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Method for enhancing stability of beer brewing technique and dedicated complex enzyme thereof

A compound enzyme and stability technology is applied in the field of improving the stability of beer brewing process and its special compound enzyme, which can solve the problems of hindering filtration speed, loss of wort extract content, slow filtration speed, etc. The effect of beer quality, simple and convenient operation

Active Publication Date: 2010-09-08
北京挑战生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional beer brewing only uses the enzyme system produced when barley germinates. Some endogenous enzymes of malt (such as β-glucanase, cellulase, hemicellulase, etc.) During the drying process, these enzymes are damaged to varying degrees, which reduces the solubility of the malt, resulting in a series of problems in beer brewing, mainly: the insoluble β-glucan and pentosan in the malt not only Become a viscous obstacle during filtration, causing slow filtration speed in the filtration process, easy oxidation of wort, and loss of extract content in wort; residues of β-glucan cause beer turbidity and reduced foam persistence One of the main reasons for the weak cup-hanging force; the protein in the raw material cannot be effectively decomposed, which hinders the filtration speed during denaturation and precipitation, and makes the content of α-amino nitrogen in the wort low, resulting in low fermentation degree of the wort
These will have a serious impact on the stability of the beer brewing process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Protocol Method Saccharification and Enzyme Test

[0023] The saccharification and enzyme test was carried out according to the protocol method in "Beer Analysis and Detection Technology" (Chemical Industry Press, Editor-in-Chief Dong Xiaolei, July 2008).

[0024] Weigh 50.0g of crushed malt into a saccharification cup of known quality, weigh 0.01g of the complex enzyme provided by the present invention, dissolve it with a small amount of warm water and add it to the saccharification cup, add 200mL of distilled water (generally 46~47℃), After mixing, the temperature of the solution just reached about 45°C, and the temperature was kept at 80-100 rpm for 30 minutes. Among them, the name of each component and the enzyme activity of each component in the composite enzyme used are: temperature-resistant β-glucanase: 4000-4500 U / g; cellulase (from exo β-1, 4-glucan Enzyme, endo β-1,4-glucanase and β-1,4-glucosidase; purchased from Zhaodong Richeng Enzyme Preparation Co...

Embodiment 2、100

[0033] Example 2. Application test of adding enzyme in 100L saccharification tank

[0034] Using malt as the main raw material, supplemented by 25% of the total raw material weight of rice, the test was carried out in a 100L saccharification tank.

[0035] Weigh 15kg of malt, add it to the saccharification tank after crushing, weigh 3g of the compound enzyme provided by the invention, dissolve it with a small amount of warm water and add it to the saccharification tank; weigh 5kg of rice, crush it, add it to the gelatinization pot, add 1.25mL α- Amylase (20000U / mL); Among them, the addition of α-amylase can liquefy the starch in the rice, which is beneficial to transport the mash from the gelatinization pot to the saccharification pot, and improves the conversion rate of the starch in the rice to fermentable sugar. The experiment was carried out in accordance with the double mash twice boiling saccharification method provided in the "Beer Industry Manual (Revised Edition)" (Editor ...

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Abstract

The invention discloses a method for enhancing the stability of the beer brewing technique and a dedicated complex enzyme thereof. Each gram of complex enzyme contains the following components by enzyme activity (U / g): 3000 to 5000 heat-resistant Beta-glucanase, 1000 to 2500 cellulase, 3000 to 4500 xylanase, 20000 to 25000 neutral protease and 10000 to 14000 heat-resistant Alpha-amylase. The method adds the complex enzyme into a saccharifying tank at the beginning of saccharification. When the complex enzyme is applied to the saccharification process of beer brewing, the content of Alpha-amino nitrogen and the total content of nitrogen in beerwort can be increased, the filtration rate and fermentability of beerwort and beer can be increased, consequently, the stability of the beer brewingtechnique is ensured, and the quality of beer is increased. The method is mainly applied to the saccharification process of beer brewing, and the operation is simple and convenient.

Description

Technical field [0001] The invention relates to a method for improving the stability of a beer brewing process and a special composite enzyme, in particular to a method for improving the stability of the beer brewing process by using an enzyme preparation as a saccharification accelerator. Background technique [0002] In addition to 60-70% starch, barley malt also contains protein, cellulose, β-glucan, pentosan and other substances. Traditional beer brewing only uses the enzymes produced during the sprouting of barley. Some endogenous enzymes in malt (such as β-glucanase, cellulase, hemicellulase, etc.) synthesize enzyme activity during the sprouting of barley. During the drying process, these enzymes are destroyed to varying degrees, which reduces the solubility of malt, resulting in a series of problems in beer brewing, mainly: the insoluble β-glucan and pentosan in malt are not only It becomes a sticky obstacle during filtration, which causes slow filtration during the filtr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12C7/04C12N9/46C12N9/42C12N9/24C12N9/26
Inventor 吴培均王鹏银李富伟黄辉
Owner 北京挑战生物技术有限公司
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