercholesterolemia and arteriosclerosis. [0008] The lotus leaf is the dry leaf of the water lily family plant lotus (Nelumbo nucifera Gaertn.), which is semicircular or fan-shaped, and it is sub-circular after unfolding, entire or slightly wavy. The upper surface is dark gree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/18
Inventor 刘东锋张翼杨成东
Owner NANJING ZELANG MEDICAL TECH
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