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Application of quinine compound in preparing anti-tubercle bacillus drugs

A technology of anti-tuberculosis bacteria and compounds, applied in the direction of antibacterial drugs, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of non-standard treatment and management of tuberculosis patients, difficulties in tuberculosis prevention and control, and achieve the goal of expanding types Effect

Inactive Publication Date: 2012-02-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the irregular treatment and management of tuberculosis patients, irregular chemotherapy and abuse of anti-tuberculosis drugs, the drug resistance of tuberculosis is becoming more and more serious, and the change of drug resistance tends to be multi-drug resistance at the same time. great difficulty at work
At present, there is no report about the anti-tuberculosis activity of 1403B and 1403C

Method used

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  • Application of quinine compound in preparing anti-tubercle bacillus drugs
  • Application of quinine compound in preparing anti-tubercle bacillus drugs
  • Application of quinine compound in preparing anti-tubercle bacillus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Example 1 1403B and 1403C

[0037] The quinone compounds 1403B and 1403C of the present invention can be extracted and separated from the fermentation broth of the marine fungus Halorosellinia sp.1403, and the specific steps of the preparation method are as follows:

[0038] (1) Seed culture of the fungus Halorosellinia sp.1403 CCTCC NO: M 201018: medium by weight: glucose 0.5-1.5, yeast extract 0.05-0.15, peptone 0.1-0.3, agar 1-1.5, sodium chloride 3 -5, water 100, make a test tube slope, pick the strains into the slope, and culture at 30-35°C for 5-7 days;

[0039] (2) Fermentation culture of fungus Halorosellinia sp.1403 CCTCC NO: M 201018: Fermentation medium by weight ratio: glucose 5-15, yeast extract 1-4, peptone 0.5-4, sodium chloride 3-5, water 100, picking the cultured strains in the slant into the fermentation medium, and standing at room temperature 25-35°C for 1-2 months;

[0040] (3) filtering the above-mentioned cultivated fermented liqu...

Embodiment 2

[0046] Example 2 Determination of Absolute Concentration of 1403B and 1403C Anti-Bacillus Calmette-Guerin (BCG) by Solid Medium Dilution Method

[0047] Scrape the BCG culture from the inclined surface, add it to 3ml Middlebrook7H9 broth medium, add a small amount of glass beads, tighten the test tube cap, vibrate vigorously on the vortex shaker, and compare it with the standard McFarland turbidimetric tube (MacFarland No. 1) Turbidity, that is, to prepare a 1 mg / ml bacillus Calmette-Guerin (BCG) bacterial suspension.

[0048] Make 1403B and 1403C into high-concentration stock solution with DMSO, dilute the stock solution to the required concentration with 5% Tween-80 sterile ultrapure water, add the diluted 1403B and 1403C to 4ml Middlebrook according to the required dose 7H11 agar medium (the medium has been sterilized by high-pressure steam at 121°C for 15 minutes, cooled to 50-55°C), mixed evenly to make 1403B and 1403C, the concentrations are 60μg / mL, 40μg / mL, 30μg / mL , ...

Embodiment 3

[0051] Example 3 Determination of the Absolute Concentration of 1403B and 1403C Anti-tuberculosis Mycobacterium Standard Strain H37Rv by Solid Medium Dilution

[0052] Scrape the culture of Mycobacterium tuberculosis standard strain H37Rv from the slant, add it to 3ml Middlebrook 7H9 broth medium, add a small amount of glass beads, tighten the test tube cap, vibrate vigorously on a vortex shaker, grind with standard McFarland The turbidimetric tube (MacFarland No.1) was used for turbidity, that is, a 1 mg / ml H37Rv strain suspension was prepared.

[0053] Make 1403B and 1403C into high-concentration stock solution with DMSO, dilute the stock solution to the required concentration with 5% Tween-80 sterile ultrapure water, add the diluted 1403B and 1403C to 4ml Middlebrook according to the required dose 7H11 agar medium (the medium has been sterilized by high-pressure steam at 121°C for 15 minutes, and cooled to 50-55°C), mix well to make 60μg / mL, 40μg / mL, 30μg / mL of 1403B and 14...

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Abstract

The invention discloses an application of a quinine compound in preparing anti-tubercle bacillus drugs. A structure general formula of the quinine compound of the invention is shown in a formula (1), wherein R is H or OH. In the invention, aiming to current situations that the current tuberculosis has high morbidity, and multidrug-resistant tuberculosis (TB) strains of tubercle bacillus occur so that the morbidity and mortality of the tuberculosis are in a rising trend, and according to characteristics of anti-tubercle bacillus and tolerance tubercle bacillus activities of the quinine compound, the quinine compound is used for preparing the anti-tubercle bacillus drugs. The quinine compound of the invention is derived from marine microorganisms, is easy to be extracted, has low cost and wide application prospect, and can be used for preparing the anti-tubercle bacillus drugs.

Description

technical field [0001] The invention relates to the application field of pharmaceutical compounds, in particular to the application of quinone compounds in the preparation of anti-tuberculosis drugs. Background technique [0002] Since the discovery of cephalosporins from marine fungi in the 1960s, metabolites of marine microorganisms have increasingly become a rich source of drugs. According to Professor John's report in "Natural Product Reports" in 1010, more than 1,000 new compounds of marine natural products were discovered for the first time in 2008, and marine microorganisms were one of the main sources (accounting for about 23%). [0003] Due to the special environment of marine microorganisms, they can metabolize compounds with novel structures and have good pharmacological activities. A large number of active compounds such as anti-tumor, treatment of cardiovascular diseases, and immunomodulators have been discovered. In addition, marine microorganisms are easy to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P31/06A61K31/122
Inventor 佘志刚赖小敏林永成陈省平黄宇虹陈洪陈伊
Owner SUN YAT SEN UNIV
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