Process for producing D(-)-tartaric acid by biotransformation

A biotransformation and tartaric acid technology, applied in the field of bioengineering, achieves the effects of good performance, reduced production costs, and reduced equipment volume

Inactive Publication Date: 2010-09-29
ZHEJIANG GENEBEST PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no effective production process for the biological conversion of epoxysuccinic acid to D(-)-tartaric acid

Method used

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  • Process for producing D(-)-tartaric acid by biotransformation
  • Process for producing D(-)-tartaric acid by biotransformation

Examples

Experimental program
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Effect test

Embodiment

[0011] 1. Purification of cis-epoxysuccinate hydrolase

[0012] Use a strain (Bordetella sp.Strain 1-3) producing epoxysuccinate hydrolase, ferment in a 10-liter fermenter at 30°C for 30 hours, then centrifuge at 8000rpm for 20min to collect the bacteria, and use 50mmol / l pH 7.5 Cells were washed once with phosphate buffered saline. The formula of the fermentation broth is: yeast extract 7.8g / l, cis-epoxysuccinic acid 9.8g / l, KH 2 PO 4 1.12g / l, K 2 HPO 4 ·3H 2 O 3g / l, MgSO 4 ·7H 2 O 0.625g / l, trace element solution 12.5ml / l, pH 7.0. Afterwards, the obtained cells were suspended in 2 times the volume of 50 mmol / l pH 7.5 phosphate buffer, the cells were broken with a high-pressure homogenizer, and the cell wall fragments were removed by centrifugation at 8000 rpm for 20 min to obtain cis Crude extract of epoxysuccinate hydrolase.

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Abstract

The invention provides a process for producing a D(-)-tartaric acid by biotransformation. The process comprises the following steps of: A, extracting cis-epoxysuccinic acid hydrolase from microorganisms capable of degrading epoxysuccinic acid, and purifying the cis-epoxysuccinic acid hydrolase; B, immobilizing the purified cis-epoxysuccinic acid hydrolase; C, performing catalytic hydrolysis of the epoxysuccinic acid by using the immobilized enzyme as a biocatalyst to produce the D(-)-tartaric acid. By employing the properly purified enzyme for immobilization, the specific activity of the catalytic is increased greatly, so the reaction time and required equipment volume are reduced and consequently the production cost is reduced. The method for immobilizing the enzyme combines a carrageenan immobilizing method with strong mechanical strength and a protein (gelatin and enzyme) covalent crosslinking method with strong stability and toughness, so the immobilized enzyme has better service performance and a longer service life. As a result, the production cost of the D(-)-tartaric acid is reduced.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a production process of D(-)-tartaric acid, in particular to a process for producing D(-)-tartaric acid through biotransformation with epoxysuccinate hydrolase. Background technique [0002] Like L(+)-tartaric acid has many important uses, D(-)-tartaric acid has an irreplaceable role in food, medicine, chemical industry and light industry. D(-)-tartaric acid has a stronger sour taste than citric acid. Therefore, it can be used as a food sour agent, as well as a stabilizer and preservative. The left-handed optical activity allows D(-)-tartaric acid to act as a chiral resolving agent in the pharmaceutical industry and light industry. There have been many reports in this regard. The production method of L(+)-tartaric acid has been studied quite thoroughly, and the technology of producing L(+)-tartaric acid by biotransformation is also very mature, and large-scale industrial production h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12N11/02C12R1/40C12R1/05C12R1/01
Inventor 宋苗根
Owner ZHEJIANG GENEBEST PHARMA
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