Method for enhancing stability of beer brewing technique and dedicated complex enzyme thereof
A compound enzyme and stability technology is applied in the field of improving the stability of beer brewing process and its special compound enzyme, which can solve the problems of hindering filtration speed, loss of wort extract content, slow filtration speed, etc. The effect of beer quality, simple and convenient operation
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Embodiment 1
[0022] Example 1. Protocol Method Saccharification and Enzyme Test
[0023] The saccharification and enzyme test was carried out according to the protocol method in "Beer Analysis and Detection Technology" (Chemical Industry Press, Editor-in-Chief Dong Xiaolei, July 2008).
[0024] Weigh 50.0g of crushed malt into a saccharification cup of known quality, weigh 0.01g of the complex enzyme provided by the present invention, dissolve it with a small amount of warm water and add it to the saccharification cup, add 200mL of distilled water (generally 46~47℃), After mixing, the temperature of the solution just reached about 45°C, and the temperature was kept at 80-100 rpm for 30 minutes. Among them, the name of each component and the enzyme activity of each component in the composite enzyme used are: temperature-resistant β-glucanase: 4000-4500 U / g; cellulase (from exo β-1, 4-glucan Enzyme, endo β-1,4-glucanase and β-1,4-glucosidase; purchased from Zhaodong Richeng Enzyme Preparation Co...
Embodiment 2、100
[0033] Example 2. Application test of adding enzyme in 100L saccharification tank
[0034] Using malt as the main raw material, supplemented by 25% of the total raw material weight of rice, the test was carried out in a 100L saccharification tank.
[0035] Weigh 15kg of malt, add it to the saccharification tank after crushing, weigh 3g of the compound enzyme provided by the invention, dissolve it with a small amount of warm water and add it to the saccharification tank; weigh 5kg of rice, crush it, add it to the gelatinization pot, add 1.25mL α- Amylase (20000U / mL); Among them, the addition of α-amylase can liquefy the starch in the rice, which is beneficial to transport the mash from the gelatinization pot to the saccharification pot, and improves the conversion rate of the starch in the rice to fermentable sugar. The experiment was carried out in accordance with the double mash twice boiling saccharification method provided in the "Beer Industry Manual (Revised Edition)" (Editor ...
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