Method for purifying active ingredients of compound Naodesheng

A purification method and technology of active ingredients, applied in the field of purification of active ingredients of compound Naodesheng, can solve problems such as being unfavorable to the development of traditional Chinese medicine, not in line with the modernization of traditional Chinese medicine, affecting the research of new traditional Chinese medicine compounds and the process of internationalization, etc. To achieve the effect of stable and uniform dry powder, shorten heating time and reduce loss

Inactive Publication Date: 2010-08-04
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It does not meet the requirements of the modernization of traditional Chinese medicine, is not conducive to the development of traditional Chinese medicine, and affects the research of new compound medicines of traditional Chinese medicine and the process of internationalization

Method used

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  • Method for purifying active ingredients of compound Naodesheng
  • Method for purifying active ingredients of compound Naodesheng
  • Method for purifying active ingredients of compound Naodesheng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The first part of the extraction process experiment

[0031] Experiments were carried out using the ethanol reflux extraction method, using L 9 (3 4 ) Orthogonal experimental method to investigate the process parameters of the first part of ethanol reflux extraction ethanol dosage (multiple of medicinal material quality), ethanol concentration, extraction time, extraction times and other factors, and use ultraviolet-visible spectrophotometry to analyze the total flavonoids and total flavonoids in the extract. The content of saponins was determined, and the contents of puerarin, ferulic acid, ginsenoside Rb1, ginsenoside Rg1 and notoginsenoside R1 were determined by high performance liquid chromatography at the same time, and these were used as indicators for comparative analysis. The results are shown in Table 1~Table 6.

[0032] Table 1 Level table of extraction process factors in the first part

[0033]

[0034]

[0035] Table 3 Orthogonal test resu...

Embodiment 2

[0051] Example 2 The second part of the extraction process experiment

[0052] (1) use the water bath temperature immersion extraction method to carry out experiments, adopt L 9 (3 4 ) Orthogonal experiment method to investigate the process parameters of the second part of water bath extraction (the multiple of medicinal material quality), extraction temperature, warm immersion time, leaching times and other factors, and adopt high performance liquid phase method to measure hydroxyl safflower yellow A content, and use this as an indicator for comparative analysis. The results are shown in Table 7~9.

[0053] Table 7 Safflower extraction process factor level table

[0054]

[0055] Table 8 Orthogonal test results of safflower extraction process

[0056]

[0057] Table 9 Analysis of variance

[0058]

[0059]

[0060] Orthogonal test results show (Table 8, Table 9): the amount of water added, extraction time, and extraction temperature all have a significant imp...

Embodiment 3

[0064] Embodiment 3 Resin purification process experiment of the first part

[0065] The extract obtained by extracting with the aforementioned optimal extraction conditions is carried out as follows:

[0066] (1) Screening of resin

[0067] For the 26 resin columns that have been processed: polyamide resin (including 80-100 mesh, 30-60 mesh, 14-30 mesh), macroporous adsorption resin (including D101, D101B, DM130, HP20, S-8, D4020 , NKA-9, HPD-100, AB-8, XDA-1, XDA-5, H-20, H-60, LSA-40), ion exchange resin (including anion and cation), epoxy resin, polyester Resin, natural resin, ketone-formaldehyde resin, phenolic resin, decolorizing resin, amino resin, etc., accurately absorb 26 parts of the first part of the extract, put it on the column, absorb it overnight, and then elute with distilled water and 70% ethanol in turn, and collect each part of the elution 26 samples were processed completely in parallel (injection speed 1ml / min, elution speed 2ml / min). The index compone...

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Abstract

The invention discloses a method for purifying active ingredients of compound Naodesheng. The method comprises the following steps: firstly dividing raw materials of the compound Naodesheng into two parts for screening, wherein the first part comprises sanchi, kudzuvine root, hawthorn fruit and Szechuan lovage rhizome, and the second part comprises safflower; carrying out extraction, concentration, vacuum filtration, concentration, absorption by resin and elution on the two parts, then extracting and purifying for obtaining purified eluate of the two parts, merging, decompressing, concentrating and drying for obtaining the active ingredients of the compound Naodesheng. The method for purifying the compound Naodesheng can effectively remove impurities without efficacy, ensure the yield, relatively improve the content of the active ingredients, improve the efficacy of the drug and realize very high using value.

Description

technical field [0001] The invention relates to the field of medicine purification, in particular to a method for purifying the active ingredients of compound Naodesheng. Background technique [0002] The compound Naodesheng is an effective traditional Chinese medicine compound. In 1979, it was approved by the Heilongjiang Provincial Department of Health to produce Naodesheng Pills and Naodesheng Tablets. , consisting of 78g of Panax notoginseng, 78g of Chuanxiong, 91g of safflower, 261g of kudzu root, and 157g of hawthorn (pitted) five herbs, it has the effects of promoting blood circulation and removing blood stasis, dredging meridians, refreshing the brain, and is used for cerebral arteriosclerosis, ischemic brain It has a remarkable curative effect on diseases such as stroke and cerebral hemorrhage sequelae. [0003] The pharmacological effects of each medicinal flavor of Naodesheng mainly include: each medicinal flavor in Naodesheng Compound has the effect of promoting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/734A61P9/10A61P25/00
Inventor 梁生旺王淑美王俊永李淑贤李明丽胡旭光
Owner GUANGDONG PHARMA UNIV
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