Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue

A periodontal tissue and biological technology, applied in the field of biodegradable periodontal tissue regeneration-promoting film preparation, can solve the problems of short half-life and inactivation of exogenous growth factors, and achieve good in vivo degradability and good biological phase. capacitive effect

Inactive Publication Date: 2010-08-04
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the half-life of exogenous growth factors is short, and they are quickly diluted and metabolized or inactivated by enzymes in the body when used locally. Only with the help of a suitable carrier can the growth factors continue to work efficiently and better promote tissue regeneration
[0004] In summary, in the reports of the existing literature, there is no information about the combination of biodegradable nanostructured membranes described in the present invention, and the controlled release of growth factors to inhibit periodontal tissue inflammation and regeneration. and induction to achieve relevant research reports on promoting periodontal tissue regeneration

Method used

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  • Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue
  • Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue
  • Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue

Examples

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Embodiment 1

[0045] Using PCL as a raw material, it was dissolved in chloroform to prepare a solution with a concentration of 15% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 10KV, and the collection distance to 15cm, a film composed of ultrafine fibers with nanometer-scale diameters can be obtained randomly, and then the The film was washed several times with deionized water and dried in vacuum before use. The film is first immersed in a solution of polyethyleneimine (PEI, 1-3 mg / ml) for 5-10 minutes, and then washed with deionized water with pH=7.0. Then BMP-2 and PDGF are made into a solution with a concentration of 0.1-10 μg / ml, and the film is soaked in the solution for 10-30 minutes for adsorption, and then washed with deionized water with pH=7.0. Then immerse in polyallylamine (PAH, 3mg / ml) and polyacrylic acid (PAA, 3mg / ml) solution in turn, cycle 3-4 times, all use deionized water t...

Embodiment 2

[0048] Using PLGA as a raw material, it was dissolved in chloroform to prepare a solution with a concentration of 20% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 20KV, and the collection distance to 20cm, a film composed of ultrafine fibers with nanometer-scale diameters can be obtained randomly, and then the The film was washed several times with deionized water and dried in vacuum before use. The film is first immersed in a solution of polyethyleneimine (PEI, 1-3 mg / ml) for 5-10 minutes, and then washed with deionized water with pH=7.0. Then BMP-2 and PDGF are made into a solution with a concentration of 0.1-10 μg / ml, and the film is soaked in the solution for 10-30 minutes for adsorption, and then washed with deionized water with pH=7.0. Then immerse in polyallylamine (PAH, 3mg / ml) and polyacrylic acid (PAA, 3mg / ml) solution in turn, cycle 3-4 times, all use deionized water ...

Embodiment 3

[0051] PLGA and collagen (collagen) were used as raw materials (2:1), dissolved in hexafluoropropanol to prepare a solution with a concentration of 10% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 15KV, and the collection distance to 15cm, and a film composed of ultrafine fibers with nanometer-scale diameters can be obtained randomly, and then the The film was washed several times with deionized water and dried in vacuum before use. Prepare BMP-2 and PDGF into a solution with a concentration of 0.1-10 μg / ml, soak the film in the solution for 10-30 minutes for adsorption, and then wash with deionized water with pH=7.0. Then immerse in polyallylamine (PAH, 3mg / ml) and polyacrylic acid (PAA, 3mg / ml) solution in turn, cycle 3-4 times, all use deionized water to elute after each deposition. Next, a P38 inhibitor (SB203580) and an ERK inhibitor (PD98059) were dissolved in dimethyl sul...

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Abstract

The invention relates to a preparation method of a biodegradable diaphragm for promoting regeneration of periodontal tissue, belonging to the field of biological regeneration medicine. In the invention, polylactic acid, polycaprolactone, polylactic acid-glycollic acid copolymer, collagen, modified chitosan and other biodegradable synthetic polyester polymers or natural biodegradable high molecular materials are used as raw materials to prepare a thin-film material with a nano structure through the electrostatic spinning process, and then, various growth factors are loaded on the thin-film material through the supermolecule loading technology. The diaphragm for reparative regeneration of the periodontal tissue, which is prepared by the method of the invention, has good biocompatibility and can realize the purposes of inhibiting inflammation and inducing regeneration of the periodontal tissue through the controlled release of the loaded growth factors.

Description

technical field [0001] The invention belongs to the field of bioregenerative medicine, and in particular relates to a preparation method of a biodegradable membrane for promoting periodontal tissue regeneration. Background technique [0002] Periodontal disease is one of the most common and most frequent diseases in humans, the most common of which is periodontitis. The significant pathological change of periodontal disease is the destruction of periodontal supporting tissue: alveolar bone absorption, connective tissue destruction attached to the bone, accompanied by different degrees of cementum absorption, if not treated effectively, it will lead to Teeth loosen and fall out. In the microbial milieu of the oral cavity, the major building blocks of bacteria, lipopolysaccharides derived from Gram-negative bacteria, can trigger periodontal disease-like irritative bone loss. LPS stimulates the expression of interleukin-1β, tumor necrosis factor-α, interleukin-6, and RNAKL by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/24A61L27/54
Inventor 赵鹏文学军张兵波林超时东陆
Owner TONGJI UNIV
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