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Method for producing food rich in gamma-aminobutyric acid

A production method and aminobutyric acid technology are applied in food preparation, food science, application and other directions, can solve problems such as low glutamic acid conversion rate, long production cycle, etc., and achieve convenient GABA content control, simple production process, and glutamate The effect of rapid acid decarboxylation reaction

Inactive Publication Date: 2012-11-14
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the technical purpose of the present invention is to provide a new production method of GABA-rich food material, which can overcome the low glutamic acid conversion rate and long production cycle in the single material enrichment method or fermentation method of the prior art. problems, and can efficiently produce GABA-rich ingredients to meet the special requirements of various groups of people

Method used

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  • Method for producing food rich in gamma-aminobutyric acid
  • Method for producing food rich in gamma-aminobutyric acid
  • Method for producing food rich in gamma-aminobutyric acid

Examples

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Effect test

Embodiment 1

[0038] (1) Weigh 5g of rice germ, crush it through a 40-mesh sieve, put it in a 100mL conical flask (label 1), add acetic acid-sodium acetate buffer solution with a pH of 3.5 according to the ratio of solid to liquid at 1:5; place the conical flask in Soak in a water bath at 10°C for 0.5h, and keep stirring to activate glutamic acid decarboxylase; (2) Weigh 5g of peanuts, crush them through a 40-mesh sieve; put them in a 100mL conical flask (label 2), and Add acetic acid-sodium acetate buffer solution at a ratio of 1:5; put the triangular flask in a water bath at 10°C for 1 hour, and keep stirring, and use endogenous enzyme (protease) to hydrolyze the protein; (3) 1 Mix the feed liquid of the No. 2 Erlenmeyer flask with the feed liquid of the No. 2 Erlenmeyer flask in a 250mL Erlenmeyer flask, add calcium chloride 1mmol / mol-glutamic acid; then place the 250mL Erlenmeyer flask in a shaking table at 10°C for oscillation reaction 0.5h; (4) Measure the GABA content of the raw mate...

Embodiment 2

[0040] (1) Weigh 2g of barley germ, crush it through a 40-mesh sieve, put it in a 100mL conical flask (label 1), add acetic acid-sodium acetate buffer solution with a pH of 5.0 according to the ratio of solid to liquid 1:10; place the conical flask in Soak in a water bath at 30°C for 6 hours, and keep stirring to activate glutamic acid decarboxylase; (2) Weigh 10g of soybeans, crush them through a 40-mesh sieve; place them in a 100mL conical flask (label 2), and Add acetic acid-sodium acetate buffer solution with pH 5.0 at 1:10; soak the triangular flask in a water bath at 30°C for 12 hours, and keep stirring, and use endogenous enzyme (protease) to hydrolyze the protein; (3) No. 1 The feed liquid of the Erlenmeyer flask and the feed liquid of the No. 2 Erlenmeyer flask were mixed in a 250mL Erlenmeyer flask, and calcium chloride 15mmol / mol-glutamic acid was added; then the 250mL Erlenmeyer flask was placed in a shaker at 30°C for oscillating reaction for 6h (4) measure the GA...

Embodiment 3

[0042] (1) Weigh 1g of banana, crush it through a 40-mesh sieve, put it in a 100mL conical flask (label 1), add acetic acid-sodium acetate buffer solution with a pH of 6.5 according to the ratio of solid to liquid at 1:15; place the conical flask at 50 Soak in a water bath at ℃ for 12 hours, and keep stirring to activate glutamic acid decarboxylase; (2) Weigh 10g of corn, crush it through a 40-mesh sieve; : 15 add the acetic acid-sodium acetate buffer solution of pH6.5; Soak the triangular flask in the water bath of 70 ℃ for 48h, and constantly stir, utilize endogenous enzyme (protease) to make protein hydrolysis; (3) No. 1 triangular flask The feed liquid of the bottle and the feed liquid of the No. 2 Erlenmeyer flask were mixed in a 250mL Erlenmeyer flask, and calcium chloride 50mmol / mol-glutamic acid was added; then the 250mL Erlenmeyer flask was placed in a shaker at 50°C for oscillating reaction for 12h; (4) Measure the GABA content of the raw material before and after th...

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Abstract

The invention provides a method for producing food rich in gamma-aminobutyric acid (GABA). In the method, food rich in glutamate decarboxylase and food rich in glutamic acid are mixed to undergo a decarboxylic reaction to give the food rich in GABA. The method of the invention can solve the problems such as low glutamic acid conversion rate and long production period of a single-material concentration method or fermentation method in the prior art and effectively produce the food rich in GABA to meet special demands of all kinds of people.

Description

technical field [0001] The invention relates to a production method of a food material rich in gamma-aminobutyric acid, in particular to a method for producing a food material rich in gamma-aminobutyric acid by a multi-material enzymatic method. Background technique [0002] γ-aminobutyric acid (molecular formula HOOCCH 2 CH 2 CH 2 NH 2 , molecular weight 103.12, hereinafter referred to as GABA), also known as aminobutyric acid, piperidine acid. GABA is a non-protein functional amino acid that widely exists in the animal and plant kingdoms. It is an inhibitory neurotransmitter in the cerebrospinal cord of mammals. It can activate glucose metabolism in the brain and promote the synthesis of acetylcholine. It plays an important role in the energy metabolism of the human brain. In medicine, the main effects of GABA are: treating symptoms caused by cerebrovascular disorders such as hemiplegia, memory impairment, children's mental retardation, mental immaturity, etc.; improv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/29A23L1/48A23L33/00A23L35/00
Inventor 缪冶炼戴凤燕何珣
Owner NANJING TECH UNIV