Application of forsythiaside A in preparation of drugs for preventing and treating cardiovascular and cerebrovascular diseases

A technique for cardiovascular and cerebrovascular diseases and forsythiaside is applied in the application field of forsythiaside A in the preparation of drugs for preventing and treating cardiovascular and cerebrovascular diseases, which can solve the problems of never-before-seen drugs for cardiovascular and cerebrovascular diseases, and achieve strong pharmacological effects Effect

Active Publication Date: 2010-09-01
山西华卫药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to reports, forsythiaside A has antiviral, antibacterial, antipyretic, analgesic, hepatoprotective, antioxidative, anti-inflammatory and anti-en

Method used

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  • Application of forsythiaside A in preparation of drugs for preventing and treating cardiovascular and cerebrovascular diseases
  • Application of forsythiaside A in preparation of drugs for preventing and treating cardiovascular and cerebrovascular diseases
  • Application of forsythiaside A in preparation of drugs for preventing and treating cardiovascular and cerebrovascular diseases

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1 Effect of forsythoside A on ADP-induced platelet aggregation in rats

[0014] Blood taken from the abdominal aorta of rats (Wistar, clean level II), 3.8% sodium citrate 1:9 anticoagulation, 500r·min -1 Centrifuge for 5 min to take the supernatant to obtain platelet-rich plasma (PRP). The remaining part 2500r·min -1 After centrifugation for 10 min, the supernatant was taken to obtain platelet-poor plasma (PPP). Dilute PRP with PPP to make the platelet count to 3×10 11 / L.

[0015] Take 265μL of PRP and place it in a turbidimetric tube, and add forsythiaside (content greater than 95%) solution (final concentration is 2.1, 2.6, 3.1, 3.6, 4.1, 6.2, 8.2mmol·L) -1 ) 20μL, add equal volume of saline to the blank control tube, pre-warm at 37°C for 3min, start the platelet aggregation and coagulation factor analyzer, and quickly add 2mmol·L -1 ADP15μL, record the platelet aggregation curve, and calculate the inhibition rate of drug platelet aggregation by the following for...

Example Embodiment

[0022] Example 2 Effect of forsythoside A on rat plasma prothrombin time (PT)

[0023] Take blood from the abdominal aorta of rats, 3.8% sodium citrate 1:9 anticoagulation, 2500r·min -1 After centrifugation for 10 min, the supernatant was taken to obtain PPP. Pre-warm the PT reagent in the reagent pre-warming hole at 37°C for more than 10 minutes, and shake it well before use. Take 50μL of PPP into a turbidimetric tube, add forsythiaside A solution (final concentration is 8.3, 12.5, 16.6mmol·L -1 ) 30μL, add an equal volume of saline to the control tube, add 100μL of PT reagent at 37°C for 180s after accurate prewarming at 37°C, immediately start the instrument for automatic testing, and record the results.

[0024] The results showed that: forsythiaside can significantly prolong the PT of rat plasma in vitro, and the prolongation effect becomes more obvious with the increase of dose (Table 2). The final concentration is 16.6mmol·L -1 The effect of forsythoside prolonging PT has b...

Example Embodiment

[0028] Example 3 Effect of forsythoside A on rat plasma thrombin time (TT)

[0029] The PPP preparation method is the same as above. Take the TT reagent out of the refrigerator and let it stand at room temperature for 10 minutes, and the temperature is balanced to room temperature. Take 80μL of PPP into a turbidimetric tube, add forsythiaside solution (final concentration of 7.1, 10.7, 14.2mmol·L -1 ) 30μL, add an equal volume of saline to the control tube, add 100μL of TT reagent after accurate prewarming at 37°C for 180s, immediately start the instrument for automatic testing, and record the results.

[0030] The results showed that: Forsythoside A can significantly prolong the TT of rat isolated plasma, and there is a significant dose-effect relationship (Table 3).

[0031] Table 3 Effects of forsythiaside A on rat plasma TT in vitro ( n=6)

[0032]

[0033] *P<0.05 vs NS,***P<0.001 vs NS

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Abstract

The invention proves that forsythiaside A has the function of inhibiting platelet aggregation and simultaneously has the obvious anticoagulation role by studying the impacts of the forsythiaside A on ADP-induced platelet aggregation in vitro of rats, the impacts on plasma prothrombin time (PT) of the rats and the impacts on plasma thrombin time (TT) of the rats. The forsythiaside A can be used for preparing drugs for preventing and treating cardiovascular and cerebrovascular diseases. The forsythiaside A can also be used as a food additive for preparing functional foods for preventing and treating the cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention relates to forsythiaside A, specifically the application of forsythiaside A in the preparation of drugs for preventing and treating cardiovascular and cerebrovascular diseases. Background technique [0002] Forsythoside A (Forsythoside A) can be obtained by extracting Forsythia Suspensa (Thunb.) Vahl. from Oleaceae. The structural formula is: [0003] [0004] Molecular formula C 29 h 36 o 15 , with a molecular weight of 624, light yellow powder compound, easily soluble in water, ethanol, methanol, etc. [0005] It is reported that forsythiaside A has antiviral, antibacterial, antipyretic, analgesic, hepatoprotective, antioxidative, anti-inflammatory and anti-endotoxin activities. However, there has never been any report on the preparation of drugs for the prevention and treatment of cardiovascular and cerebrovascular diseases. Contents of the invention [0006] The purpose of the present invention is to expand the use of forsyt...

Claims

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Application Information

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IPC IPC(8): A61K31/7032A61P9/00A61P7/02A61P9/10A23L1/30A23L33/105
Inventor 张立伟柴秋彦
Owner 山西华卫药业有限公司
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