Method for preparing artificial bone from chitosan and shell powder serving as raw materials

A technology of shell powder and chitosan, which is applied in the preparation of artificial bone and in the field of artificial bone preparation, can solve the problems of difficult shape maintenance, poor mechanical strength, and difficulty in reshaping, etc., and achieve the effect of improved molecular structure and excellent mechanical properties

Inactive Publication Date: 2010-10-13
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to poor mechanical strength, the shape is not easy to maintain after implantati

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Preparation of artificial bone

[0035] (1) Preparation of chitosan solution

[0036] Take by weighing 12.5 grams of chitosan and place it in a 500 milliliter beaker, add 250 milliliters of mass ratio concentration and be 1% acetic acid solution, seal the mouth of the beaker with kraft paper, put it in an oven (40 ° C,), after the chitosan is completely dissolved take out.

[0037] (2) Preparation of mixed solution

[0038] Take by weighing 112.5 grams of shell powder, add in the chitosan solution, and mechanically stir until the shell powder is evenly dispersed in the chitosan solution.

[0039] (3) Molding

[0040] Pour the above-mentioned mixed solution into the above-mentioned mold, put it in an oven (40° C.), and after hardening to a certain extent, destroy and remove the mold, and freeze-dry.

[0041] (4) Cross-linking

[0042] Put the above materials in a large beaker, add 500 ml of glutaraldehyde, seal the mouth of the beaker with kraft paper, and let it...

Embodiment 2

[0074] 1) Preparation of artificial bone

[0075] (1) Preparation of chitosan solution

[0076] Take by weighing 6 grams of chitosan and place it in a 200 milliliter beaker, add 60 milliliters of acetic acid solution with a mass ratio concentration of 3%, seal the mouth of the beaker with kraft paper, put it into an oven (40 ° C,), and wait for the chitosan to dissolve completely Then take it out.

[0077] (3) Preparation of mixed solution

[0078] Weigh 24 grams of shell powder, add it to the chitosan solution, and stir mechanically until the shell powder is evenly dispersed in the chitosan solution.

[0079] (4) forming (as embodiment 1)

[0080] (5) Cross-linking

[0081] Put the above materials in a large beaker, add 100 ml of glutaraldehyde, seal the mouth of the beaker with kraft paper, and let it stand for 24 hours.

[0082] (6) rinsing (as in Example 1)

[0083] (7) Plastic surgery

[0084] Take out the material and cut it into a cuboid of 30mm (width) × 30mm (h...

Embodiment 3

[0091] 1) Preparation of artificial bone

[0092] (1) Preparation of chitosan solution

[0093] Take by weighing 8 grams of chitosan and place it in a 200 milliliter beaker, add 100 milliliters of acetic acid solution with a mass ratio concentration of 5%, seal the mouth of the beaker with kraft paper, put it into an oven (40 ° C,), and wait for the chitosan to dissolve completely Then take it out.

[0094] (3) Preparation of mixed solution

[0095] Take by weighing 42 grams of shell powder, add in the chitosan solution, and mechanically stir until the shell powder is evenly dispersed in the chitosan solution.

[0096] (4) forming (as embodiment 1)

[0097] (5) Cross-linking

[0098] Put the above materials in a large beaker, add 100 ml of glutaraldehyde, seal the mouth of the beaker with kraft paper, and let it stand for 24 hours.

[0099] (6) rinsing (as in Example 1)

[0100] (7) Plastic surgery

[0101] Take out the material and cut it into a cuboid of 40mm (width) ...

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Abstract

The invention discloses a method for preparing an artificial bone from chitosan and shell powder serving as raw materials. The chitosan is the medical class chitosan in the markets abroad and at home, and has the deacetylated degree not less than 85 percent; and the shell powder is the residue after stratum corneum of anodonta woodiana is removed, and contains a shell prismatic layer and a pearl layer. The prepared artificial bone has the flexural modulus between 250 and 900Mpa, the compression modulus between 500 and 1,300MPa and the Young modulus between 160 and 280MPa. Meanwhile, the cellular structure in the artificial bone can meet the requirement of cell growth. The porosity, pore size, bioviscoelastic mechanics parameter and the like can be adjusted according to the ratio of the chitosan to the shell powder. The artificial bone is suitable for filling a bone defect part, and the mechanism of the artificial bone is osteoconduction, namely the artificial bone induces bone formation by promoting the combination of host bone and artificial bone surfaces.

Description

technical field [0001] The invention relates to the technical fields of biological tissue engineering and biological materials, in particular to a method for preparing artificial bone, specifically a method for preparing artificial bone by using chitosan and shell powder as raw materials. Background technique [0002] Bone defect is a common clinical case in orthopedic surgery, usually treated with autologous bone graft, allogeneic bone graft, free bone graft with blood supply, etc. However, these methods are generally restricted by the source of materials. Therefore, people try to solve the problem of repairing bone defects through tissue engineering. Artificial bone substitute material is a research hotspot in recent years. [0003] The working principle of artificial bone includes three aspects. ①Osteogenesis: The artificial bone material contains living cells with the potential to differentiate into bone, and has bone formation; ②Osteoconduction: The artificial bone ma...

Claims

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Application Information

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IPC IPC(8): A61L27/44
Inventor 刘彬张耀光王志坚蒲德永戴丽
Owner SOUTHWEST UNIVERSITY
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