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Process for production of EPA-enriched oil and DHA-enriched oil

一种油脂、工序的技术，应用在制造分别含有高浓度的二十碳五烯酸和二十二碳六烯酸浓缩油领域，能够解决双键数目多、氧化不稳定等问题，达到提高反应性的效果

Inactive Publication Date: 2010-06-30
NIPPON SUISAN KAISHA LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] PUFA is very unstable to oxidation due to the large number of double bonds

Method used

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  • Process for production of EPA-enriched oil and DHA-enriched oil
  • Process for production of EPA-enriched oil and DHA-enriched oil
  • Process for production of EPA-enriched oil and DHA-enriched oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Lipase-catalyzed reaction of step a)

[0040] Add Lipaz QLM 1.49g (100unit / g), water 20.7g, magnesium oxide (Junzheng Chemical Co., Ltd. special grade Reagent 99% or more, powder) 30g (equivalent to 2.5% of oil), ethanol 207ml, stirred at 40°C for 16 hours. After the reaction, the solid was separated by filtration, and washed with 560 ml of 20% sulfuric acid and 200 ml of saline, respectively. Then, the water and ethanol remaining in the oil layer were distilled off to obtain 1.21 kg of oil. A small amount of the obtained oil was taken and the glyceride fraction was separated by preparative TLC, and after methylation, the fatty acid component was analyzed by gas chromatography. The analysis conditions are as follows (unless otherwise specified, subsequent analyzes by preparative TLC and gas chromatography were performed in the same manner).

[0041] TLC separation and preparation conditions, methyl esterification conditions;

[0042] 1 ml of hexane and 10 ml of s...

Embodiment 2

[0072] The amount of Lipozyme TLIM (Lipozyme TLIM) used in step 3) of Example 1 was changed to 5 mg (equivalent to 0.5% of the oil), and the reaction was carried out under the same conditions as in step (3) of Example 1. The results are shown in Table 7 and Table 8. Even when the amount of lipase used is reduced, DHA concentrated oil and EPA concentrated oil can be simultaneously obtained. In this reaction, the recovery rate of DHA in the glyceride fraction was 114.7%, and the recovery rate of EPA in the ethyl ester fraction was 58.9%. (The numerical value exceeding 100% in the recovery rate is due to the simple calculation method of the recovery rate calculation method this time. That is, the measured value of the FID used to calculate the recovery rate, because the greater the relative sensitivity of the molecular weight, the greater the relative sensitivity, so for DHA Fatty acid with such a high molecular weight can obtain a higher value. It is the same in the following e...

Embodiment 3

[0079] DHA concentrated oil and EPA concentrated oil were prepared using low-temperature isolated and purified sardine oil with high EPA concentration (EPA 29.0%, DHA 12.5%, manufactured by Nippon Suisan Co., Ltd.) as a raw material lipid. The reaction of step a) is to use 1.2Kg of refined sardine oil, 1.49g of Lipaz QLM, 20.7ml of water, 60g of magnesium oxide, and 207ml of ethanol, and react at 40°C for 16 hours. The treatment operation is carried out in the same manner as in Example 1. Then, under the same conditions as in Example 1, the thin-film distillation process was performed twice to obtain a glyceride fraction. Using 1 g of the oil obtained in step a), 10 μL of water, 20 mg of Lipsem TLIM, 25 mg of magnesium oxide, and 173 μL of ethanol, the reaction of step b) was carried out, and the reaction was carried out at 40° C. for 16 hours. The ethyl ester and glyceride fractions were separated and prepared by preparative TLC (the same conditions as in Example 1). Raw mat...

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Abstract

Disclosed is a process for producing an EPA-enriched oil and a DHA-enriched oil simultaneously by: subjecting an oil-and-fat containing EPA and DHA to the alcoholysis with a lipase having a substrate specificity to a fatty acid having 18 or less carbon atoms in the presence of a reaction additive such as magnesium oxide; separating a glyceride fraction from the reaction solution; and subjecting the glyceride fraction to the alcoholysis with a lipase having a substrate specificity to a fatty acid having 20 or less carbon atoms in the presence of a reaction additive such as magnesium oxide.

Description

technical field [0001] The present invention relates to a method for producing concentrated oils containing high concentrations of eicosapentaenoic acid (hereinafter referred to as EPA) and docosahexaenoic acid (hereinafter referred to as DHA) by utilizing lipase catalyzed reaction. Background technique [0002] Eicosapentaenoic acid (hereinafter referred to as EPA) and docosahexaenoic acid (hereinafter referred to as DHA), which are n-3 polyunsaturated fatty acids (Polyunsaturated Fatty Acid, hereinafter referred to as PUFA), have various Physiological action, used as medicine, health food, food material, etc. EPA ethyl ester can be used as a therapeutic drug for arteriosclerosis and hyperlipidemia, and a drink to which fish oil containing EPA and DHA is added is also approved as a food for specified health use. In addition, the demand as a supplement in Japan and abroad is also very high. [0003] PUFA is very unstable to oxidation due to the large number of double bonds...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11C3/10C12P7/6458C12P7/6472
CPCC11C3/10C12P7/6472C11C1/045C12P7/6458A23D9/02C12P7/64
Inventor 降旗清代美川原裕之山口秀明池本英生土居崎信滋
Owner NIPPON SUISAN KAISHA LTD
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