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Establishment of rhesus autoimmune 1 type diabetes model

A technique for autoimmunity and type 1 diabetes, applied in the field of preparation of rhesus monkey autoimmune type 1 diabetes model

Active Publication Date: 2010-08-18
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The current literature reports the rhesus macaque animal model of insulin-dependent diabetes mellitus induced by STZ in the middle and high dose groups (45-60mg / Kg), and does not use low dose STZ (less than 30mg / Kg) for the preparation of autoimmune type 1 diabetes Related reports on primate models

Method used

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  • Establishment of rhesus autoimmune 1 type diabetes model
  • Establishment of rhesus autoimmune 1 type diabetes model
  • Establishment of rhesus autoimmune 1 type diabetes model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 animal model of the present invention

[0029] 1. Materials and methods:

[0030] 1.1 Experimental materials

[0031] Six 2-3-year-old female and male rhesus monkeys, free from B-herpes virus, simian retrovirus, simian leukemia virus and immunodeficiency virus infection; purchased from Chengdu Pingan Animal Breeding Base.

[0032] 1.2 Main reagents

[0033] Streptozotocin (STZ): purchased from Chengdu Yuyang High-tech Development Co., Ltd.

[0034] Other medicines and consumables were purchased from Likang Pharmacy of West China Hospital

[0035] 1.3 Preparation of the main solution

[0036] Preparation of streptozotocin solution:

[0037] 0.1mol / L sodium citrate buffer preparation:

[0038] Solution A (0.1mol / L citric acid solution): weigh 2.1g citric acid (C 6 h 8 o 7 ·H 2 O FW: 210.14) was dissolved in 100ml double distilled water. Solution B (0.1M sodium citrate solution): weigh 2.94g sodium citrate (C 6 h 5 Na 3 o 7 2H...

Embodiment 2

[0083] The drug intervention experiment of embodiment 2 animal model of the present invention

[0084] 1. Test drug: cyclosporine (immunosuppressive drug, which can reduce the incidence of autoimmune diabetes in mice);

[0085] 2. Test method: the intervention effect of cyclosporine on the pathogenesis of autoimmune diabetes

[0086] The experiment was divided into normal saline blank control group and cyclosporine intervention group: during the process of STZ-induced autoimmune diabetes, the intervention group was given cyclosporine (2.5mg / Kg) every day at the same time, and the comparison between the blank control group and the intervention group The onset time, incidence rate and damage degree of β cells of animal diabetes.

[0087]By interfering with the pathogenesis of autoimmune diabetes with immunosuppressive drugs, it was observed that the damage degree of β cells in the animal model was reduced, which further proves that the animal model of the present invention is a...

Embodiment 3

[0088] Example 3 Validate the rhesus monkey animal model of autoimmune type 1 diabetes with drugs for the treatment of autoimmune type 1 diabetes and preliminarily establish a related drug screening platform

[0089] At present, the treatment of autoimmune type 1 diabetes is mainly based on insulin injection. In this experiment, insulin was used in the rhesus monkey autoimmune type 1 diabetes model. The specific method is as follows:

[0090] 1. According to the habit of rhesus monkeys eating two meals a day, apply the method of injecting different doses of different types of insulin twice a day (9:30am&16:30pm) to the autoimmune type 1 diabetes model of the present invention prepared in Example 1: ①Long-acting insulin: Wanbang (Xuzhou) porcine insulin (protamine zinc insulin) and ②Short-acting insulin: Wanbang (Xuzhou) porcine ordinary insulin combined treatment;

[0091] 2. In order to prevent hypoglycemia, start subcutaneously injecting insulin with a small dose (0.4U / Kg); ...

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Abstract

The invention provides application of streptozotocin with low dosage in preparing an animal model for sieving drugs for treating autoimmune 1 type diabetes. The application method and dosage of the streptozotocin is 15-30mg / kg once by veins administration. The invention also provides method for preparing a rhesus autoimmune 1 type diabetes model, and autoimmune 1 type diabetes animal model prepared by the method. The animal model can be used for the evaluation of the biotechnology new drug which can not be evaluated by the rodent animal model, and the evaluation of the stem cell transplantation treating technology.

Description

technical field [0001] The invention relates to a method for preparing a rhesus monkey autoimmune type 1 diabetes model. Background technique [0002] The etiology of autoimmune diabetes type 1 diabetes is unknown, and there is no effective preventive method so far. Although commonly used insulin therapy can regulate blood sugar levels to a certain extent, long-term use cannot effectively control the occurrence of diabetic complications, and some patients may suffer from hypoglycemic shock. How to prevent the autoimmune system from attacking islet β-cells and how to effectively transplant β-cells has become a key issue in diabetes research. [0003] At present, the most commonly used model animals for diabetes research are rodents. Because rodents are far away from humans in evolutionary relationship, the experimental data obtained with rodents as research objects have little clinical significance. The anatomical characteristics, physiological functions of various systems...

Claims

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Application Information

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IPC IPC(8): A61K49/00A01K67/027
Inventor 陆燕蓉程惊秋邓绍平陈又南田伯乐麦刚任艳王莉李宏霞魏玲玲金熙乔超锋张文胜何斯荣曾力
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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