Method for producing high-purity oligomate

A high-purity galacto-oligosaccharide technology, applied in oligosaccharides, chemical instruments and methods, and sugar derivatives, can solve the problems of low separation efficiency, large mobile phase consumption, and high production costs in fixed-bed chromatography. Achieve the effects of low production cost, increased yield and high utilization rate

Inactive Publication Date: 2010-09-15
方煜宇
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  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantages of fixed-bed chromatography, such as low separation efficiency, low product

Method used

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  • Method for producing high-purity oligomate
  • Method for producing high-purity oligomate
  • Method for producing high-purity oligomate

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The feeding material is lactose solution with a concentration of 300g / L passing through the immobilized enzyme reaction column equipped with β-galactosidase, the reaction temperature is 50°C, and the reaction pH is 5.0. The content of galacto-oligosaccharides in the outflowing enzyme conversion liquid is 45%, lactose 18%, galactose 12%, and glucose 25%. The enzyme conversion solution was passed through simulated moving bed chromatography, and the operating conditions of the simulated moving bed chromatography system were as follows: separation temperature 60°C, feed flow rate 1 liter / hour, eluting water flow rate 1.7 liters / hour, system operating pressure 1.0MP, feed Reach stable after 20 hours, the discharge situation that obtains is as follows:

[0045] 1) Galacto-oligosaccharide part: the dry matter concentration is about 13% (~1.1 times the volume of the feed); the purity of galacto-oligosaccharide is 87.2%, lactose is 11.0%, galactose is 0.5%, and glucose is 1.0%. ...

Embodiment 2

[0048] The feed is that lactose solution with a concentration of 400g / L passes through the immobilized enzyme reaction column equipped with β-galactosidase, the reaction temperature is 55°C, and the reaction pH is 5.5. The content of galacto-oligosaccharides in the outflowing enzyme conversion solution was 56%, lactose 18%, galactose 4%, and glucose 22%. The enzyme conversion solution was passed through simulated moving bed chromatography, and the operating conditions of the simulated moving bed chromatography system were as follows: separation temperature 65°C, feed flow rate 1 liter / hour, eluting water flow rate 2.9 liters / hour, system operating pressure 1.1MP, feed Reach stable after 20 hours, the discharge situation that obtains is as follows:

[0049] 1) Galacto-oligosaccharide part: the dry matter concentration is about 14% (~2.8 times the volume of the feed); the purity of galacto-oligosaccharide is 92%, lactose is 6.1%, galactose is 0.3%, and glucose is 1.3%.

[0050]...

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Abstract

The invention discloses a method for producing high-purity oligomate. The high-purity oligomate is prepared by separating from a liquid of enzymatical reaction of the oligomate by synthesizing a special resin and using a simulated mobile bed chromatographic technique. The method comprises the specific steps of: 1), producing a mixture containing glucose, galactose, lactose and the oligomate in the presence of beta galactosidase by taking the lactose as a raw material; and 2), by using a simulated mobile bed chromatographic system, separating the oligomate from other ingredients completely by taking water as an eluent at the operating temperature of between 55 and 75 DEG C to obtain the oligomate-containing components and other miscellaneous components.

Description

technical field [0001] The invention relates to a preparation method of high-purity galacto-oligosaccharides, in particular, it relates to clever use of special resin and simulated moving bed technology to separate and prepare high-purity galacto-oligosaccharides from enzyme conversion liquid of low-purity galacto-oligosaccharides. Background technique [0002] Galacto-oligosaccharides (Galacto-Oligosaccharides, GOS) is a functional oligosaccharide with natural properties, and its molecular structure is generally 2 to 5 galactosyl groups connected to glucose or lactose molecules, that is, Glu-(Gal )n, (n=2~5). In nature, there are trace amounts of GOS in animal milk, but more in human breast milk. The establishment of bifidobacteria in infants largely depends on the GOS components in breast milk. Because of this, galacto-oligosaccharides are one of the most attractive functional oligosaccharides. Galacto-oligosaccharides have many beneficial functions, such as promoting th...

Claims

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Application Information

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IPC IPC(8): C12P19/14C07H3/06C07H1/08
Inventor 方煜宇章云
Owner 方煜宇
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